Effect of Silver Thiosulphate and Photoperiod on in Vitro Tuberization of Three Potato (Solanum Tuberosum L.) Cultivars

Abstract

The experiment was carried out to study the effect of 6 concentrations of silver thiosulphate (STS) and photoperiod on in vitro tuberization of three potato cultivars, namely, Herms, Spunta and Santana. The experiment was conducted in tissue culture laboratory, Vegetable Crops Dep., Fac. Agric., Cairo Univ. Tip meristems in each cultivar were excised and cultured on MS media. In vitro plantlets of all cultivars were multiplied as per routine by sub culturing twice. In microtuberization stage, nodal cuttings from second subculture were cultured on MS media with 0, 0.4, 0.8, 1.2, 1.6 or 2 mM of STS. The treatments were kept under dark or light conditions. STS at 0.4 and 0.8 mM recorded the tallest microplants. The highest number of leaves and branches per plantlet were obtained with 0.4mM STS. On the other hand, the STS at 2 mM recorded the lowest values of plantlet height, number of leaves and branches per plantlet. The highest percentage of microtuberization was recorded with STS at 0.4 mM under dark conditions, and at 2 mM under light one. At dark, Spunta with 1.6 mM STS produced significantly the highest number of microtubers/plantlet. Whereas at light, the maximum number of microtubers/plantlet was obtained with 0.4, 0.8 and 1.2 mM STS in Herms, Spunta and Santans, respectively. In'Spunta', application of STS at 0.4 mM under light folded the number of microtubers/plantlet 2.9 compared with dark. In Santana, number of microtubers/plantlet of 0, 0.8, 1.2, 1.6 and 2 mM STS were higher in light comparing to dark. STS applied at 0.8 and 1.2 mM produced significantly the highest value of microtuber fresh weight in Spunta and Santana, respectively under dark. In 'Spunta', the applications with 0, 1.2, 1.6 and 2 mM STS at light conditions recorded fresh weight of microtuber higher than in dark.

The final stage of micropropagation of garden strawberries

“…The success of such works has been demonstrating in the scientific literature [7]. Many problems related to the establishment of tissue culture and stabilization of the development of explants have been solving, the features of rhizogenesis have been studying, the optimal concentrations of growth regulators have been selected [8][9][10][11][12]. Much attention to the issues of genetic stability of the obtained material and the peculiarities of its development after planting in the field was paid [13][14].…”

Biochemical and Phenotypic Characterization of Salinity Potato Tolerance in Tissue Culture

“…It is understood that the negative consequences of salinity result from osmotic stress, stoppage of metabolic activity by ionic excess and imbalance, and interference of salt ions with the intake of crucial macro-and micronutrients (Gowayed et al, 2017). Plants produce antioxidants in response to osmotic stress, but they also produce compatible solutes like proline, which were formerly believed to serve as osmotic buffers, and accumulate these solutes in the cytosol (El-Sayed, 2021). A critical component of potato production is the identification and testing of commercial cultivars for salt stress tolerance using an in vitro system since different potato cultivars respond differently to salinity stress (El-Sayed et al, 2021).…”

“…Plants produce antioxidants in response to osmotic stress, but they also produce compatible solutes like proline, which were formerly believed to serve as osmotic buffers, and accumulate these solutes in the cytosol (El-Sayed, 2021). A critical component of potato production is the identification and testing of commercial cultivars for salt stress tolerance using an in vitro system since different potato cultivars respond differently to salinity stress (El-Sayed et al, 2021).…”

Silver nanoparticles potently inhibit ethylene action more than silver thiosulfate and promote microtuberization in potato (Solanum tuberosum L.) cv. Spunta

“…These results were attributed to the positive role of Ag + ions on inhibition of ethylene action in addition to increasing the accumulation of polyamines in the regenerated shoots, which recorded the highest level of a 3.6-fold increase over the control using 8 mg/l AgNO 3 without increasing the content ofH 2 O 2 , lipid peroxidation, or damage of DNA. This nding may explain the substantial success of the use of silver ions in experiments of in vitro culture for different plants; potato (Zobayed et al 2001; Ibrahim et al 2018a; El-Sayed et al 2021; Adly et al 2022), sweet potato (Gong et al 2005), canola (Khan et al 2003), sesame (Al-Shafeay et al 2011 & 2018), jojoba (Ibrahim et al 2018b), buffalograss (Fei et al 2000), faba bean (Khalafalla and Hattori 2000), sa ower (Mandal et al 2001), rice (Ibrahim and El Shihy 2012a&b), wheat (Hassan and Shahinul Islam 2021) and olive plant (Bashir et al 2022).Although, many reports con rm the positive effect of silver ions (AgNO 3 or silver thiosulfate; STS) as antiethylene on the growth of in vitro shoots(Perl et al 1988) and on the formation of microtubers in potato plants(Vreugdenhil and Van Dijk 1989;Zobayed et al 2001;El-Sayed et al 2021). Up to the present, there are only two reports on the effect of (Ag-NPs) on the growth of in vitro cultured potato plants(Ehsanpour and Nejati 2013;Baltazar Bernal et al 2023).…”