Effect of Shuangjinlian mixture on oral ulcer model in rat

Miao, Mingsan; Peng, Mengfan; Xing, Zetian; Liu, Dandan

doi:10.1016/j.sjbs.2019.02.005

Cited by 15 publications

(14 citation statements)

References 8 publications

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“…Biochemical investigation proved that the topical application of EFAM in the treatment group significantly decreased inflammation, as demonstrated by the reduction in the levels of mucosal proinflammatory TNF-α and IL-2 after days 3, 7, and 14, with a gradual decrease from one period to another. This is in agreement with previous reports [ 53 , 54 ] finding that the improvement in healing of induced oral ulcers in in vivo studies was associated with a decrease in mucosal levels of proinflammatory cytokines. Pourahmad et al [ 11 ] reported that A. maurorum as a source of flavanones could inhibit macrophage activity in oral lesions and thereby inhibit the expression of TNF-α and mediators of inflammation; this is in agreement with the present study, as EFAM improved the healing of aphthous ulcers in comparison with the vehicle-only treatment group.…”

Section: Discussionsupporting

confidence: 94%

UPLC-PDA-MS/MS Profiling and Healing Activity of Polyphenol-Rich Fraction of Alhagi maurorum against Oral Ulcer in Rats

El-Zahar

Menze

Handoussa

et al. 2022

Plants

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Camelthorn, Alhagi maurorum Boiss, family Fabaceae has long been used in African folk medicine owing to its richness in pharmacologically active metabolites. The crude extract (CEAM), ethyl acetate fraction (EFAM) and n-butanol (BFAM) fraction of A. maurorum aerial parts were investigated for their total polyphenols and oral antiulcer activity using in-vitro and in-vivo models. The major phenolic compound was isolated from the polyphenol-rich EFAM fraction and identified by conventional and spectroscopic methods of analysis as isorhamnetin-3-O-rutinoside. Furthermore, standardization of EAFM using UPLC-PDA-UV quantified isorhamnetin-3-O-rutinoside as 262.91 0.57 g/mg of the fraction. Analysis of EFAM using UPLC-PDA-MS/MS revealed tentative identification of 25 polyphenolic compounds. EFAM exhibited the most potent free radical scavenging activity against DPPH, with an IC50 (27.73 ± 1.85 μg/mL) and an FRAP value of (176.60 ± 5.21 μM Trolox equivalent (TE)/mg fraction) in comparison with CEAM and BFAM. Acetic acid-induced oral ulcers in a rat model were used to evaluate the healing properties of A. maurorum aerial parts. EFAM significantly decreased tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) by 36.4% and 50.8%, respectively, in the ulcer tissues while, CEAM and BFAM exhibited lower activity at the same dose. In addition, EFAM led to a significant (p < 0.0001) rise in the expression of proliferating cell nuclear antigen (PCNA), a cell proliferation marker. A. maurorum exhibited a potent healing effect in acetic acid-induced oral ulcers in rats by mitigating the release of pro-inflammatory cytokines and improving PCNA expression.

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Section: Discussionsupporting

confidence: 94%

UPLC-PDA-MS/MS Profiling and Healing Activity of Polyphenol-Rich Fraction of Alhagi maurorum against Oral Ulcer in Rats

El-Zahar

Menze

Handoussa

et al. 2022

Plants

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“…In the present study, the ulcerated area of subgroup (I+ve)C showed epithelial lining in the form of basal and parabasal cell layers with underlying loose lamina propria. These results are in accordance with Karavana et al [27] and Miao et al, [28] respectively. Moreover, Idrus et al, [29] reported complete healing of acetic acid induced ulceration of the buccal mucosa from 11 to 14 days and that was due to variation in healing capability and wound depth.…”

Section: Discussionsupporting

confidence: 93%

Histological and Immunohistochemical Study of the Effect of Topically Applied Phenytoin on Chemically Induced Buccal Mucosal Ulcer in Albino Rats

Wahba

Hassan

2021

Egyptian Journal of Histology

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Background: Mucosal ulcers are common lesions that arise from chemical damage to oral mucosa. Phenytoin is one of the promising wound healing agents that may have the ability to decrease the duration of the healing process of wounds. Objectives: To evaluate the effect of topical application of phenytoin on chemically induced buccal mucosal ulcer of albino rat. Material and Methods: 63 male albino rats were subjected to chemical ulcer, then rats were divided into 3 main groups. Group I (control): rats did not receive any treatment, right side buccal mucosa acted as a negative control (Group I -ve), while left side (ulcerated) served as a positive control (Group I +ve). Group II (plain gel) rats received topical application of plain gel twice daily on the ulcer from the day following ulcer induction till sacrification. Group III (phenytoin gel) rats received topical application of 1% phenytoin gel twice daily on the ulcer from the day following ulcer induction till sacrification. Each group was further divided into 3 subgroups A, B and C in which rats were sacrificed at 4, 7 and 12 days following ulcer induction respectively. Buccal mucosae were dissected and examined histologically and immunohistochemically. Results: Histologically, group I+ve showed complete epithelial degeneration at day 4, re-epithelization at day 7, complete regeneration at day 12. Group II showed complete epithelial degeneration day 4, re-epithelization started at day 7, partial regeneration at day 12. Group III showed beginning of re-epithelization at day 4, continuous epithelial lining at day 7, complete regeneration at day 12. Immunohistochemically and statistically, group III showed the highest anti-PCNA expression regarding positive epithelial cells followed by group II then group I+ve. Group I-ve showed the lowest mean. Conclusions: Topical application of phenytoin 1% accelerate healing of chemically induced ulcers through increased vascularization, decreased inflammatory cells and hastened re-epithelization.

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“…Ulcer tissue is prone to infection, inflammation, and tissue necrosis. There are many causes of oral ulcers, including immune disorders, drug stimulation, and bacterial infections [ 52 ]. Our previous research has proved the pro-regenerative effects of RL-QN15 against oral ulcers in rats [ 27 ], in the current research, we further to verify whether the load of HPDA could increase the pro-regenerative effects of RL-QN15 against oral ulcers in rats.…”

Section: Resultsmentioning

confidence: 99%

Hollow polydopamine nanoparticles loading with peptide RL-QN15: a new pro-regenerative therapeutic agent for skin wounds

Sun

Wang

et al. 2021

J Nanobiotechnol

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Background Although the treatments of skin wounds have greatly improved with the increase in therapeutic methods and agents, available interventions still cannot meet the current clinical needs. Therefore, the development of new pro-regenerative therapies remains urgent. Owing to their unique characteristics, both nanomaterials and peptides have provided novel clues for the development of pro-regenerative agents, however, more efforts were still be awaited and anticipated. Results In the current research, Hollow polydopamine (HPDA) nanoparticles were synthesized and HPDA nanoparticles loading with RL-QN15 (HPDAlR) that was an amphibian-derived peptide with obvious prohealing activities were prepared successfully. The characterization, biodistribution and clearance of both HPDA nanoparticles and HPDAlR were evaluated, the loading efficiency of HPDA against RL-QN15 and the slow-releasing rate of RL-QN15 from HPDAlR were also determined. Our results showed that both HPDA nanoparticles and HPDAlR exerted no obvious toxicity against keratinocyte, macrophage and mice, and HPDA nanoparticles showed no prohealing potency in vivo and in vitro. Interestingly, HPDAlR significantly enhanced the ability of RL-QN15 to accelerate the healing of scratch of keratinocytes and selectively modulate the release of healing-involved cytokines from macrophages. More importantly, in comparison with RL-QN15, by evaluating on animal models of full-thickness injured skin wounds in mice and oral ulcers in rats, HPDAlR showed significant increasing in the pro-regenerative potency of 50 and 10 times, respectively. Moreover, HPDAlR also enhanced the prohealing efficiency of peptide RL-QN15 against skin scald in mice and full-thickness injured wounds in swine. Conclusions HPDA obviously enhanced the pro-regenerative potency of RL-QN15 in vitro and in vivo, hence HPDAlR exhibited great potential in the development of therapeutics for skin wound healing. Graphic Abstract

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Effect of Shuangjinlian mixture on oral ulcer model in rat

Cited by 15 publications

References 8 publications

UPLC-PDA-MS/MS Profiling and Healing Activity of Polyphenol-Rich Fraction of Alhagi maurorum against Oral Ulcer in Rats

UPLC-PDA-MS/MS Profiling and Healing Activity of Polyphenol-Rich Fraction of Alhagi maurorum against Oral Ulcer in Rats

Histological and Immunohistochemical Study of the Effect of Topically Applied Phenytoin on Chemically Induced Buccal Mucosal Ulcer in Albino Rats

Hollow polydopamine nanoparticles loading with peptide RL-QN15: a new pro-regenerative therapeutic agent for skin wounds

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