Effect of Rosmarinus officinalis L. Fractions on Antimicrobial Activity against Methicillin-resistant Staphylococcus aureus (MRSA) and Resistant Genes Regulation

Abstract

This study was performed to evaluate antimicrobial activity of methanol extract from Rosmarinus officinalis L. and their fractions on methicillin-resistant Staphylococcus aureus (MRSA) and resistant gene regulation. The methanol extract of Rosmarinus officinalis L. and their hexane fractions showed the strongest antimicrobial activity against S. aureus and MRSA. To observe the morphological change of MRSA according to the hexane fraction 80 μg/mL treatment, scanning electron microscope (SEM) of MRSA were measured. The results from SEM showed decreased number of bacteria, lysis and damaged cell wall. Expressions of MecA and penicillinase were substantially decreased in a dose-dependent manner on MRSA that had been treated with methanol extract of Rosmarinus officinalis L. and their hexane fractions.

Antimicrobial Effect of Bacteriocin KU24 Produced by Lactococcus lactis KU24 against Methicillin‐Resistant Staphylococcus aureus

“…Therefore, S. aureus ATCC 33591 was used as indicator strain. Antimicrobial effect against MRSA reported on probiotic LAB (Karska‐Wysocki and others ), plant extracts of Glycyrrhiza uralensis (Lee and others ), and Rosmarinus offiicinalsis L (Choi and others ).…”

Antibacterial Activities of Fermented Sayuksan Ingredient Extracts for Multidrug-resistant Strains

“…현대과학의 발전에도 불구하고 많은 사람들이 한의학에 관 심을 표명하고 있으며, 일부 한약제제의 경우 급성이 아닌 만 성질환의 경우에 효과를 인정받고 있다. 오래전부터 지속적 으로 이용되어 온 한약재들은 기본적으로 인체 안전성을 가 지고 있고, 본질적으로 인체에 유용한 생리활성 유효성분이 있을 것으로 기대되기 때문에 한약재와 같은 천연 약물의 이 용에 관심을 두고 있다 [14][15][16][17]. 본 연구에서는 methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE)와 최근 증가하고 있는 다제내성균인 multi drug-resistant Pseudomonas aeruginosa와 multi drug-resistant Acinetobacter baumannii에 대한 항생물질 개발을 목표로 하여 감초, 지실, 시호 와 백작약 추출물의 유산균 발효를 통한 새로운 천연 항생제 로서의 개발 가능성의 기초 자료로 활용하고자 감초, 지실, 시호와 백작약 추출물의 발효 전후의 항균활성 및 항산화활 성을 비교하고자 한다.…”

xEvaluation of Anti-adherent Activity of Excretions of Irradiated Lucilia sericata Maggot and Certain Essential Oils against Some Pathogenic Bacterial Strains

“…Maggots were approved for therapeutic purpose by the US Food and Drug Administration in 2004 and maggot therapy was being used in around 1.000 medical centres in Europe and over 800 medical centres in the United State for the treatment of chronic wounds such as leg ulcers, pressure sours, diabetic necrotic ulcers, as well as infected surgical wounds, burns and trauma injuries (Nigam et al, 2006). Also, another study has shown that essential oils of medicinal plants contain bioactive compounds such as carotenoids, flavonoids, pectin, vitamins and coumarins, which have shown antibacterial, antifungal and anti-adherent activity (Choi et al, 2009).…”