Antimicrobial Effect of Bacteriocin KU24 Produced by <i>Lactococcus lactis</i> KU24 against Methicillin‐Resistant <i>Staphylococcus aureus</i>

Lee, Na‐Kyoung; Han, Eun Jin; Han, Kyoung Jun; Paik, Hyun‐Dong

doi:10.1111/1750-3841.12053

Cited by 19 publications

(15 citation statements)

References 32 publications

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“…These results suggested that AI-hemocidin 2 is capable of destroying cellular integrity and inducing significant deformation in cellular morphology. The cell surface changes induced by AI-hemocidin 2 were similar to those previously reported [ 31 , 51 ]. Peptides interact with the cellular membrane via binding to negatively charged constituents on the outer membrane, such as lipopolysaccharide (LPS), which is followed by membrane destabilization [ 52 ].…”

Section: Resultssupporting

confidence: 89%

Antibacterial Activity of AI-Hemocidin 2, a Novel N-Terminal Peptide of Hemoglobin Purified from Arca inflata

Zhu

Wang³

et al. 2017

Marine Drugs

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The continued emergence of antibiotic resistant bacteria in recent years is of great concern. The search for new classes of antibacterial agents has expanded to non-traditional sources such as shellfish. An antibacterial subunit of hemoglobin (Hb-I) was purified from the mantle of Arca inflata by phosphate extraction and ion exchange chromatography. A novel antibacterial peptide, AI-hemocidin 2, derived from Hb-I, was discovered using bioinformatics analysis. It displayed antibacterial activity across a broad spectrum of microorganisms, including several Gram-positive and Gram-negative bacteria, with minimal inhibitory concentration (MIC) values ranging from 37.5 to 300 μg/mL, and it exhibited minimal hemolytic or cytotoxic activities. The antibacterial activity of AI-hemocidin 2 was thermostable (25–100 °C) and pH resistant (pH 3–10). The cellular integrity was determined by flow cytometry. AI-hemocidin 2 was capable of permeating the cellular membrane. Changes in the cell morphology were observed with a scanning electron microscope. Circular dichroism spectra suggested that AI-hemocidin 2 formed an α-helix structure in the membrane mimetic environment. The results indicated that the anti-bacterial mechanism for AI-hemocidin 2 occurred through disrupting the cell membrane. AI-hemocidin 2 might be a potential candidate for tackling antibiotic resistant bacteria.

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Section: Resultssupporting

confidence: 89%

Antibacterial Activity of AI-Hemocidin 2, a Novel N-Terminal Peptide of Hemoglobin Purified from Arca inflata

Zhu

Wang³

et al. 2017

Marine Drugs

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“…Treatment with LAB bacteriocins is an effective and safe way to inhibit S. aureus growth in food. Many researchers have shown that LAB bacteriocins have anti-MRSA ability [ 11 , 24 , 25 ]. However, only a few studies have been performed to investigate the LAB bacteriocins against other drug-resistant S. aureus such as anticiprofloxacin, anticefoxitin, and antigentamicin [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Purification, Characterization, and Mode of Action of Pentocin JL-1, a Novel Bacteriocin Isolated from Lactobacillus pentosus, against Drug-Resistant Staphylococcus aureus

Jiang

Zou

Cheng

et al. 2017

BioMed Research International

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Staphylococcus aureus and its drug-resistant strains, which threaten public health and food safety, are in need of effective control by biopreservatives. A novel bacteriocin, pentocin JL-1, produced by Lactobacillus pentosus that was isolated from the intestinal tract of Chiloscyllium punctatum, was purified by a four-step chromatographic process. Mass spectrometry based on MALDI-TOF indicated that pentocin JL-1 has a molecular mass of 2987.23 Da. Only six of the twenty-five amino acids could be identified by Edman degradation. This bacteriocin is thermostable and tolerates a pH range of 5–7. Also, it is sensitive to proteinase K, trypsin, pepsin, and alkaline protease. This bacteriocin has a broad inhibitory spectrum against both Gram-positive and Gram-negative strains and in particular is effective against multidrug-resistant S. aureus. Additionally, we showed that the cell membrane is the target of pentocin JL-1 against methicillin-resistant S. aureus (MRSA), causing a loss of proton motive force. Furthermore, pentocin JL-1 has a drastic impact on the structure and integrity of MRSA cells. These results suggest that pentocin JL-1 has potential as a biopreservative in the food industry.

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“…Lactic acid bacteria produce antimicrobial compounds like organic acids, diacetyl, hydrogen peroxide, and proteinaceous bacteriocins ( Lee et al ., 2013 ). Bacteriocins are antimicrobial peptides or proteins produced by mainly lactic acid bacteria; these compounds are small and ribosomally synthesized.…”

Section: Natural Preservatives From Microorganismsmentioning

confidence: 99%

Status, Antimicrobial Mechanism, and Regulation of Natural Preservatives in Livestock Food Systems

Lee¹,

Paik²

2016

Korean Journal for Food Science of Animal Resources

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This review discusses the status, antimicrobial mechanisms, application, and regulation of natural preservatives in livestock food systems. Conventional preservatives are synthetic chemical substances including nitrates/nitrites, sulfites, sodium benzoate, propyl gallate, and potassium sorbate. The use of artificial preservatives is being reconsidered because of concerns relating to headache, allergies, and cancer. As the demand for biopreservation in food systems has increased, new natural antimicrobial compounds of various origins are being developed, including plant-derived products (polyphenolics, essential oils, plant antimicrobial peptides (pAMPs)), animal-derived products (lysozymes, lactoperoxidase, lactoferrin, ovotransferrin, antimicrobial peptide (AMP), chitosan and others), and microbial metabolites (nisin, natamycin, pullulan, ε-polylysine, organic acid, and others). These natural preservatives act by inhibiting microbial cell walls/membranes, DNA/RNA replication and transcription, protein synthesis, and metabolism. Natural preservatives have been recognized for their safety; however, these substances can influence color, smell, and toxicity in large amounts while being effective as a food preservative. Therefore, to evaluate the safety and toxicity of natural preservatives, various trials including combinations of other substances or different food preservation systems, and capsulation have been performed. Natamycin and nisin are currently the only natural preservatives being regulated, and other natural preservatives will have to be legally regulated before their widespread use.

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Antimicrobial Effect of Bacteriocin KU24 Produced by Lactococcus lactis KU24 against Methicillin‐Resistant Staphylococcus aureus

Cited by 19 publications

References 32 publications

Antibacterial Activity of AI-Hemocidin 2, a Novel N-Terminal Peptide of Hemoglobin Purified from Arca inflata

Antibacterial Activity of AI-Hemocidin 2, a Novel N-Terminal Peptide of Hemoglobin Purified from Arca inflata

Purification, Characterization, and Mode of Action of Pentocin JL-1, a Novel Bacteriocin Isolated from Lactobacillus pentosus, against Drug-Resistant Staphylococcus aureus

Status, Antimicrobial Mechanism, and Regulation of Natural Preservatives in Livestock Food Systems

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