Effect of Resveratrol, a Dietary-Derived Polyphenol, on the Oxidative Stress and Polyol Pathway in the Lens of Rats with Streptozotocin-Induced Diabetes

Abstract

Resveratrol is found in grapes, apples, blueberries, mulberries, peanuts, pistachios, plums and red wine. Resveratrol has been shown to possess antioxidative activity and a variety of preventive effects in models of many diseases. The aim of the study was to investigate if this substance may counteract the oxidative stress and polyol pathway in the lens of diabetic rats. The study was conducted on the rats with streptozotocin-induced type 1 diabetes. After the administration of resveratrol (10 and 20 mg/kg po for 4 weeks), the oxidative stress markers in the lens were evaluated: activity of superoxide dismutase, catalase and glutathione peroxidase, as well as levels of total and soluble protein, level of glutathione, vitamin C, calcium, sulfhydryl group, advanced oxidation protein products, malonyldialdehyde, Total Oxidant Status and Total Antioxidant Reactivity. The obtained results indicate that the administration of resveratrol to the diabetic rats shows antioxidative properties. It is not a result of antiglycaemic activity but resveratrol probably directly affects the antioxidative system. Resveratrol did not affect the polyol pathway in the lens of diabetic rats. Our results may indirectly indicate benefits of consumption of foods as well as dietary supplements containing resveratrol in diminishing oxidative stress in lenses of individuals suffering from diabetes mellitus.

Chrysin Reduces Oxidative Stress but Does Not Affect Polyol Pathway in the Lenses of Type 1 Diabetic Rats

“…The C and DM rats received water by gavage in a volume corresponding to their current body mass (1 mL of water per 1 kg of body mass). This research shares the control C and DM groups with our previously presented studies [29][30][31][32].…”

Efficacy and Safety of Resveratrol in Type 1 Diabetes Patients: A Two-Month Preliminary Exploratory Trial

“…Our results are consistent with preclinical studies, which have reported that resveratrol reduced the level of oxidative stress markers and improved antioxidant defenses. A reduction in oxidative stress markers, such as superoxide anion, hydroxyl radical, hydrogen peroxide, MDA, 8-isoprostane, 8-hydroxydeoxyguanine, nitro-tyrosine, and reduced/oxidized glutathione [30][31][32][33][34], as well as an improvement in the activities of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase, has been observed in the setting of diabetes [33,35,36]. Resveratrol also attenuates autoimmune-mediated destruction of insulin-releasing pancreatic β-cells.…”

Health benefits of resveratrol administration

“…Resveratrol intake by diabetic rats at a dose of 5 mg/ kg/day leads to normalization of antioxidant status, exacerbated by oxidative stress induced by hyperglycemia (Hussein & Mahfouz, 2016). Administration of resveratrol at a dose of 10 or 20 mg/kg for 4 weeks in rats with a streptozotocin-induced diabetes results in a reduction in the content of AOPP and MDA as well as catalase (CAT) and superoxide dismutase (SOD) activity in the lens compared to untreated diabetic rats (Sedlak et al, 2018). Resveratrol-treated animals have reduced production of ROS, elevated membrane potential, and inhibition of cytochrome c release from the inner mitochondrial membrane (Zhang et al, 2018).…”