Effect of red mud addition on tetracycline and copper resistance genes and microbial community during the full scale swine manure composting

Abstract

h i g h l i g h t sARGs could be effectively removed through the thermophilic composting process. The main contributor of reducing ARGs abundance was pile temperature. Bacterial community was the main driver to reduction of ARGs abundance. Swine manure has been considered as the reservoir of antibiotic resistance genes (ARGs). Composting is one of the most suitable technologies for treating livestock manures, and red mud was proved to have a positive effect on nitrogen conservation during composting. This study investigated the abundance of eight tetracycline and three copper resistance genes, the bacterial community during the full scale swine manure composting with or without addition of red mud. The results showed that ARGs in swine manure could be effectively removed through composting (reduced by 2.4 log copies/g TS), especially during the thermophilic phase (reduced by 1.5 log copies/g TS), which the main contributor might be temperature. Additionally, evolution of bacterial community could also have a great influence on ARGs. Although addition of red mud could enhance nitrogen conservation, it obviously hindered removal of ARGs (reduced by 1.7 log copies/g TS) and affected shaping of bacterial community during composting.

Effects of Copper Addition on Copper Resistance, Antibiotic Resistance Genes, and intl1 during Swine Manure Composting

“…Pseudomonas species are highly resistant to and tolerant of copper (Liu et al, 2015), which may be attributed to the presence of the operon cop regulating the copper concentration in the cell as well as their ability to produce a large quantity of exopolymer to reduce the toxicity of copper in the soil (Kunito et al, 2001; Andreazza et al, 2012). The genus Corynebacterium includes potential human pathogens (Mohammadi et al, 2013), and Wang et al (2016) claimed that Corynebacterium has a positive correlation with pco genes. In addition, Bacteroides were enriched in Cu200 and Cu2000 after 14 days ( Figure 3 ).…”

The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health

“…Fortunately, with recent technological advances such as aerobic composting and anaerobic digest, residues of antibiotics and ARGs are profoundly reduced from manure before being applied to the soil (Wei, Qian, Gu, Wang, & Duan, 2016). Aerobic composting is one of the widely recognized methods used during manure production because of its simplicity, efficiency, and economic-feasibility (Arikan, Mulbry, & Rice, 2009;Wang et al, 2016). However, the elimination efficacies of antibiotics and ARGs from the manure vary with different treatment procedures, manure types, and sites in a few scenarios (Zhao, Dong, & Wang, 2010;Zhu et al, 2013).…”

Human and veterinary antibiotics during composting of sludge or manure: Global perspectives on persistence, degradation, and resistance genes

“…thermophilic stage (1.5 log copies/g dry matter). In these papers, a positive correlation was found between the antibiotic resistance genes reduction during composting and the temperature [102,[104][105][106][107][108]. Additionally, temperature was identified as an important parameter related to the transfer of a mobile plasmid (pIE732) [109].…”