Effect of quercetin, rutin, naringenin and epicatechin on lipid peroxidation induced in human sperm

Arruda

Arq. Bras. Med. Vet. Zootec.

et al. 2016

The aim of this study was to evaluate the effect of different concentrations of trans-resveratrol or quercetin on the ability of goat sperm to withstand being frozen. Six pools of semen obtained from six male goats were processed with different concentrations of resveratrol or quercetin (Experiment 1: 0, 15, 25, 50, 75 or 100µM resveratrol; Experiment 2: 0, 15, 25, 50, 75 or 100µM quercetin) and frozen. After thawing, the semen was evaluated for sperm kinematics, plasma membrane and acrosome integrity, morphology and oxidative stress following 0 and 1h of incubation. Immediately after thawing (0h), wobble (oscillation index) in the groups treated with 100µM of quercetin or resveratrol was lower (P<0.05) than in those treated with 0 and 25µM resveratrol and 0µM quercetin, respectively. After 1h of incubation, the total motility in treatments with 15, 50 and 75µM quercetin, as well as the plasma membrane integrity in all quercetin concentrations were lower (P<0.05) than at 0h. In opposition, the linearity of semen samples treated with 100µM quercetin and the straightness of those treated with 75 and 100µM quercetin were lower (P<0.05) at 0h than at 1h after thawing. Thus, it can be concluded that resveratrol and quercetin at high concentrations (100µM) transiently reduce the wobble of goat sperm submitted to frozen storage, and that quercetin (75 and 100µM) increases the linearity and straightness over time, which can be favorable for fertility.

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

High resveratrol or quercetin concentrations reduce the oscillation index of frozen goat semen

Arruda

Arq. Bras. Med. Vet. Zootec.

et al. 2016

“…In Japan, the average quercetin intakes were 16.2 mg/day [3]. The antibacterial [4], antiviral [5], antiallergic [6], anticarcinogenic [7,8] and antioxidant [9,10] properties of quercetin were widely studied and described. Moreover, quercetin can effectively chelate metal ions.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic, thermogravimetric and biological studies of Na(I), Ni(II) and Zn(II) complexes of quercetin

Kalinowska

Świderski

Matejczyk

et al. 2016

J Therm Anal Calorim

The Na(I), Zn(II) and Ni(II) complexes with quercetin have been synthesized. The composition of these compounds was established by means of elemental and thermogravimetric analyses. To study the molecular structure of synthesized compounds, many miscellaneous analytical methods, which complement one another, were used: infrared (FT-IR), Raman (FT-Raman) and electronic absorption UV/VIS spectroscopy. For studying the cytotoxic and genotoxic activity of synthesized compounds, bioreporter strain of Escherichia coli K-12 recA::gfpmut2 has been used.

“…Moretti et al studied the antioxidant effects of flavonoids and showed their positive results on spermatogenesis. The research on flavonoid's subcategory, such as luteolin, catechin, fiestin, Moretti et al emphasized that their role in improving spermatogenesis mostly was dependent on their antioxidant effect (34). Likewise, Zingiber, Cinnamon, Citrullus lanatus and Daucus carota' seeds had significant effects on oligospermia, regulation of blood testosterone levels, increased levels of SOD and decreased levels of MDA.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Compound Herbal Remedy Used in Male Infertility on Spermatogenesis and Pregnancy Rate

Ouladsahebmadarek¹,

Giasi²,

Khaki³

et al. 2015

IJWHR

IntroductionInfertility is defined by the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse which involves 10%-15% of couples. Male factor is responsible for 40% of infertility cases. The leading cause of male infertility is impaired sperm production caused by various factors such as infections, genetic disorders, medications, aging, social and environmental factors (1-5). Oxidative damage due to free radicals is one of the major causes of idiopathic oligospermia, even with undesirable impact on the structure of sperm produces abnormal sperm. For example, in diabetic patients by producing reactive oxygen species (ROS), free radicals of nitric oxide cause oxidative stress and thus result in testicular insufficiency through affecting spermatogenesis and estrogenic pathways (6-8). Varicocele which has been observed in 19% to 41% of infertile men is a common cause of male infertility (9-11). Increased levels of ROS and oxidative levels (oxidative stress) is one of the proposed mechanisms for impaired spermatogenesis. Vacuole formation in testis tissue is a sign of oxidative stress and is associated with aging which can be neutralized by antioxidants (12)(13)(14)(15). Lack of vitamin A, elements such as flavonoids, carnitine, folate, zinc and selenium and overall reduction of antioxidants in the diet can be one of the reasons for infertility especially oligospermia and asthenozoospermia in humans (16-18). Antioxidants like vitamin E, vitamin C, glutathione, coenzyme Q10 and natural polyphenols such as flavonoids and phenolic acids by reducing the damage caused by free radicals, strengthening blood-testis barrier and protecting and repairing sperm DNA, are effective in the treatment of male infertility (19-21). Assisted reproductive techniques (ART) such as in vitro fertilization (IVF), zygote intra-fallopian transfer (ZIFT) and intra-cytoplasmic sperm insemination (ICSI) has solved many problems of infertility; however, due to high cost, they are not affordable by many of infertile couples. On the other hand, there are effective medications which are less expensive and available for a wide range of couples. The bioactive compounds in plant extracts are highly regarded because it is derived from natural sources and compatible with vital systems. Using herbal medicine to increase fertility and improve factors such as hormonal imbalance, oligospermia, low motility of sperm, prostatitis, varicocele, etc has been considered for a long time. Previous studies on laboratory animals indicated that plants such as Allium cepa, Zingiber officinale, Ocimum basilicum, Cinnamomum verum, Citrus sinensis' peel, Citrullus lanatus and Daucus carota' seeds as a rich source of Abstract Objectives: TNowadays, male infertility is one of the most common issues in the medical field and the main reason for it is impaired sperm production. Assisted Reproductive Techniques (ARTs) used for treatment of infertile couples are too costly. Currently, using herbal medicine due to lower side effe...