Effect of pure and oxidized cholesterol-rich diets on some biochemical parameters in rats

Kanhal, M. A. Al; Ahmad, Faiyaz; Ainoon, O; Arif, Zarina; Orf, S. Al; Murshed, K.S. Al

doi:10.1080/0963748021000044723

Cited by 19 publications

(16 citation statements)

References 33 publications

(37 reference statements)

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“…A diet high in cholesterol causes an increase in oxidative stress in the liver and results in increases in AST and ALT levels. 23) Rats fed with the diosgenin-supplemented diet at the level of 0.1% and 0.5% respectively reduced plasma AST by about 62% and 75%, and plasma ALT by 64% and 78% compared to the animals fed on the highcholesterol control diet. This suggests that the addition of diosgenin at the level of 0.1% or 0.5% may have a liver protective effect under high-cholesterol diet conditions.…”

Section: Resultsmentioning

confidence: 95%

Antioxidative and Hypolipidemic Effects of Diosgenin, a Steroidal Saponin of Yam (Dioscorea spp.), on High-Cholesterol Fed Rats

Son

Kim

Sohn

et al. 2007

Bioscience, Biotechnology, and Biochemistry

227

125

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Diosgenin (a steroidal saponin of yam) has long been used as a raw material for the industrial production of steroid drugs, and reported to have a hypocholesterolemic effect by suppressing cholesterol absorption and increasing cholesterol secretion. Oxidative stress has been suggested as a main risk factor in the development of atherosclerosis. The aim of this study is to investigate the possible hypolipidemic and antioxidative effect of diosgenin on rats fed with a high-cholesterol diet supplemented with either 0.1% or 0.5% diosgenin for 6 weeks. We measured the lipid profile in the plasma and liver, lipid peroxidation and antioxidative enzyme activities in the plasma, erythrocyte and gene expression of antioxidative enzymes in the liver, and the oxidative DNA damage in lymphocytes. Diosgenin showed a decrease in the plasma and hepatic total cholesterol levels, but increased the plasma high-density lipoprotein (HDL) cholesterol level. Erythrocyte TBARS and lymphocyte DNA damage measured by the comet assay were decreased in the diosgenin supplemented group. Furthermore, diosgenin feeding enhanced the resistance to lymphocyte DNA damage caused by an oxidant challenge with H(2)O(2). The antioxidative enzyme activities were also affected by diosgenin supplementation. Total superoxide dismutase (SOD) in the plasma and liver, glutathione peroxidase (GSH-Px) in erythrocytes, and catalase (CAT) in erythrocytes and liver were significantly increased in the 0.5% diosgenin group. The expression of antioxidative enzymes was up-regulated by diosgenin, the expression of GSH-Px being the highest in the 0.5% diosgenin group. These results suggest that diosgenin could be a very useful compound to control hypercholesterolemia by both improving the lipid profile and modulating oxidative stress.

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Section: Resultsmentioning

confidence: 95%

Antioxidative and Hypolipidemic Effects of Diosgenin, a Steroidal Saponin of Yam (Dioscorea spp.), on High-Cholesterol Fed Rats

Son

Kim

Sohn

et al. 2007

Bioscience, Biotechnology, and Biochemistry

227

125

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“…After an adaptation period, the animals were randomly divided into three groups of six rats each. The design of the experimental diets in this work is similar to that described by Al-Kanhal et al [15]; for two groups, the standard diet was supplemented with either 1% (w/w) pure cholesterol (Chol group) or 1% (w/w) of the oxidized cholesterol mixture (COPs group) described above. These diets were fed to the rats for 8 weeks.…”

Section: Animals and Dietsmentioning

confidence: 99%

Alteration of Some Inflammatory Biomarkers by Dietary Oxysterols in Rats

et al. 2012

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Oxysterols are structurally similar to cholesterol, but are characterized by one or more additional oxygen-containing functional groups. These compounds are implicated in inflammation given their ability to cause irreversible damage to vascular cells. The aim of this study was to study the alteration of some inflammatory biomarkers in Wistar rats in response to dietary oxysterols. Eighteen rats were randomly divided into three groups of six rats each. A standard diet supplemented with 1% (w/w) pure cholesterol (Chol group) or 1% (w/w) of an oxidized cholesterol mixture (COPs group) was fed for 8 weeks. Blood serum was separated; abdominal, pericardial, and epididymal adipose tissue was removed carefully. The COPs subjects exhibited significant increase in blood pressure and serum triacylgycerols as well as increased body fat index and pericardic, abdominal, and epididymal adipose tissue. These effects were accompanied by elevated circulating levels of plasma high-sensitivity C-reactive protein, tumor necrosis factor alpha, and resistin. We suggest that dietary oxysterols have an important pro-inflammatory effect.

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“…The GPT level was significantly lower in HEMC mice compared with NC and HF mice. The GOT and GPT enzymes are important indicators of tissue damage and their high activities have been reported in response to oxidative stress (Kanhal et al, 2002;Ozaki, Fuchinoue, Teraoda, & Ota, 1995). Hence, reduced activities of GOT and GPT indicate decreased hepatic oxidative stress.…”

Section: Plasma Lipid Profilementioning

confidence: 99%

Antihyperlipidemic effects of hydroxyethyl methylcellulose with varying viscosity in mice fed with high fat diet

Ban¹,

Rico²,

Um³

et al. 2012

Food Research International

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Effect of pure and oxidized cholesterol-rich diets on some biochemical parameters in rats

Cited by 19 publications

References 33 publications

Antioxidative and Hypolipidemic Effects of Diosgenin, a Steroidal Saponin of Yam (Dioscorea spp.), on High-Cholesterol Fed Rats

Antioxidative and Hypolipidemic Effects of Diosgenin, a Steroidal Saponin of Yam (Dioscorea spp.), on High-Cholesterol Fed Rats

Alteration of Some Inflammatory Biomarkers by Dietary Oxysterols in Rats

Antihyperlipidemic effects of hydroxyethyl methylcellulose with varying viscosity in mice fed with high fat diet

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