Effect of propolis in gastric disorders: inhibition studies on the growth ofHelicobacter pyloriand production of its urease

Abstract

There is considerable interest in alternative approaches to inhibit Helicobacter pylori (H. pylori) and thus treat many stomach diseases. Propolis is a pharmaceutical mixture containing many natural bioactive substances. The aim of this study was to use propolis samples to treat H. pylori. The anti-H. pylori and anti-urease activities of 15 different ethanolic propolis extracts (EPEs) were tested. The total phenolic contents and total flavonoid contents of the EPE were also measured. The agar-well diffusion assay was carried out on H. pylori strain J99 and the inhibition zones were measured and compared with standards. All propolis extracts showed high inhibition of H. pylori J99, with inhibition diameters ranging from 31.0 to 47.0 mm. Helicobacter pylori urease inhibitory activity was measured using the phenol-hypochlorite assay; all EPEs showed significant inhibition against the enzyme, with inhibition concentrations (IC 50 ; mg/mL) ranging from 0.260 to 1.525 mg/mL. The degree of inhibition was related to the phenolic content of the EPE. In conclusion, propolis extract was found to be a good inhibitor that can be used in H. pylori treatment to improve human health.

A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys

“…Helicobacter pylori, an anaerobic bacterium, caused numerous inflammations that can cause gastritis, gastric ulcer, peptic ulcer, reflux and eventually cancer by settling in the gastric mucosa, stomach and duodenum. For survival, the bacteria in the gastric systems need urease enzyme 34 .…”

Propolis: The future therapy against Helicobacter pylori-mediated gastrointestinal diseases

“…It blocks the binding of substrate to the active site of PDF while exerting no effect on metal ion binding or on different metal-dependent catalytic processes of host tissues (Cui et al, 2013). Baltas et al (2016) showed in an in vitro study that ethanolic extracts of propolis containing different phenolic compounds (Baik et al, 1996). The survival of such damaged cells is an important factor that determines a person's health (Smoot et al, 2000).…”

Antimicrobial and antiviral properties of different types of propolis

“…The growth inhibition zones of these bacteria caused by the ethanol extracts of propolis were greater than those of Amoxicillin antibiotic and 70% ethanol (31-47, 40, and 10 mm, respectively). In addition, EEP also inhibits the activity of urease produced by H. pylori, and thus can provide effective support for the treatment of gastro-duodenal diseases [Baltas et al 2016].…”