Effect of plant growth regulators on direct regeneration and callus induction from Sargassum polycystum C. Agardh

Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production.

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“…explants cultured on MSO and WPMO media. In addition, the MSO medium failed to induce callus from Sargassum polycystum explants [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

Zaman

Azzeme

Ramle

et al. 2020

Plants

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“…The 1990s brought together concerted efforts to develop background technologies for the micropropagation of a wide range of seaweeds, and several protocols for routine callus induction and regeneration are now available in the literature [ 57 , 184 , 185 , 186 , 187 ]. Clonal propagation of somatic embryos of seaweed was conducted in the hope of commercially cultivating them with the desired traits.…”

Section: Existing and Future Mitigation Strategiesmentioning

confidence: 99%

A Retrospective Review of Global Commercial Seaweed Production—Current Challenges, Biosecurity and Mitigation Measures and Prospects

Sugumaran

Padam²,

Yong

et al. 2022

IJERPH

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Commercial seaweed cultivation has undergone drastic changes to keep up with the increasing demand in terms of the quantity and quality of the algal biomass needed to meet the requirements of constant innovation in industrial applications. Diseases caused by both biotic and abiotic factors have been identified as contributing to the economic loss of precious biomass. Biosecurity risk will eventually affect seaweed production as a whole and could cripple the seaweed industry. The current review sheds light on the biosecurity measures that address issues in the seaweed industry pushing towards increasing the quantity and quality of algal biomass, research on algal diseases, and tackling existing challenges as well as discussions on future directions of seaweed research. The review is presented to provide a clear understanding of the latest biosecurity developments from several segments in the seaweed research, especially from upstream cultivation encompassing the farming stages from seeding, harvesting, drying, and packing, which may lead to better management of this precious natural resource, conserving ecological balance while thriving on the economic momentum that seaweed can potentially provide in the future. Recommended breeding strategies and seedling stock selection are discussed that aim to address the importance of sustainable seaweed farming and facilitate informed decision-making. Sustainable seaweed cultivation also holds the key to reducing our carbon footprint, thereby fighting the existential crisis of climate change plaguing our generation.

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“…This morphological response is a notable example of cell totipotency, and it can be manipulated for sustainable seedling production (Wang et al 2016). Direct regeneration has successfully been employed to produce consistent seedlings from species such as Grateloupia filicina , K. alvarezii (Yong et al 2014b), and Sargassum polycystum (Muhamad et al 2018) in Provassoli's Enriched Seawater (PES) or f/2 liquid media for various applications. Although spontaneous regeneration allows for increased seed stock production, supplementing culture media with plant growth regulators (PGRs) and biostimulants enhances direct shoot formation from seaweed explants even more, resulting in a high number of uniform seedlings in a shorter time (Yunque et al 2011, Hurtado andCritchley 2018).…”

Section: Direct Regenerationmentioning

confidence: 99%

Recent advances in seaweed seedling production: a review of eucheumatoids and other valuable seaweeds

Jiksing¹,

Ongkudon²,

Thien³

et al. 2022

Algae

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Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.

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Effect of plant growth regulators on direct regeneration and callus induction from Sargassum polycystum C. Agardh

Cited by 17 publications

References 41 publications

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

A Retrospective Review of Global Commercial Seaweed Production—Current Challenges, Biosecurity and Mitigation Measures and Prospects

Recent advances in seaweed seedling production: a review of eucheumatoids and other valuable seaweeds

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