Effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb

Boltenkov, Eugeny V.; Миронова, Л. Н.; Зарембо, Е. В.

doi:10.1134/s1062359007050044

Cited by 9 publications

(11 citation statements)

References 29 publications

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“…Callus regeneration induced by a cytokinin alone or in combination with an auxin is reported in several systems including some legumes. This includes Arachis hypogaea (Venkatachalam et al 1999), Hypericum perforatum (Pretto and Santar'em 2000), Vitex negundo (Rani and Nair 2006), Iris ensata (Boltenkov et al 2007), Vigna subterranea (Kone et al 2006), Albizia odoratissima (Rajeswari and Paliwal 2008), and Phaseolus lunatus (Kanchiswamy and Maffei 2008). BA-and IAA-induced callus regeneration was reported in Acacia sinuata (Vengadesan et al 2000), Citrullus lanatus (Chaturvedi and Bhatnagar 2001) and Argyrolobium roseum (Khanna et al 2006).…”

Section: Resultsmentioning

confidence: 92%

Pulvinus: an ideal explant for plant regeneration in Caesalpinia bonduc (L.) Roxb., an important ethnomedicinal woody climber

2011

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This study demonstrates the morphogenic potential of pulvinus, an important organ situated at the base of the petiole or rachis of leguminous plants. Plant regeneration via pulvinus-derived calli of Caesalpinia bonduc has been achieved. Organogenic calli have been derived from the explant 45 days after culture on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with 6-benzylaminopurine (BA). Optimum callus induction (100%) occurred when the pulvini were cultured on MS medium fortified with 6 mg l -1 2,4-D and 1 mg l -1 BA. The highest shoot induction was obtained when the calli were transferred to MS medium supplemented with 5 mg l -1 BA and 1 mg l -1 indole-3-acetic acid (IAA). On this medium, 87% cultures responded with an average number of 4.2 shoots per culture. The maximum root induction from the regenerated shoots was observed on half strength MS medium containing 6 mg l -1 indole-3-butyric acid (IBA). Here 100% shoots rooted with a mean number of 6.3 roots per shoot. The regenerated plantlets were acclimatized and subsequently showed normal growth. This efficient protocol will be helpful for propagating elite clones on a mass scale and could be utilized for genetic transformation study.

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Section: Resultsmentioning

confidence: 92%

Pulvinus: an ideal explant for plant regeneration in Caesalpinia bonduc (L.) Roxb., an important ethnomedicinal woody climber

2011

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“…In treatments including BAP, shoot regeneration response changed with species and I. schachtii was found to be better than I. sari. The promotive effect of BAP alone or in combination with NAA-inducing callus and adventitious bulblet regeneration was previously reported in other Iris species (Kawase et al, 1995;Suzhen et al, 1999;Shibli and Ajlouni, 2000;Boltenkov and Zarembo, 2005;Boltenkov et al, 2007).…”

Section: Discussionmentioning

confidence: 74%

“…However, with shoots cultured in medium without PGRs, rooting success was only 52.5% in both species. Boltenkov et al (2007) suggested that PGRs were required for root initiation in Iris ensata. Acclimatization is a critical stage and often associated with slow growth and significant plant loss.…”

Section: Discussionmentioning

confidence: 99%

“…A recent trend of using wild plants for ornamental purposes by collecting them from their natural habitats could lead to the extinction of wild plants (Prado et al, 2010). Iris species can be propagated from seeds, bulbs, or rhizomes with a low propagation speed like other geophytes (Jevremovic and Radojevic, 2002;Boltenkov et al, 2007). Therefore, in recent years, a number of studies have reported in vitro propagation of Iris species such as I. ensata (Yabuya et al, 1991;Boltenkov et al, 2007), I. germanica (Shimizu et al, 1997;Wang et al, 1999aWang et al, , 1999b, I. nigricans (Shibli and Ajlouni, 2000), I. pumila (Jevremovic and Radojevic, 2002), I. ensata, I. setosa, I. sanguinea (Boltenkov and Zarebno, 2005), I. hollandica (Fidalgo et al, 2005), I. atrofusca, I. petrana, I. vartanii (AlGabbiesh et al, 2006, and I. adriatica (Keresa et al, 2009) from a range of explants.…”

Section: Introductionmentioning

confidence: 99%

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Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Uzun¹,

Ilbaş²,

İpek³

et al. 2014

Turk J Agric For

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“…1F). 다른 붓꽃과 식물체에서도 오옥신과 사이토카이닌의 혼합배지에서 재분화된 신초 로부터 뿌리의 유도는 생장조절물질을 첨가하지 않은 배 지에서 뿌리의 발달이 가장 효과적이었다고 보고하였다 (Wang et al 1999;Zheng et al 1999;Boltenkov et al 2007). 재분화된 식물체는 순화처리를 위해서 생장조절물질 과 당을 첨가하지 않은 MS 기본배지에 옮겨 2주간 배양 하였다.…”

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Effect of plant growth regulators on plant regeneration from the Belamcanda chinensis

Kwon¹,

Yoo²,

Yoon

2010

Journal of Plant Biotechnology

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To establish the optimum conditions of in vitro plant regeneration, the leaf, rhizome, and root explants of Belamcanda chinensis were cultured on the MS medium supplemented with different concentration of 2,4-D and BA. The callus induction was more effective in the root explants than the leaf and rhizome explants, and was the best on MS medium containing 3.0 mg/L 2,4-D or 1.0 mg/L 2,4-D and 3.0 mg/L BA. The highest numbers of shoots were regenerated when callus were cultured on MS medium containing 3.0 mg/L 2,4-D for 4 weeks. However, the multiple shoots were induced on MS medium supplemented with the combination of 2,4-D and BA. The normal root formation from shoot was effective on the MS medium lacking any plant growth regulators. For acclimatization, the regenerated plantlets were cultured on MS medium without sucrose and plant growth regulators for 2 weeks, and then transferred to the pot.

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Effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb

Cited by 9 publications

References 29 publications

Pulvinus: an ideal explant for plant regeneration in Caesalpinia bonduc (L.) Roxb., an important ethnomedicinal woody climber

Pulvinus: an ideal explant for plant regeneration in Caesalpinia bonduc (L.) Roxb., an important ethnomedicinal woody climber

Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Effect of plant growth regulators on plant regeneration from the Belamcanda chinensis

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