Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Uzun, Sati; Ilbaş, Ali; İpek, Ahmet; Arslan, Neşet; Barpete, Surendra

doi:10.3906/tar-1306-47

Cited by 11 publications

(13 citation statements)

References 30 publications

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“…Acclimatization is a critical stage that is often associated with slow growth and signifi cant plant loss (Uzun et al, 2014). In this study, many roots (3.9) formed on MS medium with 0.1 mg/l BA and 0.02 mg/l NAA (ARM-6), while 2.40 roots/shoot formed on MS medium supplemented with 2 mg/l Kin and 0.5 mg/l NAA (Esizad et al, 2012), 2.55 roots on MS medium fortifi ed with 1 mg/l Kin and 0.5 mg/l NAA (Kaviani, 2014), and 62.0 roots on B5 medium with 1.5 mg/l NAA (Mousavi et al, 2012a).…”

Section: Discussionmentioning

confidence: 99%

Leaf-derived organogenesis in vitro for mass propagation of lisianthus (Eustoma grandiflorum (Raf.) Shinn

Winarto¹,

Rachmawati²,

Silva³

2015

Emir. J. Food Agric

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Section: Discussionmentioning

confidence: 99%

Leaf-derived organogenesis in vitro for mass propagation of lisianthus (Eustoma grandiflorum (Raf.) Shinn

Winarto¹,

Rachmawati²,

Silva³

2015

Emir. J. Food Agric

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“…Mass propagation of D.philippinensis through tissue culture is an efficient technique to conserve an endemic plant species using MS medium [5]. The growth of plant tissue is achieved with the desired culture media composition [8,9,10].…”

Section: Resultsmentioning

confidence: 99%

“…Thus, propagating the plant requires in vitro culture technique for its mass production to conserve this endemic tree species. Plant tissue culture is an efficient technique for the conservation of endemic plant species using Murashige and Skoog (MS) [5].…”

Section: Introductionmentioning

confidence: 99%

Tissue Culture of Dillenia Philippinensis Rolfe

Lumeran¹

2016

International Advanced Research Journal in Science, Engineering

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A protocol was established for mass propagation of Dillenia philippinensis Rolfe. by tissue culture using mature seeds as initial explants inoculated in vitro on solid Knudson C with 0.5 mg/L benzyladenine (BA) and naphthalene acetic acid (NAA) (T 1 ). Root calli from the cultured seeds were grown in vitro using solid Murashige and Skoog (MS) culture medium with varying concentrations of BA and NAA. Shoot formation was high in 1.0 mg/L BA and NAA (T 2 ) at 140-175 days of culture. Microcuttings were rooted in semi-solid MS medium with different concentrations of NAA and BA. The use of NAA only in semi-solid MS medium at 0.186 mg/L initiated rooting of microcuttings. Plantlets were harvested and transferred to pots with coco coir dust for acclimatization and hardening under greenhouse condition and later grown in the field. Periodic monitoring proved 100% survival of in vitro plantlets under greenhouse and field conditions.

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“…Therefore, in order to overcome these problems, a new strategy was developed using agar-GELRITE in different combinations. Solidifying agents in the culture medium alone, or in combinations, exerted significant effects on the in vitro shoot/bulblet regeneration behavior (Tsao and Reed, 2002;Uzun et al, 2014a). Agar and GELRITE are natural polysaccharides that are mostly used at certain concentrations depending mainly on explant or plant type and culture conditions (Ebrahim and Ibrahim, 2000).…”

Section: Discussionmentioning

confidence: 99%

Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica

Çakmak¹,

Karaoğlu²,

Aasım³

et al. 2016

Turk J Biol

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IntroductionThe genus Fritillaria (Liliaceae) is an important geophytic taxon with more than 100 species and wide distribution in different climatic zones ranging from southern Europe to the Middle East (Le Nard and De Hertogh, 1993), including Turkey (Bryan, 2002). In Turkey, there are 35 species representing 48 taxa of Fritillaria, and it is ranked 4th after Allium, Iris, and Crocus. Most of the Fritillaria species in Turkey are endemic. The plants flower during spring and are widely accepted as ornamental plants due to their attractive flowers (Le Nard and De Hertogh, 1993). Besides that, the Fritillaria species are used as medicinal plants (Rønsted et al., 2005;Wang et al., 2005) in traditional Chinese medicine (Li et al., 2001) due to a wide array of alkaloids with interesting phytochemical properties.Persian lily (Fritillaria persica) is found mainly in Hatay, Mersin, and Adıyaman provinces of Turkey, as well as in Lebanon, Jordan, and Iran at 700 m to 2800 m above sea level. The bulbs are 3-5 cm in diameter with 2-3 succulent leaves. The plant has 10-25 leaves, which are approximately 15 cm long and 3 cm wide. It bears 7-20 purple bell-shaped flowers, growing in racemose position. The plant can grow up to 20-60 cm in height (Ulug et al., 2010).Conventional plant production methods currently used for the Fritillaria species are seed or bulb propagation. These propagation techniques have certain disadvantages such as very low or unpredictable germination of seeds due to physiological dormancy (Baskin and Baskin, 2004), with weak seedlings, low survival rate, and slow growth, which may take 3-4 years for bulblet maturation (Le Nard and De Hertogh, 1993). Apart from this, a limited availability of bulblets from nature (Subotić et al., 2010) also makes it difficult to get sufficient material to propagate these ornamental species. These limitations suggest the need to develop alternative propagation methods (Ulug et al., 2010) for commercial production of these valuable species.A number of regeneration protocols have been reported for different Fritillaria species by

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Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Cited by 11 publications

References 30 publications

Leaf-derived organogenesis in vitro for mass propagation of lisianthus (Eustoma grandiflorum (Raf.) Shinn

Leaf-derived organogenesis in vitro for mass propagation of lisianthus (Eustoma grandiflorum (Raf.) Shinn

Tissue Culture of Dillenia Philippinensis Rolfe

Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica

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