Effect of periodontal dressings on fibroblasts in vitro

Abstract

This study was designed to evaluate the effect of four commercially available dressings (Coe, PPC‐Eugcnol, PPC‐Non Eugenol, and Ward's Wondr Pak) on a monolayer of cells, employing the cell culture assay plate assembly. Monolayers of L929 fibroblasts were grown out on the cell culture assay plates and stained with a vital stain, .01 % Neutral Red, 8 mm discs of prepared periodontal dressings, as well as sterile filter paper and high density polyethylene discs, which served as controls, were placed on the monolayer. Observations were made at 1, 2, 6, 8 and 24 hour intervals, and measurements of the toxic zones were recorded. Results indicate that all periodontal dressings tested in this system demonstrated definite cytotoxicity. Both macroscopically and microscopically, the first signs of cell damage and/or death were observed adjacent to Ward's Wondr Pak and PPC‐Non Eugenol dressings. Although these two dressings produced the earliest toxic signs, after 24 hours Coe‐Pak produced the most extensive zones of destruction, while PPC‐Eugenol appeared to be the least toxic.

In Vitro Comparison of Biological Effects of Coe-Pak and Reso-Pac Periodontal Dressings

“…Mouse fibroblasts (3T3), L929 and HeLa cells, human epithelial cells, human gingival fibroblasts and leukocytes have been used for this purpose [7,12,35-37]. …”

Effects of periodontal dressings on fibroblasts and gingival wound healing in dogs

“…Mouse fibroblasts (L 929, 3T3 cells), Hela cells, human epithelial cells, human gingival fibroblasts and leukocytes have been used (Hildebrand and De Rensis, 1974;Rivera-Hidalgo et al, 1977;Haugen and Hensten-Pettersen, 1978;Gilbert et al, 1994;Alpar et al, 1999). Soluble material leached from some formed dressings has been found toxic to cells in culture (Hildebrand and De Rensis, 1974;Rivera-Hidalgo et al, 1977) and dilution of soluble extracts from periodontal dressings decreased this toxic effect (Eber et al, 1989;Alpar et al, 1999).…”

Effect of Soluble Extracts from Periodontal Dressings on Human Granulocytic Leukocytes in Vitro

“…Hildebrand and DeRenzis placed discs of four different formed dressings on monolayers of cultured fibroblasts. 14 Finally, Guglani and Allen studied the connective tissue reaction in rats to implants of a eugenol-contain ing and the same eugenol-free periodontal dressing used here. 7 While scored tissue reactions to each were "mild" 2 days following implantation, a "moderate" reaction to the eugenol-free dressing occurred by day 7 which was not produced with the eugenol-containing dressing.…”

“…Indeed, where fluids remain relatively stationary, cellular toxicity has been shown to be maxi mal adjacent to the dressing. 14…”