Effect of Soluble Extracts from Periodontal Dressings on Human Granulocytic Leukocytes in Vitro

Abstract

Purified populations of human polymorphonuclear granulocytic leukocytes were exposed in vitro to solubilized material(s) extracted from two commerical periodontal dressings. The composiitonof one dressing contained eugenol while that of the other lacked it. Extracts were prepared by placing each mixed dressing in isotonic saline for 18 to 24 hours. Granulocytic leukocytes received serial dilutions of each extract and were then evaluated at 15 minutes and at 3 hours of culture for changes in viability and elaboration of cyto-plasmic and lysosomal enzymes. High concentrations of extract from each dressing were toxic to the cultured granulocytes. Measurements for cell viability and release of cytoplasmic enzyme revealed that the toxic potential in the eugenol-free dressing was greater. With cell damage, extracellular release of the lysosomal enzymes was found to occur. Serially increased dilutions of either extract to nontoxic concentrations failed to selectively release lysosomal enzymes.

Effects of periodontal dressings on fibroblasts and gingival wound healing in dogs

“…Fittydent is a soluble paste which adheres to the underlying wound surface. Therefore, the cellular toxicity of such dressing in clinical situation would be much greater according to the data of Rivera-Hidalgo et al (1977). It was shown that Fittydent has rather small effect on cell proliferation (75.1 ± 14.3% of control after 24 h and 71.6 ± 8.7 of control after 48 h) but it exerted a drastic effect on cell survival (no cell colony was formed after the treatment).…”

“…The study with the soluble extracts from periodontal dressings on leukocytes in vitro showed that a 10-fold dilution of extract decreased their toxic effect (Rivera-Hidalgo et al, 1977). The present results, where the cytotoxic effects of Peripac, Barricaid, Fittydent, and ResoPack periodontal dressings and Myzotect-tincture were tested on cell proliferation and cell survival of fibroblast V-79 cell line in vitro, indicate that Barricaid showed the most favourable results, followed by Reso-Pack, Peripac, Fittydent, and Myzotect-tincture.…”

“…Mouse fibroblasts (L 929, 3T3 cells), Hela cells, human epithelial cells, human gingival fibroblasts and leukocytes have been used (Hildebrand and De Rensis, 1974;Rivera-Hidalgo et al, 1977;Haugen and Hensten-Pettersen, 1978;Gilbert et al, 1994;Alpar et al, 1999). Soluble material leached from some formed dressings has been found toxic to cells in culture (Hildebrand and De Rensis, 1974;Rivera-Hidalgo et al, 1977) and dilution of soluble extracts from periodontal dressings decreased this toxic effect (Eber et al, 1989;Alpar et al, 1999).…”

“…If the dressing adheres to the underlying wound tissue, high concentrations of toxic material are solubilised in the relatively stationary tissue fluids at the pack-tissue junction. Saliva flow cannot dilute soluble toxic substances, so the cellular toxicity of such dressings would be enhanced adjacent to the dressing (Rivera-Hidalgo et al, 1977).…”

The protective effect of zinc on rosin and resin acid toxicity in human polymorphonuclear leukocytes and human gingival fibroblastsin vitro

“…[8][9][10]30 Oleoresin and eugenol also are components of several in which their the antibacterial activities were investigated. 45 Evidence that resin acids and oleoresins are dental materials and wound dressings that have been shown to have cytotoxic effects in vitro and on HeLa released from different materials used in vivo has been shown indirectly 36,46,47 although it is not known to what cells, 31 polymorphonuclear leukocytes, 32 and fibroblasts [33][34][35] and in vivo. 5,8,9,36 extent.…”