Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures

Pasqualotto, Eleonora Bedin; Agarwal, Ashok; Sharma, Rakesh K.; Izzo, Vicente Mário; Pinotti, José Aristodemo; Joshi, Narendra; Rose, Bruce I.

doi:10.1016/j.fertnstert.2003.11.021

Cited by 221 publications

(188 citation statements)

References 11 publications

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“…Regarding to the effect of ALA on developmental competence of isolated follicles, there is no information available. Some reports have suggested that a relationship between redox-state of the follicular fluid and oocyte quality in which levels of TAC and ROS can be used as a predictive sign of successful IVF [44]. This is in consistent with our finding that showed presence of 100 uM of ALA in maturation medium results in decrease ROS production and increase TAC level which in turn lead to improve maturation and developmental competence of isolated follicles.…”

Section: Discussionsupporting

confidence: 93%

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The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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Purpose This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue. Methods Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α-minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively. Results In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p<0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p<0.05). Conclusion ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.

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Section: Discussionsupporting

confidence: 93%

“…In this sense oxidative stress (OS) has been recently implicated as one of the most effective factors [3,7,44,56]. Critical levels of reactive oxygen species (ROS) are necessary for many biochemical pathways involve in physiological functions.…”

Section: Introductionmentioning

confidence: 99%

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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“…This may contribute for improving the reproduction. Indeed, there is a good correlation between superoxide dismutase and catalase levels in follicular fluid of women, and fertilisation and cleavage rates (Pasqualotto et al, 2004). Supplementation with antioxidants before the beginning of months of heat-stress and also during the stress period may correct the infertility due to heat-stress through decreased cortisol secretion and oxidative stress, resulting in enhanced pregnancy rates (Megahed et al, 2008).…”

Section: Fas Antioxidants and Reproductive Performancementioning

confidence: 99%

Nutritional sub-fertility in the dairy cow: towards improved reproductive management through a better biological understanding

Friggens

Disenhaus

Petit

2010

Animal

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There has been a significant decline in the reproductive performance of dairy cattle in recent decades. Cows, take longer time to return to the oestrus after calving, have poorer conception rates, and show fewer signs of oestrus. Achieving good reproductive performance is an increasing challenge for the dairy producer. In this study we focus on understanding the overall biological phenomena associated with nutritional sub-fertility rather than the underlying multiplicity of physiological interactions (already described in a number of recent studies). These phenomena are important because they represent the natural adaptations of the animal for dealing with variations in the nutritional environment. They can also be used to monitor and modulate reproductive performance on-farm. There is an underlying trade-off between two aspects of reproduction: investment in the viability of the current calf and investment in future offspring. As the investment in, and viability of, the current calf is related to maternal milk production, we can expect that level of milk production per se has effects on subsequent reproductive performance (investment in future offspring). Lactating cows have a lower proportion of viable embryos, which are of poorer quality, than do non-lactating cows. The same applies to high-compared to medium-genetic merit cows. Another important biological property is the adaptive use of body reserves in support of reproduction. Orchestrated endocrine changes in pregnancy and lactation facilitate the deposition of body lipid during pregnancy and mobilisation in early lactation. When the cow fails to accumulate the reserves she needs to safeguard reproduction she delays committing to further reproductive investment. But how does the cow 'know' that she is failing in energy terms? We argue that the cow does this by 'monitoring' both the body fat mobilisation and body fatness. Excessive body fat mobilisation indicates that current conditions are worse than expected. Body fatness indicates the future ability of the cow to safeguard her reproductive investment is compromised. Both delay further reproductive commitment. The relationship between reproductive performance and; milk production as an index of maternal investment, body fatness as an index of ability to safeguard reproductive investment, and body fat mobilisation as an index of the current nutritional environment -are examined. Nutritional strategies that seek to modulate body mobilisation and the endocrine environment by use of glucogenic and lipogenic diets, and the use of in-line progesterone profiles to monitor reproductive status are then discussed in this biological context.

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“…The role of reactive oxygen species (ROS) in gynecological diseases and assisted reproduction has been widely studied in recent years [6][7][8][9][10][11]. Low ROS concentrations in the follicular fluid have been proposed to be important for successful IVF outcome [12].…”

Section: Introductionmentioning

confidence: 99%

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar

Kopitar

Ihan

et al. 2007

J Assist Reprod Genet

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Purpose: The aim was to establish the impact of human granulosa cell apoptosis and reactive oxygen species (ROS) production on fertilization competence of the oocyte, embryo developmental stage and implantation rate.Methods: Thirty women undergoing IVF-ET for tubal factor infertility were included; GnRH antagonists and gonadotrophins were used for ovarian stimulation. Granulosa cells were isolated from each aspirated follicle using gradient centrifugation. Apoptosis was studied by flow cytometry using annexin V and propidium iodide. ROS production was studied with hydroethidine staining and analyzed by flow cytometry.Results: There were no differences in characteristics of granulosa cells between the follicles with fertilized and nonfertilized oocytes. The analyzed characteristics of granulosa cells in corresponding follicles had no effect on embryo developmental stage on day 5. The percentage of ROS producing granulosa cells was lower in the follicles giving rise to blastocysts that resulted in implantation compared to those that did not (39.9% versus 69.9%, P = 0.031).Conclusions: Apoptosis and ROS production in granulosa cells have no significant impact on fertilization and do not correlate with the development of blastocysts. An increased

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Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures

Cited by 221 publications

References 11 publications

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Nutritional sub-fertility in the dairy cow: towards improved reproductive management through a better biological understanding

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

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