The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Abstract

Purpose This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue. Methods Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α-minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively. Results In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p<0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p<0.05). Conclusion ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.

Antioxidant properties of coenzyme Q10‐pretreated mouse pre‐antral follicles derived from vitrified ovaries

“…In the present study, TAC in the cultured pre‐antral follicles decreased until 96 h in both vitrified and fresh groups. This is consistent with a prior observation . The TAC level during in vitro culture is the main benchmark of the quality of cultured follicles because a reduction in TAC can result in OS .…”

Insights into Mechanisms Causing the Maternal Age-Induced Decrease in Oocyte Quality

“…However, care must be taken to avoid overcompensation of antioxidant supplementation. Evidence from mice showed the antioxidant alpha lipoic acid (ALA) exposure in high concentrations was detrimental to follicular development, oocyte maturation, and preimplantation development despite benefi ts at lower concentrations [ 71 ]. This study demonstrates that antioxidant supplementation can be benefi cial, but also that ROS are a necessary component of oocyte maturation and conception, so dosage must be monitored and controlled.…”

THE EFFECT OF ALPHA LIPOIC ACID ON BLASTOCYST EMBRYO DEVELOPMENT IN VIVO IN MICE (Mus musculus) EXPOSED CIGARETTE SMOKE

“…ALA dengan dosis 100 uM memiliki rata-rata perkembangan folikel, oosit, dan perkembangan embrio lebih tinggi dibandingkan kelompok lainnya serta dapat menurunkan ROS dan meningkatkan total antioxidant capacity (TAC) setelah dilakukan kultur selama 96 jam (Talebi et al, 2012). Hasil penelitian ini menunjukkan bahwa ALA memiliki peran yang baik dalam proses oksidatif piruvat dalam mempersiapkan sumber energi yang dibutuhkan selama perkembangan embrio (Harris, 2002;Johnson et al, 2007).…”

“…Pada kondisi in vivo, antioksidan enzimatik maupun non enzimatik in vitro memiliki proteksi yang baik untuk stres oksidatif yang disebabkan perubahan patologis dan mempertahankan kadar optimal dari ROS. Sedangkan pada kondisi tingginya kadar oksigen dan kurangnya mekanisme pertahanan secara fisiologis terhadap ROS akan mengakibatkan stres oksidatif (Talebi et al, 2012;Gupta et al, 2010). Glutathione merupakan antioksidan endogen yang memiliki fungsi untuk detoksifikasi dan eliminasi berbagai macam karsinogen dan toksin, juga berfungsi dalam sistem pertahanan dengan cara memotong reaksi oksidasi berantai radikal bebas atau dengan menangkapnya sehingga dapat mencegah terjadinya stres oksidatif (Winarsi, 2007).…”