Effect of ouabain, digoxin and digitoxigenin on potassium uptake and histamine release from rat peritoneal mast cells

Abstract

Rat peritoneal mast cells were used to study the effects of digitalis glycosides on potassium uptake and histamine release induced by compound 48/80, substance P and egg-albumin (immunological release). In the absence of calcium all glycosides inhibited potassium uptake. Ouabain and digoxin enhanced the histamine release while digitoxigenin either had no effect or was slightly inhibitory. In the presence of calcium, the glycosides only affected potassium uptake and histamine release slightly. In the presence of lithium or lanthanum the enhancement of the histamine release was counteracted. Hydrophilic digitalis glycosides seem to enhance histamine release secondary to an increase in intracellular sodium. Lipophilic glycosides have no effect on the release.

Signaling pathway in nerve growth factor induced histamine release from rat mast cells

“…The collected cells were resuspended in buffer-salt solution and centrifuged at 220 g for 10 min. For preparation of purifi ed mast cells, the cells were transferred to a HEPES-buffered (32 mmol/l) Percoll solution (1017 g/ml) [34]. A gradient of Percoll was created by centrifugation at 21 000 g for 30 min at 4 °C.…”

Direct thrombin inhibitors built on the azaphenylalanine scaffold provoke degranulation of mast cells

“…The collected cells were re-suspended in modified Krebs-Ringer solution and centrifuged at 220gfor 10 min. For preparation of purified mast cells,the cells were transferred to a HEPES-buffered(32 mM) Percollsolution (1.017g/ml) (23).A gradient of Percollwas created by centrifugation at 21000gfor 30 min at 4°C.After centrifugation,mastcells were located in the lowerthird of the gradient.Percoll wasremoved by washing the mast cellfraction with modifiedKrebs-Ringersolution and by additionalcentrifugation (twice at 220g for 10 min) of the fraction containing mast cells. Ap urity of mast cells after the isolation wasmore than 98% (23).…”

“…Cells were suspended in modifiedK rebs-Ringers olution and aliquots transferred to plastic tubestoattain afinalvolume of 0.6 ml, each containing about 2´1 0 5 mast cells (23). Cells were stimulatedwith LK-732(from 5.8 µ Mto580 µ M), compound 48/80 (1 µ gml -1 )orotherinvestigated direct thrombin inhibitors (200 µ M) and the incubation wasallowedtoproceed for 15 min at 37 o C. Thesecretion of mast celldegranulation marker histamine (24)w as stoppedb yi mmersing the tubesi na ni ce-cold bath and adding ice-cold modifiedK rebs-Ringers olution (1.5 ml).…”

Regulatory role of extracellular Na + and Ca 2+ ions in nerve growth factor induced histamine secretion from rat mast cells

“…The collected cells were resuspended in buffersalt solution and centrifuged at 220 g for 10 min. For preparation of purified mast cells, the cells were transferred to a HEPES-buffered (32 mmol/l) Percoll solution (1017 g/ml) [27]. A gradient of Percoll was created by centrifugation at 10000 g for 30 min at 4∞C.…”