Effect of nutrition of maturation of alfalfa (Medicago sativa L.) somatic embryos

Micropropagation of mother plants of lucerne (Medicago sativa L.) for somatic embryogenesis

“…bud and internode culture in subcultures 6 and 7) they were smaller than the average, confirming previous results about nutrient needs for embryoid growth (Lai & McKersie, 1993) . Experiment 3.…”

“…A 30-day subculture routine (line A) was compared to a shorter 20-day one (line B) for embryo production . At the end of each subculture of both lines A and B, petioles were excised from the first three apical expanded leaves of randomly collected shoots ; the petioles were cut into 5-mm long fragments and used for embryogenesis according to McKersie & Bowley (1993) and further refinements (Lai & McKersie, 1993 ;Shetty & McKersie, 1993) . Briefly, 10 petiole fragments were put on 20 ml of sterile agarized callogenetic medium in 90-mm Petri dishes ( , where the creamy filtrate was spread homogeneously all over the surface, in order to have a very thin layer of cell aggregates on the medium .…”

Treatments affecting maturation and germination of American chestnut somatic embryos

“…Lai and McKersie (1993) increased conversion rates of alfalfa somatic embryos with the addition of 50 mM autoclaved glutamine. Asparagine and aspartic acid produced the same increase in vigor, germination, and conversion (Lai and McKersie, 1994b).…”

“…The addition of amino acids to maturation media has also improved somatic embryo development, germination, and conversion (Finer et al, 1989;Lai and McKersie, 1993;Khlifi and Tremblay, 1995;Rao et al, 1995).…”

Influence of permafrost microorganisms on morphophysiological indicators of spring wheat

“…Они переносят электроны в возбужденном состоянии к фотохимическим реакционным центрам, по-глощая свет в той области спектра, в которой не способен поглощать хлорофилл (Зотикова и др., 2001;MacFarlena, 2002), и защищают пигменты и ненасыщенные жирные кислоты липидов от окислительного повреждения, устра-няя избыток активных форм кислорода (Lai, McKersie, 1993;Седых, Игнатьев, 2001). Увеличенное относительно хлорофиллов содержание каротиноидов может свиде-тельствовать о повышении уровня стрессоустойчивости растений.…”