Abstract:A reliable and standard method was established for micropropagation of the A70-34 selected genotype of lucerne (Medicago sativa L ., genotype A70-34), aimed at reducing contamination problems, seasonal and phenological influences on regeneration and phytosanitary problems of the mother plants, while maintaining the regenerative potential for somatic embryogenesis of the plant tissues . Mother plants were routinely maintained for several subcultures and somatic embryogenesis was regularly obtained from the subc… Show more
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