Recent Perspectives on Synthetic Seed Technology Using Nonembryogenic<i>In Vitro</i>–Derived Explants

Standardi, A.; Piccioni, Emanuele

doi:10.1086/314087

Cited by 24 publications

(12 citation statements)

References 60 publications

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“…(Singh et al 2006) using cold storage. The most important feature of the encapsulated propagules is their capability to retain viability in terms of regrowth abilities after encapsulation and even after storage (Standardi and Piccioni 1998). In this study, the encapsulated shoot cultures could be effectively stored at 25±2°C for 75 d when 16/8-h light/dark conditions were provided (Table 5).…”

Section: Resultsmentioning

confidence: 97%

In vitro approaches for conservation of Asparagus racemosus Willd.

Thakur

Tiwari

Jadhav

2015

In Vitro Cell.Dev.Biol.-Plant

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Asparagus racemosus is a commercially important medicinal plant, traditionally used for combating gynecological problems in India. The majority of plants used by the pharmaceutical industry come from wild sources, endangering the natural population of the species. The plants are being overharvested, so this species faces a real danger of becoming vulnerable in its natural habitat. Ex situ conservation using in vitro tools is a possible solution to this problem. Ex situ conservation of plants involving in vitro tools has been initiated through axillary branching using nodal explants. Studies on in vitro storage under slow-growth conditions were carried out to develop an efficient protocol for conservation of A. racemosus germplasm. In vitro shoot cultures generally require a 4-wk subculture onto fresh medium when grown at 25±2°C under a 16-h photoperiod. In this research, the use of mannitol or sorbitol as an osmoticum and reduction of sucrose to 1.5% (w/v) in half-strength MS medium led to maintenance of the cultures for 6 mo at 25±2°C with no subculture. Surviving shoots from the slow-growth cultures could be regenerated with 100% efficiency, indicating that the subculture interval was successfully extended by this method. Temperature and medium modification both had significant effects on the growth of stored shoots, and the two factors showed significant interaction. In experiments designed to test encapsulation as a storage method, micropropagated shoot clusters encapsulated in calcium alginate beads were successfully stored up to 75 d at 25±2°C under a 16-h photoperiod. Stored shoots from both storage methods were subsequently recovered and multiplied on MS medium with 3% sucrose and 1.11 μM benzylaminopurine at 25 ± 2°C. Welldeveloped shoots were rooted and acclimatized successfully.

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Section: Resultsmentioning

confidence: 97%

In vitro approaches for conservation of Asparagus racemosus Willd.

Thakur

Tiwari

Jadhav

2015

In Vitro Cell.Dev.Biol.-Plant

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“…The encapsulation of nonembryogenic in vitro-derived explants in Na-Ca alginate beads could be useful tool both for transfer of aseptic material between laboratories and for storage of valuable germplasm (Standardi and Piccioni 1998). In realizing the idea of providing an 'artificial endosperm', the nutritive ingredients of the alginate beads are of key importance for both the storage and conversion efficiencies of the propagules encapsulated.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Effect of alginate matrix composition on regrowth of in vitro-derived encapsulated apical microcuttings of hybrid aspen

Tsvetkov¹,

Jouve²,

Hausman³

2006

Biol. plant.

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Effect of alginate matrix composition on regrowth performance of encapsulated microcuttings of hybrid aspen (Populus tremula L. × P. tremuloides Mincx.) was studied. Both high regrowth frequency and viability of explants were registered in all encapsulation mixtures tested. Some ingredients of the matrix (nutrient medium salts, sugars, growth regulators) significantly affected the initial development of the microcuttings. Sucrose appeared to play an important role in the starting stage of the regrowth event.Additional key words: encapsulation, propagules, Populus sp. ⎯⎯⎯⎯Despite of being basic alternative for production of 'synthetic seeds' (Redenbaugh et al. 1986, Attree and Fowke 1993, Uozumi and Kobayashi 1995, the encapsulation technique offers attractive opportunities for supplementary applications. The encapsulation of nonembryogenic in vitro-derived explants in Na-Ca alginate beads could be useful tool both for transfer of aseptic material between laboratories and for storage of valuable germplasm (Standardi and Piccioni 1998). In realizing the idea of providing an 'artificial endosperm', the nutritive ingredients of the alginate beads are of key importance for both the storage and conversion efficiencies of the propagules encapsulated. Effects of various compositions of sodium alginate matrix on regrowth performance of encapsulated apical cuttings of hybrid aspen were examined in a pilot study.Shoot apical microcuttings 0.5 -0.7 cm in length isolated from in vitro proliferating clone of Populus tremula L. × P. tremuloides Mincx. were used in the experiment. Microcuttings were encapsulated in accordance with the following procedure. For coating with sodium alginate, explants were immersed in sterilized encapsulation mixtures with different composition: a) distilled water + 4 % sodium alginate (W); b) distilled water + 3 % sucrose + 4 % sodium alginate (W+S); c) Murashige and Skoog (1962, MS) nutrient medium + 4 % sodium alginate (M); d) MS medium + 1.3 μM 6-benzylaminopurine (BAP) + 4 % sodium alginate (M+B); e) MS medium + 3 % sucrose + 4 % sodium alginate (M+S); f) MS medium + 1.3 μM BAP + 3 % sucrose + 4 % sodium alginate (M+B+S). The alginate-covered microcuttings were dropped into stirred 1.4 % CaCl 2 solution and kept 5 min for complexation and formation of capsules. The capsules were rinsed thrice 1 min each time with liquid MS medium supplemented with 1.3 μM BAP and 3 % sucrose.Encapsulated microcuttings were transferred for regeneration on MS medium supplemented with 1.3 μM BAP, 3 % sucrose and solidified with 0.8 % agar. Glass jars (300 cm 3 ) with 50 cm 3 nutrient medium were used for all experimental treatments. Each variant consisted of five jars, with five capsules being placed into each of them. Non-encapsulated microcuttings were directly placed on the same medium as a control. The jars were kept in a cultivation room at 24 ± 1 ºC, 16-h photoperiod and irradiance of 40 μmol m -2 s -1 provided by cool-white fluorescent lamps (GroLux ™ , Sylvania, Raunheim, Germany).After period of four weeks...

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“…Encapsulation technology has recently been attracting the interest of researchers in the field of plant propagation because it combines the advantages of zygotic or gamic seeds with those of micropropagation (such as high efficiency of propagation, reduced space requirement, ease of handling and transportability, storability, reduced size of the propagules and automation potentiality). As such, it represents a new and powerful tool in the plant nursery field as well as in approaches to germplasm conservation and plant material exchange (Standardi and Piccioni 1998;Micheli et al 2003). Specifically, synthetic seed or artificial seed (or synseed), described as ''artificially encapsulated somatic embryos, shoots or other tissues which can be used for sowing under in vitro or ex vitro conditions'' (Aitken-Christie et al 1995), is the main product of this technology.…”

Section: Introductionmentioning

confidence: 99%