Organogenesis and encapsulation of in vitro-derived propagules of Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirius trifoliata (L.) Raf]

Germanà, María Antonietta; Micheli, Maurizio; Chiancone, Benedetta; Macaluso, Laura; Standardi, A.

doi:10.1007/s11240-011-9921-y

Cited by 49 publications

(14 citation statements)

References 48 publications

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“…Regeneration through somatic embryogenesis is rather common in different Citrus species, and it is also valuable for clonal propagation, synthetic seed production, and germplasm storage (Germanà 2005;Germanà et al 2011).…”

Section: Characterization Of Regenerants: Ploidy Analysis and Analysimentioning

confidence: 99%

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Cardoso

Abdelgalel

Chiancone

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

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In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the genotype: both clementines, namely Hernandina and Corsica, produced homozygous and triploid regenerants (microspore-derived embryos), whereas all of the analyzed regenerants from sweet oranges, three cultivars of Tarocco and Moro, produced heterozygous and diploid regenerants similar to the parental genotypes (somatic embryos).

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Section: Characterization Of Regenerants: Ploidy Analysis and Analysimentioning

confidence: 99%

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Cardoso

Abdelgalel

Chiancone

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

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“…The technique of synseed has been widely studied and works with various plant species including fruits, cereals, medicinal plants, vegetables, ornamentals, forest trees, and orchids (Ballester et al 1997;Gonzalez-Benito et al 1997;Standardi and Piccioni 1998;Ara et al 2000;Nyende et al 2003;Chand and Singh 2004;Tsvetkov and Hausman 2005;Malabadi and Staden 2005;Naik and Chand 2006;Faisal et al 2006;Faisal and Anis 2007;Antonietta et al 2007;Micheli et al 2007;Pintos et al 2008;Rai et al 2008;Sundararaj et al 2010;Singh et al 2010;Germana et al 2011;Mishra et al 2011). In addition, during cold storage, encapsulated nodal segments require no transfer to fresh medium, thus reducing the cost of maintaining germplasm cultures (West et al 2006).…”

Section: Advancement In Plant Tissuementioning

confidence: 99%

Review of Literature

Varshney¹,

Anis²

2014

Trees: Propagation and Conservation

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Gottlieb Haberlandt (Math Naturwiss 111:69-92), a German plant physiologist, for the first time initiated the work on tissue culture. His work arose as a research tool and attempts were made to culture the isolated, fully differentiated cells in nutrient medium in vitro as early as 1898. The theoretical basis of tissue culture lies in the cell theory given by Schleiden and Schwann (1838-1839). Practically, this technique stands on the concept of "totipotency," i.e., each cell has the ability to regenerate into a new plant. The field finds a wide range of applications starting from mass clonal propagation to plant improvement, molecular biology, bio-processing as well as a basic research tool. It has advanced the production in forestry and agriculture to many folds. There are a number of reviews published on tissue culture of woody and tree species which provide the wide-ranging micropropagation reports of various plant species. In this way, this chapter highlights the current review in tree tissue culture in vitro and micropropagation of other valuable plants, their significance, and the wide scope existing for investigations on mass multiplication and conservation of these plants.

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“…Pretreatment of explants with cytokinin(s) and auxin(s) has enhanced the conversion frequency of synseeds into plantlets for the following: mulberry (Malus pumilla) (Pattnaik et al, 1995); pineapple (Ananas comosus) (Soneji et al, 2002); Dalbergia sissoo (Chand and Singh, 2004); Carrizo citrange (Germanà et al, 2011); Corymbia spp. and African mahogany (Hung and Trueman, 2012).…”

Section: Introductionmentioning

confidence: 99%

Production of Synseed for Hybrid Cymbidium Using Protocorm-Like Bodies

Silva

2012

Journal of Fruit and Ornamental Plant Research

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A B S T R A C TSynthetic seed were produced from protocorm-like bodies (PLBs) of hybrid Cymbidium Twilight Moon 'Day Light' after culture on a new medium, Teixeira Cymbidium (TC) medium. This new medium contained, in addition to a unique selection of macro-and micronutrients, 0.1 mg/l α-naphthaleneacetic acid and 0.1 mg/l kinetin, 2 g/l tryptone and 20 g/l sucrose, and was solidified with 8 g/l Bacto agar. Several explant types and sizes (intact PLBs, half-PLBs, PLB longitudinal thin cell layers) were tested. In addition, pretreatment of PLB-synseeds with 200 mM KNO 3 solution, the addition of activated charcoal or coconut water to synseeds, light vs dark culture, short-term (1 month) and long-term (6 and 12 months) low-temperature (4°C) storage, as well as cryostorage were also tested. All treatments resulted in less PLBs than the control treatment. Among all these treatments, only the use of TC medium or incorporation of coconut water into synseeds resulted in "germination" while lowtemperature storage (1-6 months) was only possible under liquid TC. These results would allow for the short-term preservation of Cymbidium germplasm but not for effective cryopreservation.

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Organogenesis and encapsulation of in vitro-derived propagules of Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirius trifoliata (L.) Raf]

Cited by 49 publications

References 48 publications

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Review of Literature

Production of Synseed for Hybrid Cymbidium Using Protocorm-Like Bodies

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