Effect of Nonsurgical Periodontal Therapy on Crevicular Fluid and Serum Glutathione Peroxidase Levels

Abstract

Abstract.Background: Plasma glutathione peroxidase (eGPx) is an important selenium containing antioxidant in human defense against oxidative stress. While crevicular fluid (GCF) eGPx levels and its association with periodontal disease is well documented, there is no data on correlation of GCF and serum eGPx levels in chronic periodontitis. Hence this study was undertaken to further probe into the role of oxidative stress in periodontal diseases and effect of nonsurgical periodontal therapy (NSPT) by correlating GCF and serum levels of eGPx. Materials and methods: Thirty subjects (16-Males and 14-Females; age: 30-38 years) participated in the study. The subjects were divided, based on gingival index, probing pocket depth and clinical attachment level into: Healthy (group-1, n = 10), Gingivitis (group-2, n = 10) and Periodontitis (group-3, n = 10). Chronic periodontitis patients after NSPT constituted group 4. GCF and serum samples collected from each subject were quantified for eGPx levels using Enzyme linked Immunosorbent Assay. Results: The mean eGPx concentrations increased from health (14.01 ng/µl and 78.26 ng/ml) to gingivitis (22.86 ng/µl and 90.44 ng/ml) and then to periodontitis (29.89 ng/µl and 103.43 ng/ml), in GCF and serum respectively. After NSPT, there was statistically significant reduction in eGPx concentration in GCF and serum (19.41 ng/µl and 85.21 ng/ml). Further, all the GCF eGPx values showed a positive correlation to that of serum eGPx level. Conclusion: Thus, increased eGPx concentration in GCF can be considered as an indicator of local increase in oxidative stress. While, increase in serum eGPx levels indicates that periodontal disease can also lead to increased oxidative stress at the systemic level.

The effect of periodontal therapy on oxidative stress biomarkers: A systematic review

“…The search strategy yielded 3,199 studies, but just 20 fulfilled the inclusion criteria (Akpinar, Toker, Ozdemir, Bostanci, & Aydin, ; Bostanci, Toker, Senel, Ozdemir, & Aydin, ; Chaudhary, Gowda, Mehta, & Kumar, ; Cifcibasi et al., ; Dede, Ozden, & Avcı, ; Grant, Brock, Matthews, & Chapple, ; Guentsch et al., ; Hendek, Erdemir, Kisa, & Ozcan, ; Koromantzos et al., ; Kurgan et al., ; Marcaccini et al., ; Mizuno et al., ; Muthuraj et al., ; Novakovic et al., ; Önder et al., ; Patel, Rao, & Pradeep, ; Singh, Chander Narula, Kumar Sharma, Tewari, & Kumar Sehgal, ; Tamaki, Tomofuji, Ekuni, Yamanaka, & Morita, ; Toker, Akpınar, Aydın, & Poyraz, ; Wei, Zhang, Wang, Yang, & Chen, ). Figure summarizes the flow chart of the inclusion process, and the reasons for exclusions are expressed in Supporting Information Table .…”

“…Figure summarizes the flow chart of the inclusion process, and the reasons for exclusions are expressed in Supporting Information Table . The majority of the included studies were non‐RCTs (Akpinar et al., ; Bostanci et al., ; Cifcibasi et al., ; Dede et al., ; Grant et al., ; Guentsch et al., ; Hendek et al., ; Kurgan et al., ; Marcaccini et al., ; Muthuraj et al., ; Önder et al., ; Patel et al., ; Singh et al., ; Tamaki et al., ; Toker et al., ; Wei et al., ). Four studies were RCTs (Chaudhary et al., ; Koromantzos et al., ; Mizuno et al., ; Novakovic et al., ).…”

Evaluation of the effects of ascorbic acid on azathioprine-induced alteration in the testes of adult Wistar rats

“…Elevated level of GPx is an indicator of testicular oxidative damage (Patel et al, 2012). This could be responsible for the changes observed in the testicular histology of the treated animals, with progressive decline in the population and size of the developing spermatogenic cells and the mature sperm cells.…”

Resistin in serum and gingival crevicular fluid as a marker of periodontal inflammation and its correlation with single-nucleotide polymorphism in human resistin gene at −420

“…The resistin levels in GCF was found to be remarkably higher than those in serum, these findings were similar to those reported before in Japanese population[15] and is in accordance to various other inflammatory mediators in periodontal diseases. [2331]…”

“…[212223] Briefly, all clinical assessments using a periodontal probe (UNC-15, Hu-Freidy, Chicago, IL, USA), radiographs, group allocations and sampling-site selections were performed by one examiner (ARP) to ensure adequate intra-examiner reproducibility. Samples were collected on the subsequent day by a second examiner (SPP).…”