Effect of Multilaminate Small Intestinal Submucosa as a Barrier Membrane on Bone Formation in a Rabbit Mandible Defect Model

Abstract

A barrier membrane (BM) is essential for guided bone regeneration (GBR) procedures. Absorbable BMs based on collagen have been widely applied clinically due to their excellent biocompatibility. The extracellular matrix (ECM) provides certain advantages that can compensate for the rapid degradation and insufficient mechanical strength of pure collagen membrane due to the porous scaffold structure. Recently, small intestinal submucosa (SIS), one of the most widely used ECM materials, has drawn much attention in bone tissue engineering. In this study, we adopted multilaminate SIS (mSIS) as a BM and evaluated its in vivo and in vitro properties. mSIS exhibited a multilaminate structure with a smooth upper surface and a significantly coarser bottom layer according to microscopic observation. Tensile strength was 13.10 ± 2.56 MPa. In in vivo experiments, we selected a rabbit mandibular defect model and subcutaneous implantation to compare osteogenesis and biodegradation properties with one of the most commonly used commercial collagen membranes. mSIS was retained for up to 3 months and demonstrated longer biodegradation time than commercial collagen membrane. Quantification of bone regeneration revealed significant differences in each group. Micro-computed tomography (micro-CT) revealed that the quantity and maturity of bones in the mSIS group were significantly higher than those in the blank control group (P < 0.05) and were similar to those in a commercial collagen membrane group (P > 0.05) at 4 and 12 weeks after surgery. Hematoxylin and eosin staining revealed large amounts of mature lamellar bone at 12 weeks in mSIS and commercial collagen membrane groups. Therefore, we conclude that mSIS has potential as a future biocompatible BM in GBR procedures.

Bone Augmentation of Peri-Implant Dehiscence Defects Using Multilaminated Small Intestinal Submucosa as a Barrier Membrane: An Experimental Study in Dogs

“…In a rat cranial defect model, significant new bone formation can be observed in defect areas covered by the SIS membrane, particularly in areas close to the membrane [16]. Similar results have been achieved using mSIS combined with the bone substitute material in mandibular bone defects in a rabbit model [17]. Here, we compared the mSIS membrane with widely used collagen membranes for GBR in beagle implant models utilizing clinical methods.…”

“…e mSIS comprised eight layers of acellular monolayer porcine SIS, produced as a lyophilized stack. e manufacturing process is described in a previous paper [17].…”

“…Moreover, SIS has demonstrated osteoinductive potential for use in the GBR procedure [16]. e multilaminated small intestinal submucosa (mSIS) membrane was used in this study, as it was shown to exhibit higher mechanical strength in a prior preclinical study [17]. e use of mSIS with enhanced mechanical strength, combined with the bone substitute material in the GBR procedure, significantly promoted new bone formation in bone defects [17], which suggests that the mSIS membrane may be useful as a GBR barrier membrane in clinical applications.…”

Tissue engineering applications in otolaryngology—The state of translation

“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate craniofacial tissue engineering 49‐72,74‐77,79,81‐84,145‐197 …”

Evaluating the Bone Regeneration of nHAp/PPC Membrane in Rabbit Calvarial Defect

“…The large values of Tb.Th and Tb.N indicate the maturity and stability of the bone structure. By contrast, if the value of Tb.Sp, which represents the degree of connectivity of the trabecular bone, is large, the arrangement of the bone structure is poor 23) . It was found that the Tb.N and Tb.Th of the three groups were higher than those of the control group from Table 2 at postoperative weeks 4 and 12.…”