Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture

Abstract

This study showed that mitomycin C was efficient in inhibiting GM-CSF synthesis with reduction of IL-5 secretion, but this fact needs complementary studies.

Impact of Mitomycin C on the mRNA Expression Signatures of Immunological Biomarkers in Eosinophilic Nasal Polyposis

“…12 Under these pathological conditions, mitomycin C acts as an antineoplastic and antibiotic agent because of its ability to alkylate DNA, which then prevents replication, transcription, and protein synthesis. [13][14][15] The precise mechanism by which treatment with mitomycin C complements the surgical procedure in ENP patients remains to be elucidated. It is possible that the topical application of mitomycin C minimizes the recurrence of nasal polyps after ENP surgical treatment because of its ability to inhibit fibroblast proliferation and to induce apoptosis in eosinophils, 10,14,16 which contributes to the control of the immunologic microenvironment that sustains the eosinophilic inflammation.…”

Azithromycin for the Treatment of Eosinophilic Nasal Polyposis: Clinical and Histologic Analysis

“…In the present study, tissue eosinophilia was evaluated according to the literature 18,20,21 by a doubleblinded pathologist (D.C.R.). However, at the end of the study, the investigators evaluated the samples and observed a noticeable difference in the tissues before and after treatment.…”

“…To evaluate the percentage of eosinophils, the field with the largest inflammatory infiltrate (more representative) among the five captured fields was chosen, and 100 leukocytes were counted in each sample (before and after treatment) by using ϫ400 magnification. 20,21 We stained the slides with Cromotrope 2R to better identify the eosinophils and to confirm the observations. By using the ImageJ software, the absolute number of eosinophils was counted in five captured fields in slides stained with Cromotrope 2R.…”

Topical mitomycin C application post endoscopic removal of antrochoanal polyp

“…It is used intravenously to treat upper gastro‐intestinal cancers; however its most common usage is topically and both human and animal applications confirm the safety of topical use of MMC 8 . It acts as an alkylating agent, inhibiting DNA, protein synthesis and fibroblasts in cell cultures 9 …”