Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice

Jeong, Gi Hee; Boisvert, William A.; Xi, Mei-Zhu; Zhang, Yilin; Choi, Young-Bin; Cho, Sunghun; Lee, Sang‐Hyun; Choi, Changsun

doi:10.1007/s11655-017-2755-7

Cited by 11 publications

(12 citation statements)

References 20 publications

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“…Both Geranium sibiricum extract and Miscanthus sinensis var. purpurascens extract led to cell proliferation rates below 85% at 156.3 and 62.5 ppm [24], respectively, and were, therefore, considered cytotoxic. Similarly, a Salvia plebeia extract substantially reduced cell proliferation rates at 125 ppm [26].…”

Section: Discussionmentioning

confidence: 99%

“…After 20 min in the dark at room temperature, the absorbance of the mixture was measured at 517 nm using a 7315 UV spectrophotometer (JENWAY, Staffordshire, United Kingdom). Electron-donating ability (EDA) was calculated as described as follows: , and 250 ppm), and the cells were allowed to grow in 10 μl of each NS concentration for 24 h, as described in several previous hair loss studies [10,23,24]. DMEM medium, minoxidil, and triton X-100 (Sigma) were used as a negative control (NC), positive control (PC), and blank, respectively.…”

Section: Antioxidant Activity Of Ns Extractsmentioning

confidence: 99%

“…e backs of the mice were shaved with animal clippers at 7 weeks of age. Afterward, 200 μl DMSO, 200 μl of NS extract (1,000 ppm), or 3% minoxidil was administered daily on the dorsal skin for 3 weeks using a brush, as described in previous studies [23,24,26]. At 21 days, the mice were anesthetized via intraperitoneal injection of 1.2% avertin to obtain dorsal skin samples.…”

Section: Experimental Animalsmentioning

confidence: 99%

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Hair Growth Promotion Effect of Nelumbinis Semen Extract with High Antioxidant Activity

Park

Jin

Jung

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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This study investigated the hair regeneration promotion and hair loss prevention properties of Nelumbinis Semen (NS) extract in vitro and in vivo. The effect of NS on the proliferation and migration of human dermal papilla cells (hDPCs) was measured in vitro via CCK-8 and scratch migration assays, after which the antioxidant activity of NS was also quantified. NS extracts were then applied to the back of 7-week-old C57BL/6 mice for 3 weeks to monitor hair growth patterns and hair follicle (HF) histology. The mice were divided into three groups: negative control group (NC; DMSO), positive control group (PC; 3% minoxidil), and experimental group (NS extract 1,000 ppm). Moreover, to study the molecular mechanisms by which NS extract regenerates hair growth, real-time PCR was used to analyze factors related to the hair growth cycle. The NS extracts were found to possess high antioxidant properties due to their high flavonoid contents and electron-donating ability. Moreover, NS extracts enhanced hDPC proliferation and migration in a concentration-dependent manner (15.63–125 ppm). The hair growth index and growth area of the NS group (2.81 score, 81%) on day 14 were higher than those of the PC group (2.65 score, 68%) ( p < 0.05 ). Additionally, the HFs of the NS group were located deep in the subcutis, similar to the PC group with developed hair roots. Moreover, the mRNA expression of VEGF and IGF-1 was higher in the NS group compared to the PC group, whereas TGF-β1 expression was lower ( p < 0.05 ). Our findings indicate that NS modulates hair growth by increasing IGF-1 and VEGF expression while inhibiting that of TGF-β1. Therefore, our findings suggest that NS extract is a promising new hair loss treatment derived from a natural substance that helps promote hair growth and prevent hair loss.

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Section: Discussionmentioning

confidence: 99%

Section: Antioxidant Activity Of Ns Extractsmentioning

confidence: 99%

Section: Experimental Animalsmentioning

confidence: 99%

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Hair Growth Promotion Effect of Nelumbinis Semen Extract with High Antioxidant Activity

Park

Jin

Jung

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…For example, Geranium sibiricum L. (Geraniaceae) extract (Boisvert et al 2017) and Miscanthus sinensis var. purpurascens Andersson (Gramineae) flower extract (Jeong et al 2018) have exhibited propensity to not only induce the anagen phase but to maintain it. In addition, Zhang et al (2013) observed that topical application of Thuja orientalis L. (Cupressaceae) extract in C57BL6/N mice induced an earlier anagen phase and prolonged the mature anagen phase, whereas the extract of Polygonum multiflorum Thunb.…”

Section: Discussionmentioning

confidence: 99%

Hair growth potential of Salvia plebeia extract and its associated mechanisms

Jin

Zhang

Yap

et al. 2020

Pharmaceutical Biology

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Context: Although Salvia plebeia (SP) R. Brown (Labiatae) is known to possess various biological activities, the effects of SP on hair growth have not been elucidated. Objective: To investigate the hair growth potential of SP extract by using human dermal papilla cells (hDPCs) and C57BL/6 mice. Materials and methods: The entire SP plant sample was ground into powder and extracted with 99.9% methyl alcohol. Various concentrations of SP extract were added to hDPCs to evaluate the proliferation, migration, and factors related to hair growth and cycling. Effect of topical SP administration on hair regrowth was tested in vivo in male C57BL/6 mice for 21 days. Results: SP extract significantly increased the proliferation of cultured hDPCs at doses of 15.6 and 31.3 lg/mL compared to control group by 123% and 132%, respectively. Expression of hepatocyte growth factor increased while the level of TGF-b1 and SMAD2/3 decreased when treated with SP extract. At the molecular level, the extract activated Wnt/b-catenin signalling by raising b-catenin and phospho-GSK3b expression. SP extract also exerted anti-apoptotic and proliferative effects in hDPCs by increasing the Bcl-2/Bax ratio and activating cell proliferation-related proteins, ERK and Akt. Finally, the extract caused an induction of the anagen phase leading to significantly enhanced hair growth in treated male mice. Discussion and conclusion: Our results indicate that SP extract has the capacity to activate hDPCs into a proliferative state to promote hair growth. Further research is necessary to determine the bioactive components and their mechanisms of action responsible for SP-related hair growth effect.

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“…The research on herbs and active constituents as hair growth promoters has been driven by their less toxicity, easy availability, low‐cost, patient compliance and more than one mode of biochemical action . Specific effects of herbal extracts, polyherbal formulations, flavonoids and natural compounds on DPCs have been examined . In vivo hair growth promoting effects as proof of concept are also mentioned.…”

Section: Hair Growth Promoting Effectmentioning

confidence: 99%

Review of Hair Follicle Dermal Papilla cells as in vitro screening model for hair growth

Madaan

Verma

Singh

et al. 2018

Intern J of Cosmetic Sci

102

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Hair disorders such as hair loss (alopecia) and androgen dependent, excessive hair growth (hirsutism, hypertrichosis) may impact the social and psychological well-being of an individual. Recent advances in understanding the biology of hair have accelerated the research and development of novel therapeutic and cosmetic hair growth agents. Preclinical models aid in dermocosmetic efficacy testing and claim substantiation of hair growth modulators. The in vitro models to investigate hair growth utilize the hair follicle Dermal Papilla cells (DPCs), specialized mesenchymal cells located at the base of hair follicle that play essential roles in hair follicular morphogenesis and postnatal hair growth cycles. In this review, we have compiled and discussed the extensively reported literature citing DPCs as in vitro model to study hair growth promoting and inhibitory effects. A variety of agents such as herbal and natural extracts, growth factors and cytokines, platelet-rich plasma, placental extract, stem cells and conditioned medium, peptides, hormones, lipid-nanocarrier, light, electrical and electromagnetic field stimulation, androgens and their analogs, stress-serum and chemotherapeutic agents etc. have been examined for their hair growth modulating effects in DPCs. Effects on DPCs' activity were determined from untreated (basal) or stress induced levels. Cell proliferation, apoptosis and secretion of growth factors were included as primary end-point markers. Effects on a wide range of biomolecules and mechanistic pathways that play key role in the biology of hair growth were also investigated. This consolidated and comprehensive review summarizes the up-to-date information and understanding regarding DPCs based screening models for hair growth and may be helpful for researchers to select the appropriate assay system and biomarkers. This review highlights the pivotal role of DPCs in the forefront of hair research as screening platforms by providing insights into mechanistic action at cellular level, which may further direct the development of novel hair growth modulators.

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Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice

Abstract: The MSP flower extract may have hair growth-promotion activities.

Cited by 11 publications

References 20 publications

Hair Growth Promotion Effect of Nelumbinis Semen Extract with High Antioxidant Activity

Hair Growth Promotion Effect of Nelumbinis Semen Extract with High Antioxidant Activity

Hair growth potential of Salvia plebeia extract and its associated mechanisms

Review of Hair Follicle Dermal Papilla cells as in vitro screening model for hair growth

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