Effect of microgrooved poly‐l‐lactic (PLA) surfaces on proliferation, cytoskeletal organization, and mineralized matrix formation of rat bone marrow cells

Abstract

Previous experiments showed that microgrooved substrate surfaces can influence the in vitro behavior of osteoblast-like rat bone marrow (RBM) cells. Cellular morphology and matrix deposition can be directed by substrate chemistry and topography. RBM cells cultured on poly-l-lactic acid (PLA) exhibit increased mineralization and alkaline phosphatase activity compared to polystyrene substrates. Consequently, the purpose of the present in vitro study is to further evaluate the behavior of RBM cells on microgrooved (groove depth 0.5 to 1.5 microns, groove and ridge width 1 to 10 microns) polystyrene (PS) and PLA surfaces. Besides grooved, also smooth control surfaces were made. Our results confirmed that microtextured surfaces are capable of influencing the behavior of the osteoblast-like RBM cells in vitro. Microtopography did not influence the RBM proliferation rate, or cellular actin organization. However, confocal laser scanning microscopy showed that cellular attachment is dependent on the applied material. Also clear differences were found between textured PS and PLA, with regard to the calcium content. We therefore conclude, that the application of microtextures could possibly influence the bone regeneration around biodegradable PLA devices.

Behavior of osteoblast-like cells on fibronectin or BMP-2 immobilized surface

“…The biological evaluation for BMP-2 immobilized surface was carried out by the mesurement of ALP activity and expression of osteocalcin mRNA in this study. These methods are commonly used at this moment (7,12,14). So this study indicated that a fi bronectin immobilized surface increased the number of adhering cells in the early stages, and that a BMP-2 immobilized surface stimulates an osteogenetic function.…”

“…Generally, the healing of a wound around an implant is infl uenced by material-related factors, e.g., surface topography, mechanical properties, and physicochemical properties of the implant material. Many reports have dealt with the surface modifi cation and the control of cell behavior on surface topography (11,(13)(14)(15)(16)27). Surface topography can influence cell orientation based on the concept of contact guidance (20) and expressions of cell functions (13)(14)(15)(16)27).…”

Epithelial contact guidance on well-defined micro- and nanostructured substrates

“…Various cell types display contact guidance when cultured on groove and ridge patterns with lateral dimensions in the micrometer range (Clark et al, 1990;den Braber et al, 1995;den Braber et al, 1996a;den Braber et al, 1996b;den Braber et al, 1998;Flemming et al, 1999;Matsuzaka et al, 2000;van Kooten et al, 1998;Walboomers et al, 1999a;Walboomers et al, 1999b;Wojciak-Stothard et al, 1995b;Wojciak-Stothard et al, 1996). Profound differences in cytoskeletal organization have been found between cells elongated and aligned along these topographic features and cells cultured on smooth surfaces.…”

Bio-Functionalization of Titanium Surfaces for Dental Implants

“…1,2) Surface roughness alters osteoblast proliferation, differentiation, and matrix production in vitro, and plays a role in determining the phenotypic expression of cells in vivo. 3,4) Surface chemistry involves the adsorption of proteins, bacteria, and cells on biomaterials. This adsorption reflects the * Corresponding author: yosinari@tdc.ac.jp affinity between two substances, and the strength of adsorption follows the order: chemical adsorption including covalent bonds and ionic bonds > electrostatic force found in electrokinetic potential or zeta potential > hydrogen bonds involved in hydrophilic groups such as -OH, -COOH, and -NH 2 > hydrophobic interaction (i.e., adsorption of hydrophobic substances in water) > van der Waals forces.…”