Behavior of osteoblast-like cells on fibronectin or BMP-2 immobilized surface

Abstract

Property modifi cation of an implant surface is known to infl uence the behavior of cells surrounding implant material. This study examined the osteogenic cell behavior on protein immobilized surface in vitro. Following plasma surface modifi cation, fi bronectin or bone morphogenetic protein-2 (BMP-2) was immobilized. The number of cells adhering to fi bronectin-immobilized surface increased after 1 and 2 h of incubation compared with non-immobilized surface. Alkaline phosphatase activity and osteocalcin mRNA expression of the osteogenic cells on the BMP-2 immobilized surface was greater than that on the non-immobilized surface. This study demonstrated that protein can be immobilized to a polystyrene surface after treatment with O 2 plasma and that the osteogenic cells surrounding a biomaterial can be controlled by the immobilization of protein to the biomaterial.

Effective Immobilization of BMP-2 Mediated by Polydopamine Coating on Biodegradable Nanofibers for Enhanced in Vivo Bone Formation

“…When 500 μL of BMP-2 at 250 ng/mL or 500 ng/mL was used to treat 1 cm 2 of polydopamine-coated nanofibers, the DP-B500 group showed more immobilized BMP-2 (124 ± 9 ng/cm 2 ) than the DP-250 group (61 ± 8 ng/cm 2 ). These results matched findings on the amount of BMP-2 immobilized on to O 2 plasma-treated polystyrene surfaces (44.2 ng/cm 2 ) and gold surfaces modified by self-assembled monolayer formation (70–80 ng/cm 2 ). , In this study, BMP-2 immobilization efficiency was approximately 80% in both groups. This high efficiency was comparable to previous reports on immobilization of RGD peptide (80%) and glial cell line-derived neurotrophic factor (90%) on polydopamine-coated polystyrene substrates, and immobilization of BMP-2 on chitosan-grafted titanium surfaces (81%). , …”

Controlled spatial and conformational display of immobilised bone morphogenetic protein-2 and osteopontin signalling motifs regulates osteoblast adhesion and differentiation in vitro

“…This is in agreement with the work of Saito et al (2003) who showed that a synthetic BMP2 knuckle peptide was able to induce alkaline phosphatase activity in C2C12 cells but less effectively than srBMP2 [16]. Previous work [32,33] has shown that immobilised BMP2 more effectively activates differentiation of osteoblasts than soluble BMP2. Tol-BMP-ST was immobilised on gold surfaces, back-filled with TEG-thiol and then compared with soluble recombinant BMP-2 for the ability to regulate osteoblast activity and bone formation.…”

Behavior of rat periodontal ligament cells on fibroblast growth factor‐2‐immobilized titanium surfaces treated by plasma modification

“…These surfaces were hydrophilic, and O 2 ‐functional groups were able to support adsorption of various types of peptide and protein 14. Matsuzaka et al13 reported that immobilization of protein on a biomaterial enables control of cell behavior, for example, the number of osteoblast‐like cells initially attached on fibronectin immobilized on O 2 ‐plasma‐treated surface. Yoshinari et al14 reported that protein adsorption on more hydrophobic interaction, and the adsorption characteristics of proteins, depends on the residual groups of amino acid in proteins.…”

“…growth factors) on the surface. Matsuzaka et al 13 reported that bone morphogenetic protein-2 (BMP-2) and fibronectin could be immobilized using oxygen (O 2 ) plasma treatment. Yoshinari et al 14,15 reported that fibronectin and simvastatin were immobilized using plasma treatment.…”