Behavior of rat periodontal ligament cells on fibroblast growth factor‐2‐immobilized titanium surfaces treated by plasma modification

Abstract

The aim of this study was to investigate the behavior of rat periodontal ligament (PDL) cells cultured on fibroblast growth factor-2 (FGF-2)-immobilized titanium surfaces treated by oxygen (O2) plasma. We used cell disks (15 mm in diameter), and 35-mm culture dishes sputter-coated with titanium. These were treated with oxygen plasma and dipped in FGF-2 solution. Immobilized FGF-2 was visualized with a confocal laser-scanning microscope, and its weight was calculated to be approximately 22.6 ng/cm(2) using a quartz crystal microbalance-dissipation apparatus. The PDL cells were obtained from rat incisors. Cells from fourth subculture were seeded onto the FGF-2-immobilized titanium surface. Proliferation ratio, alkaline phosphatase (ALP) activity, and expressions of type I collagen and vascular endothelial growth factor (VEGF) mRNAs were evaluated. Proliferation ratio and expressions of type I collagen and VEGF mRNAs were significantly higher, whereas ALP activity was significantly lower in FGF-2-immobilized cells than in control group (p < 0.05). These findings suggest that oxygen plasma modification can immobilize FGF-2 onto a titanium surface. Immobilized FGF-2, although inferior to culture medium with FGF-2, influenced the proliferation of PDL cells and might have promoted collagen and vascular synthesis.

Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

“…35 Application of FGF2 to periodontal tissue regeneration has been previously reported. 36,37 FGF2 can be immobilized on to Ti surface 24 and hydroxyapatite-chitosan scaffolds. 38 The immobilization procedure used in this study as well as the chemical inertness and stability of TiO 2 ensure that there is very little possibility for the FGF2 to react with the TiO 2 NT array.…”

“…24 After FGF2 immobilization, the samples were gently washed thrice with phosphate buffered saline (PBS, pH = 7.4) for 5 minutes, treated with rabbit anti-bFGF polyclonal antibody (1:200; Sigma-Aldrich) as the primary antibody at 4°C overnight, and washed thrice with PBS. The samples were then treated with fluorescein isothiocyanate-labeled goat antirabbit immunoglobulin G (1:200; Bois, People's Republic of China) for 1 hour at 37°C and washed thrice in PBS (pH = 7.4).…”

“…23 FGF2 can be immobilized on Ti surfaces pretreated with oxygen plasma, 24 and the TiO 2 nanotubular surface benefits drug control release 25 and immobilization of other growth factors such as, bone morphogenetic protein 2 as well.…”

Immobilization of Growth Factors for Cell Therapy Manufacturing

“…Plasma treatment involves the introduction of functional groups to surfaces, which can be utilized in various chemistries, such as carbodiimide coupling, to immobilize GFs (Zhang et al, 2012). This particular technique has been used for the immobilization of bone morphogenetic protein-4 (BMP-4), BMP-2, bFGF, and FGF-2 (Puleo et al, 2002;Shen et al, 2008Shen et al, , 2009Kokubu et al, 2009). Silane and imine coupling are other useful techniques for GF immobilization, in which the formed covalent bonds can be hydrolyzed under physiological conditions, allowing control on subsequent release of the GF (Cabanas-Danés et al, 2018).…”

Surface Modification of Dental Implant Improves Implant–Tissue Interface

“…Matsuzaka et al reported that bone morphogenetic protein-2(BMP-2) and fibronectin could be immobilized using oxygen plasma treatment. Immobilization of GFG-2 on an implant using modified surface topography might allow proliferation of periodontal ligament cells around the implant [25,26]. 3.7 Calcium Phosphate (Ca-P) Coating by Plasma Spraying…”