Epithelial contact guidance on well-defined micro- and nanostructured substrates

Teixeira, Ana I.; Abrams, George A.; Bertics, Paul J.; Murphy, Christopher J.; Nealey, Paul F.

doi:10.1242/jcs.00383

Cited by 899 publications

(932 citation statements)

References 51 publications

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“…Increasing feature height, while keeping constant inter-feature length, causes the space between adjacent grooves to become impervious to the cell membrane and integrins and therefore a decrease in adhesion stability occurs [70,71]. Analogous results have been observed on substrates presenting arrays of nanometric pits.…”

Section: Topo-cue-mediated Crosstalkmentioning

confidence: 60%

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Ventre

Causa

Netti

2012

J. R. Soc. Interface.

158

153

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The development of novel biomaterials able to control cell activities and direct their fate is warranted for engineering functional biological tissues, advanced cell culture systems, single-cell diagnosis as well as for cell sorting and differentiation. It is well established that crosstalk at the cell–material interface occurs and this has a profound influence on cell behaviour. However, the complete deciphering of the cell–material communication code is still far away. A variety of material surface properties have been reported to affect the strength and the nature of the cell–material interactions, including biological cues, topography and mechanical properties. Novel experimental evidence bears out the hypothesis that these three different signals participate in the same material–cytoskeleton crosstalk pathway via adhesion plaque formation dynamics. In this review, we present the relevant findings on material-induced cell response along with the description of cell behaviour when exposed to arrays of signals—biochemical, topographical and mechanical. Finally, with the aid of literature data, we attempt to draw unifying elements of the material–cytoskeleton–cell fate chain.

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Section: Topo-cue-mediated Crosstalkmentioning

confidence: 60%

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Ventre

Causa

Netti

2012

J. R. Soc. Interface.

158

153

View full text Add to dashboard Cite

show abstract

“…Also, the morphology of MCF10A cells on the 400 nm line pattern showed contact guidance along the line direction presumably with more clusters of focal adhesions at the leading edges, corresponding to earlier results. 22 By contrast, MCF7 cells showed starshaped morphology (typical characteristics of cancer cell) on the 400 nm pillar and line patterns. It is noted that the elongation of MCF7 cells was not pronounced on the line pattern compared to MCF10A cells, suggesting that the adhesion of cancer cells would be relatively weak.…”

Section: Adhesions Of Mcf10a and Mcf7 Cellsmentioning

confidence: 98%

“…[19][20][21] Recently, extensive efforts have been made to understand/control cell adhesions on various surface nanostructures using conventional or unconventional lithographic techniques. [22][23][24][25] A microfluidic system is advantageous for analysis of cell adhesion in various aspects because of small sample consumption, use of multiple parameters, and generation of large shear stress. 26,27 By integrating nanostructured surfaces with a microfluidic system, we herein devise a simple, label-free cell separation and enrichment scheme based on standard soft lithography and microfluidics protocol.…”

Section: Introductionmentioning

confidence: 99%

Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

et al. 2007

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A label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the adhesion difference between normal epithelial and cancer cells, flat or nanostructured polymer surfaces (400 nm pillars, 400 nm perpendicular, or 400 nm parallel lines) were constructed on the bottom of polydimethylsiloxane (PDMS) microfluidic channels in a parallel fashion using a UV-assisted capillary moulding technique. The adhesion of human breast epithelial cells (MCF10A) and cancer cells (MCF7) on each channel was independently measured based on detachment assays where the adherent cells were counted with increasing flow rate after a pre-culture for a period of time (e.g., one, two, and four hours). It was found that MCF10A cells showed higher adhesion than MCF7 cells regardless of culture time and surface nanotopography at all flow rates, resulting in label-free separation and enrichment of cancer cells. For the cell types used in our study, an optimum separation was found for 2 hours pre-culture on the 400 nm perpendicular line pattern followed by flow-induced detachment at a flow rate of 200 ml min 21 . The fraction of MCF7 cells was increased from 0.36 ¡ 0.04 to 0.83 ¡ 0.04 under these optimized conditions.

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“…However, no significant synergistic effect was observed for gravity//microgrooves. Recent reports have shown that contact guidance is closely related to three-dimensional pattern size [11,12]. Therefore, the pattern size should be optimized to weaken the unfavorable effect of gravitational field by inducing more pronounced contact guidance.…”

Section: Contact Guidance Testingmentioning

confidence: 99%

Effects of contact guidance and gravity on L929 cell orientation

et al. 2011

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Contact guidance and external force field are two important factors that influence cell orientation. Microgroove-like patterns on PDMS substrates were fabricated using soft-lithography, and the effects of gravitational field direction relative to the pattern, on L929 cell orientation were investigated. The majority of cells were aligned along the grooves when gravity was parallel to the groove direction, because of contact guidance. When gravity was perpendicular to the groove direction, cell alignment decreased noticeably. Although contact guidance induced by the pattern played a dominant role in determining cell orientation, gravity also had an important influence when the field direction was perpendicular to the groove direction. No synergistic influence on cell alignment was observed when the field direction was parallel to the groove direction.

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Epithelial contact guidance on well-defined micro- and nanostructured substrates

Abstract: the native basement membrane can profoundly affect epithelial cell behavior.

Cited by 899 publications

References 51 publications

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

Effects of contact guidance and gravity on L929 cell orientation

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