Effect of microcystin-LR and cyanobacterial extract from polish reservoir of drinking water on cell cycle progression, mitotic spindle, and apoptosis in CHO-K1 cells

A review of reproductive toxicity of microcystins

“…Lankoff et al [76] demonstrated that both pure MC-LR and cyanobacterial bloom extract effectively deregulated the cell cycle of CHO-K1 cells by damaging the mitotic spindle apparatus and induced both apoptosis and necrosis. Similarly, Gácsi et al [77] also reported that MC-LR reduced the number of mitotic cells, pre- [82] vented chromosome formation and induced apoptosis and necrosis in CHO-K1 cells, associated with shortening and degradation of major cytoskeletal components such as microfilaments (MFs) and microtubules (MTs), resulting in reproductive toxicity.…”

Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels

“…The toxic effects of MCLR on the cytoskeletal structures have been extensively investigated in zebrafish, rat and different cell types [12,22,[50][51][52][53][54]. All these studies detected altered expression of cytoskeleton and its associated proteins.…”

The Biochemical Alteration and Dna Damage in Rats (Rattus Rattus) After Chronic Intraperitoneally Injection to Purified Microcystin-Lr From Anabaena Circinalis

“…The DNA damage that induces by MC-LR can be related with apoptosis rather than with genotoxicity [41] while Lankoff [42] also demonstrate MC-LR induce DNA damage and genotoxicity through its ability to cause genetic instability in erythrocytes in bone marrow of mice injected 45 µg MCLR/kg bw for 24 hrs and MC-LR stimulate a 2-fold increase The result of presents study had been showed, the parameters of comet assay were significantly differences between treatment and control group, the highest values of comet length µm, tail length µm, and tail moment µm in liver of rats were recorded at dose 9 µg/kg b.w. as compared with control group, the statistical analysis appeared positive significant correlation between DNA damage markers and the dose of MC-LR, biochemical markers.…”

“…The comet length was positively correlated with the level of DNA breakage in cell because the distribution the comet was heterogeneous [19]. The highest value of the markers of DNA damage would be increased with increasing the toxicant dose this may be due to insufficient produce of antioxidant defense systems to scavenge the ROS that is generated by MC-LR which lead to finding their way across nuclear membrane indicating DNA strand breakage [42][43][44][45][46].…”