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In this study, biocontrol of harmful effect of cyanobacterial blooms and their toxins by “flocculation-biosorption” was achieved. Five fungal species were isolated from decayed cyanobacterial bloom which are: Aspergillus fumigatus, A. niger, Penicillium, Trichoderma ressei and Mucor rouxii. We chose the last species’ pellets because they are the most stable and cocultured with Anabeana sp. (1:5 fungal: cyanobacteria ratio) of dry weight, Harvest Efficacy HE% by fungal pellets started after 12h of co-culturing about (4%) and almost complete harvesting after 48h with (98%), then we add 0.1g of Magnetite nano Fe3o4 to facilitate removing cyanobacterial blooms. Microcystin-LR extracted from Anabaena sp. were purified and collected by preparative high-performance liquid chromatography (HPLC) was 75.1 (µg ml-1), M. rouxii pellet absorbed about 85% of Microcystin-LR after 72 h of incubation at 25 °C.

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The presents study was designed to evaluate the DNA damage markers, antioxidants makers and lipid peroxidation in breast cancer patients. About 5ml of venous blood was collected from 10 healthy persons and 30 breast cancer patients before chemotherapy. Comet assay used to measure DNA damage and antioxidants were assessed biochemically.Lipid peroxidation was estimated by the Thiobarbituric acid assay.The results of present study showed that the DNA damage markers such as comet length, tail length, DNA percentage in tail and tail moments were significantly increased at p<0.05 in Breast cancer patients as compared with healthy control group. Also biochemical markers such as SOD and CATinsignificantly high in patients and MDA was significantly elevated in the patients while GPx and GSH decreased as compared with the healthy control.This indicates high level of ROS, tumor transformation and invasion that play as a good early marker for cancer recurrence. The COMET assays described in this study help in evaluating single and double-strand breaks and monitor the ability of the cells to repair such damage.

Silver nanoparticles (AgNPs) were produced utilizing Marasmius palmivorus MG717877.1 in the current investigation. The fungal cell filtrate was used to accomplish external production of silver nanoparticles (AgNPs) from silver nitrate (AgNo3) solution. The aqueous silver (Ag) ions in a 1mM AgNo3 aqueous solution were decreased when it was exposed to fungal cell filtrate, resulting in highly stable AgNPs. When challenged with 1mM AgNo3 solution, the fungal biomass acquired AgNPs on its surface, inside the cytoplasmic membrane, and within the cytoplasm in 72 hours. UV was used to characterize the AgNPs that had been produced. For M.palmivorus MG717877.1, the greatest absorbance of AgNPs was recorded at 400nm in the visible spectrum. Furthermore, the FTIR and SEM analyses of silver nanoparticles on these fungus were used to characterize silver nanoparticles. In addition, we tested the antifungal activity of M.palmivorus MG717877.1 at various concentrations, including 25, 50, 100, and 150 mg/ml. The findings revealed that silver nanoparticles produced in a concentration-dependent manner have strong antifungal activity on isolates. At 150 mg of AgNPs, the highest decrease was seen for this isolate.