Effect of Mesenchymal Stem Cell Application on the Distracted Bone Microstructure: An Experimental Study

Hadidi, Yasser Nabil El; Kassaby, Marwa El; Ahmed, Salah Abd El Fatah; Khamis, Nahed Samy

doi:10.1016/j.joms.2016.03.021

Cited by 14 publications

(21 citation statements)

References 39 publications

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“…The stained samples were evaluated histomorphometrically. 6 After decalcification, bony samples were sectioned longitudinally. Each histological section was divided into three zones: proximal, middle, and distal zones.…”

Section: Assessmentmentioning

confidence: 99%

The Effect of Bone Marrow Mesenchymal Stem Cells Application on Distracted Bone Quality during Rapid Rate of Distraction Osteogenesis

Kassaby

Kader

Khamis

et al. 2018

Craniomaxillofacial Trauma & Reconstruction

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Distraction osteogenesis (DO) bone regenerate usually suffers from an inferior quality especially with rapid rate. This study was conducted to investigate the effect of mesenchymal stem cells (MSCs) application on different rates of distraction bone quality. Twenty-four goats were divided into group A with standard DO and group B with rapid distraction osteogenesis (RDO) both aided by MSCs. Group C with standard DO and group (D) with RDO were controls. Kruskal-Wallis test and Conover's post hoc analysis was used to evaluate significance ( = 0.05). Histomorphometry showed a strongly significant (SS) increase ( = 0.00036) in trabecular bone (TB) in group A (TB = 174.7 µm, SD = 33.5) and group B (TB = 166.8 µm, SD = 14) compared with group C (TB = 115.4 µm, SD = 19.6) and group D (TB = 86.1 µm, SD = 9.3). There was SS decrease ( = 0.00093) in osteoid percentage (OP) in group A (OP = 13.4%, SD = 2) and group B (OP = 11.5%, SD = 6.5) compared with group C (OP = 27.3, SD = 3.5) and group D (OP = 26.2%, SD = 2.6). Energy dispersive X-ray showed a nonsignificant increase ( = 0.11) in calcification (Ca %) in group A (Ca % = 17.6%, SD = 4.9) and group B (Ca % = 17.6%, SD = 4.3) compared with group C (Ca % = 14.2%, SD = 6.7) and group D (Ca % = 11.5%, SD = 2.4). MSCs application improved microscopic bone quality during standard DO and RDO. However, macroscopic bone quality improvement still needs further investigation.

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Section: Assessmentmentioning

confidence: 99%

The Effect of Bone Marrow Mesenchymal Stem Cells Application on Distracted Bone Quality during Rapid Rate of Distraction Osteogenesis

Kassaby

Kader

Khamis

et al. 2018

Craniomaxillofacial Trauma & Reconstruction

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“…This may result in fibrous union or nonunion. 10,29,34 In order to enhance the quality and quantity, and the time to bone formation in DO, DO animal models were developed to test bone regeneration by MSC therapy approaches. 35 …”

Section: Discussionmentioning

confidence: 99%

“…All animals used were skeletally mature, except the goats used by El Hadidi et al 29 which were of an age comparable with childhood. Older rats display delayed healing of femoral fractures.…”

Section: Discussionmentioning

confidence: 99%

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Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

Yang

Lin

Wang

et al. 2017

Bone & Joint Research

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ObjectivesDistraction osteogenesis (DO) mobilises bone regenerative potential and avoids the complications of other treatments such as bone graft. The major disadvantage of DO is the length of time required for bone consolidation. Mesenchymal stem cells (MSCs) have been used to promote bone formation with some good results.MethodsWe hereby review the published literature on the use of MSCs in promoting bone consolidation during DO.ResultsStudies differed in animal type (mice, rabbit, dog, sheep), bone type (femur, tibia, skull), DO protocols and cell transplantation methods.ConclusionThe majority of studies reported that the transplantation of MSCs enhanced bone consolidation or formation in DO. Many questions relating to animal model, DO protocol and cell transplantation regime remain to be further investigated. Clinical trials are needed to test and confirm these findings from animal studies.Cite this article: Y. Yang, S. Lin, B. Wang, W. Gu, G. Li. Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis: A contemporary review of experimental studies. Bone Joint Res 2017;6:385–390. DOI: 10.1302/2046-3758.66.BJR-2017-0023.

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“…A body of pre-clinical research over the past 20 years indicates that addition of cells enhances the amount and quality of new bone generated by DO. [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] These studies have used stem cells or osteogenically differentiated cells with success. This has been shown in both mandibular [12,13,[16][17][18][19]25,26] and long bone distraction [11,14,15,[20][21][22][23][24] and could theoretically decrease the consolidation time.…”

Section: Introductionmentioning

confidence: 99%

Systemically administered MSCs given 24hrs after osteotomy do not affect bone formation in rat distraction osteogenesis

Guevara

Toth

Kim

et al. 2018

Preprint

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Distraction osteogenesis is a unique postnatal bone formation employed by orthopaedic surgeons to treat many conditions, however, the overall time to external frame removal can be extensive. Any strategies that accelerate healing would improve patient care. Distraction osteogenesis research in the past decade has shown that direct stem cell implantation enhances new bone formation. Systemic implantation would be more clinically desirable. Systemically delivered stem cells have been shown to home to a mandibular distraction site; however, effects on bone formation have not been studied. Ten-week-old, male Sprague-Dawley rats underwent surgery to implant an external fixator-distractor and an osteotomy was performed. Twenty-four hours postoperatively, each rat received tail vein injections of either saline or 10^6 fluorescently labeled primary mesenchymal stem cells. Animals in the validation groups were euthanized two days after surgery and the femora processed for histology. Animals in the experimental groups were given five days of latency, then the femur was lengthened once daily for five days (0.75mm/day, 3.75mm total). Following four weeks of consolidation, the animals were euthanized and the femora were evaluated by microCT and histology to quantify new bone formation. Labeled stem cells were found at the distraction site in validation animals. However, there were no differences in any bone or soft tissue outcomes. Systemic stem cell administration 24 hours after surgery does not improve DO outcomes. It is possible that the animal model was not challenging enough to discriminate any augmentation provided by stem cells.

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Effect of Mesenchymal Stem Cell Application on the Distracted Bone Microstructure: An Experimental Study

Cited by 14 publications

References 39 publications

The Effect of Bone Marrow Mesenchymal Stem Cells Application on Distracted Bone Quality during Rapid Rate of Distraction Osteogenesis

The Effect of Bone Marrow Mesenchymal Stem Cells Application on Distracted Bone Quality during Rapid Rate of Distraction Osteogenesis

Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

Systemically administered MSCs given 24hrs after osteotomy do not affect bone formation in rat distraction osteogenesis

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