Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

Yang, Yanhua; Lin, Sien; Wang, Bin; Gu, Weidong; Li, G.

doi:10.1302/2046-3758.66.bjr-2017-0023

Cited by 25 publications

(20 citation statements)

References 51 publications

(65 reference statements)

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“…Therefore, the scientists are constantly exploring new medical tools [14,20,21,22,23]. The emergence of stem cell therapy brings new hope for the treatment of these diseases [24,25]. The homing and low immunogenicity of MSC make them ideal seed cells for stem cell therapy [26].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Biological Characteristics and Transcriptome Profiles of Mesenchymal Stem Cells from Different Canine Tissues

Zhan

El‐Ashram

Luo

et al. 2019

IJMS

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Mesenchymal stem cells (MSCs) are the most promising seed cells for cell therapy. Comparing the biological and transcriptome gene characteristics of MSCs from different sources provides an important basis for the screening of clinically used cells. The main purpose of this experiment was to establish methods for the isolation and culture of MSCs from five different canine sources, including adipose tissue, bone marrow, umbilical cord, amniotic membrane, and placenta, and compare biological and transcriptome characteristics of MSCs, in order to provide a basis for the clinical application of canine MSCs. MSCs were isolated from Chinese pastoral dogs, and the following experiments were performed: (1) the third, sixth, and ninth generations of cells were counted, respectively, and a growth curve was plotted to calculate the MSC population doubling time; (2) the expression of CD34 and CD44 surface markers was studied by immunofluorescence; (3) the third generation of cells were used for osteogenetic and adipogenic differentiation experiments; and (4) MSC transcriptome profiles were performed using RNA sequencing. All of the five types of MSCs showed fibroblast-like adherent growth. The cell surface expressed CD44 instead of CD34; the third-generation MSCs had the highest proliferative activity. The average population doubling time of adipose mesenchymal stem cells (AD-MSCs), placenta mesenchymal stem cells (P-MSCs), bone marrow mesenchymal stem cells (BM-MSCs), umbilical cord mesenchymal stem cells (UC-MSCs), and amniotic mesenchymal stem cells (AM-MSCs) were 15.8 h, 21.2 h, 26.2 h, 35 h, and 41.9 h, respectively. All five types of MSCs could be induced to differentiate into adipocytes and osteoblasts in vitro, with lipid droplets appearing after 8 days and bone formation occurring 5 days after AD-MSC induction. However, the multilineage differentiation for the remaining of MSCs was longer compared to that of the AD-MSCs. The MSC transcriptome profiles showed that AD-MSC and BM-MSCs had the highest homology, while P-MSCs were significantly different compared to the other four types of MSCs. All the isolated MSCs had the main biological characteristics of MSCs. AD-MSCs had the shortest time for proliferation, adipogenesis, and osteogenic differentiation.

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Section: Introductionmentioning

confidence: 99%

A Comparative Study of Biological Characteristics and Transcriptome Profiles of Mesenchymal Stem Cells from Different Canine Tissues

Zhan

El‐Ashram

Luo

et al. 2019

IJMS

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“…The long treatment duration of DO leads to frequent complications; therefore, shortening the treatment period may provide significant clinical benefits. Transplantation of MSCs could accelerate new bone formation, which represents a potential strategy for shortening DO treatment time [14]. Although autologous MSC transplantation has advantages with regard to safety and efficiency in tissue regeneration, the procedure has several inevitable disadvantages, including source limits, risk of donor site infection and age-related impairment of MSC function [21].…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that MSCs are effective in promoting bone healing and enhancing bone consolidation in DO animal models [1,7,10À13]. The authors' recent review article addressed the successful applications of MSCs in DO systematically in various animal species (mice, rabbits, dogs, sheep), bone sites (femur, tibia, skull), DO protocols and cell transplantation methods [14]. Systemic reviews from other groups have also addressed the efficacy of locally applied MSCs in promoting bone formation in mandible DO animal models [15,16].…”

Section: Introductionmentioning

confidence: 99%

Local administration of allogeneic or autologous bone marrow-derived mesenchymal stromal cells enhances bone formation similarly in distraction osteogenesis

Pan

et al. 2021

Cytotherapy

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Background aims: Distraction osteogenesis (DO) is a surgical technique to promote bone regeneration that requires a long time for bone healing. Bone marrow-derived mesenchymal stromal cells (MSCs) have been applied to accelerate bone formation in DO. Allogeneic MSCs are attractive, as they could be ready to use in clinics. Whether allogeneic MSCs would have an effect similar to autologous MSCs with regard to promoting bone formation in DO is still unknown. This study compares the effect of autologous MSCs versus allogeneic MSCs on bone formation in a rat DO model. Methods: Rat bone marrow-derived MSCs were isolated, characterized and expanded in vitro. Adult rats were subjected to right tibia transverse osteotomy. On the third day of distraction, each rat received one injection of phosphate-buffered saline (PBS), autologous MSCs or allogeneic MSCs at the distraction site. Tibiae were harvested after 28 days of consolidation for micro-computed tomography examination, mechanical test and histological analysis. Results: Results showed that treatment with both allogeneic and autologous MSCs promoted bone formation, with significantly higher bone mass, mechanical properties and mineral apposition rate as well as expression of angiogenic and bone formation markers at the regeneration sites compared with the PBS-treated group. No statistical difference in bone formation was found between the allogeneic and autologous MSC treatment groups. Conclusions: This study indicates that allogeneic and autologous MSCs have a similar effect on promoting bone consolidation in DO. MSCs from an allogeneic source could be used off-the-shelf with DO to achieve early bone healing.

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“…Managing massive bone loss following traumatic open fracture is challenging as normal healing is compromised by poor vascularity. Recent studies support the use of stem cells for musculoskeletal applications and improving the reparative process 1 - 3 .…”

Section: Introductionmentioning

confidence: 95%

Massive Tibial Bone Regeneration with Autologous Peripheral Blood Stem Cells using Ilizarov Bone Transport: A Case Report

2020

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Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

Cited by 25 publications

References 51 publications

A Comparative Study of Biological Characteristics and Transcriptome Profiles of Mesenchymal Stem Cells from Different Canine Tissues

A Comparative Study of Biological Characteristics and Transcriptome Profiles of Mesenchymal Stem Cells from Different Canine Tissues

Local administration of allogeneic or autologous bone marrow-derived mesenchymal stromal cells enhances bone formation similarly in distraction osteogenesis

Massive Tibial Bone Regeneration with Autologous Peripheral Blood Stem Cells using Ilizarov Bone Transport: A Case Report

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