Effect of maternal micronutrients (folic acid, vitamin B12) and omega 3 fatty acids on liver fatty acid desaturases and transport proteins in Wistar rats

Wadhwani, Nisha; Manglekar, Rupali Rahul; Dangat, Kamini; Kulkarni, Asmita; Joshi, Sadhana

doi:10.1016/j.plefa.2011.10.010

Cited by 33 publications

(23 citation statements)

References 55 publications

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“…The activity of D5D and D6D can undergo modifications due to the presence of fats, with the decisive factors being the quantity and quality of PUFAs contained in them. This is also confirmed by the results obtained by other authors [4][5][6][7]. Sunflower oil is characterized by a very high level of LA, which is an n-6 PUFA, and a small content of ALA, which is an n-3 PUFA.…”

Section: Discussionsupporting

confidence: 90%

“…Thus, the processes of elongation and desaturation depend on absolute amounts of those fatty acids as well as on the activity of enzymes (mainly desaturases) that play a key role on the pathway of these metabolic transformations. One of the factors that significantly affects desaturase activity is the kind and the composition of the supplied diet [2,[4][5][6][7][8][9]. Many studies showed the influence of low-fat diet, high-fat diet, and PUFA-enriched diet on the activity of Δ 6 -desaturase (D6D) [6,10].…”

Section: Introductionmentioning

confidence: 99%

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Heating of vegetable oils influences the activity of enzymes participating in arachidonic acid formation in Wistar rats

2015

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Heating of vegetable oils influences the activity of enzymes participating in arachidonic acid formation in Wistar rats

2015

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“…The detection of cPLA2 with an average molecular weight of 108 KDa using the WB technique is consistent with the work of Hirashima et al [27] in which a 110 kDa PLA2 was purified and characterized from bovine brain cytosol. Activation of cPLA2 is usually accompanied by cascade signaling and production of PGE2 as an inflammatory marker.…”

Section: Discussionsupporting

confidence: 89%

Comparative study on the independent and combined effects of omega-3 and vitamin B12 on phospholipids and phospholipase A2 as phospholipid hydrolyzing enzymes in PPA-treated rats as a model for autistic traits

et al. 2018

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BackgroundAbnormal phospholipid metabolism is a major component of many neurodevelopmental disorders including autism. Oral administration of propionic acid (PPA) can produce behavioral abnormalities and biochemical features in rodents similar to those observed in autism and can thus be used as a model to understand impaired brain fatty acid metabolism in autism.MethodsThe present study was designed to understand alterations in phospholipid metabolism in the brain of a rodent model of autism and to explore omega-3 and vitamin B12 as remedies. Five groups of rats were selected: Group 1 was the control. Group 2 was the rodent model of autism treated with a neurotoxic dose of PPA. Group 3 was given vitamin B12 cobalamin (16.7 mg/kg/day) for 30 days after PPA treatment. Group 4 was given pharmaceutical grade Omega-3 (200 mg cholesterol free-DHA/kg body weight/day), a product of Madre lab, Germany, for 30 days after PPA treatment for 3 days. Group 5 was given a combined dose of ω-3 + Vitamin B12 for the same duration post-PPA treatment. Phospholipid levels and Phospholipase A2 were measured in the brain homogenates of all the groups. ELISA and western blotting were used to detect the cPLA2 protein level.ResultsA significant decrease in phospholipid levels and a significant increase in cPLA2 were found in brain tissue of PPA-treated rats; however, both ω-3 and vitamin B12 were efficient in ameliorating the neurotoxic effect of PPA.ConclusionBoth ω-3 and vitamin B12 may play a role in ameliorating impaired phospholipid metabolism in autism; however, proper clinical trials are needed.

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“…The method has been described by our lab in previous studies [21]–[23]. Fatty acids were expressed as g/100 g total fatty acid.…”

Section: Methodsmentioning

confidence: 99%

“…We have extensively demonstrated that folic acid; vitamin B 12 and omega-3 fatty acids are interlinked in the one carbon cycle and the adverse effects of a maternal micronutrient deficient/imbalanced diet on the fatty acid metabolism in the liver and placenta could be ameliorated by the supplementation of omega-3 fatty acids during pregnancy [22], [23]. Taking these studies further we hypothesize that the expression of transcription factors in the liver of the offspring will be determined by the maternal micronutrients through one carbon cycle and the omega-3 fatty acid status.…”

Section: Introductionmentioning

confidence: 99%

Differential Regulation of Hepatic Transcription Factors in the Wistar Rat Offspring Born to Dams Fed Folic Acid, Vitamin B12 Deficient Diets and Supplemented with Omega-3 Fatty Acids

Meher

Joshi

2014

PLoS ONE

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Nutritional status of the mother is known to influence various metabolic adaptations required for optimal fetal development. These may be mediated by transcription factors like peroxisome proliferator activated receptors (PPARs), which are activated by long chain polyunsaturated fatty acids. The objective of the current study was to examine the expression of different hepatic transcription factors and the levels of global methylation in the liver of the offspring born to dams fed micronutrient deficient (folic acid and vitamin B12) diets and supplemented with omega-3 fatty acids. Female rats were divided into five groups (n = 8/group) as follows; control, folic acid deficient (FD), vitamin B12 deficient (BD) and omega-3 fatty acid supplemented groups (FDO and BDO). Diets were given starting from pre-conception and continued throughout pregnancy and lactation. Pups were dissected at the end of lactation. Liver tissues were removed; snap frozen and stored at −80°C. Maternal micronutrients deficiency resulted in lower (p<0.05) levels of pup liver docosahexaenoic acid (DHA) and arachidonic acid (ARA) as compared to the control group. Pup liver PPARα and PPARγ expression was lower (p<0.05) in the BD group although there were no differences in the expression of SREBP-1c, LXRα and RXRα expression. Omega-3 fatty acids supplementation to this group normalized (p<0.05) levels of both PPARα and PPARγ but reduced (p<0.05) SREBP-1c, LXRα and RXRα expression. There was no change in any of the transcription factors in the pup liver in the FD group. Omega-3 fatty acids supplementation to this group reduced (p<0.05) PPARα, SREBP-1c and RXRα expression. Pup liver global methylation levels were higher (p<0.01) in both the micronutrients deficient groups and could be normalized (p<0.05) by omega-3 fatty acid supplementation. Our novel findings suggest a role for omega-3 fatty acids in the one carbon cycle in influencing the hepatic expression of transcription factors in the offspring.

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Effect of maternal micronutrients (folic acid, vitamin B12) and omega 3 fatty acids on liver fatty acid desaturases and transport proteins in Wistar rats

Cited by 33 publications

References 55 publications

Heating of vegetable oils influences the activity of enzymes participating in arachidonic acid formation in Wistar rats

Heating of vegetable oils influences the activity of enzymes participating in arachidonic acid formation in Wistar rats

Comparative study on the independent and combined effects of omega-3 and vitamin B12 on phospholipids and phospholipase A2 as phospholipid hydrolyzing enzymes in PPA-treated rats as a model for autistic traits

Differential Regulation of Hepatic Transcription Factors in the Wistar Rat Offspring Born to Dams Fed Folic Acid, Vitamin B12 Deficient Diets and Supplemented with Omega-3 Fatty Acids

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