Comparative study on the independent and combined effects of omega-3 and vitamin B12 on phospholipids and phospholipase A2 as phospholipid hydrolyzing enzymes in PPA-treated rats as a model for autistic traits

Alfawaz, Hanan; Bhat, Ramesa Shafi; Al-Mutairi, Manar; Alnakhli, Osima M.; Al-Dbass, Abeer; Alonazi, Mona; Al-Mrshoud, Majidh; Hasan, Iman H.; El‐Ansary, Afaf

doi:10.1186/s12944-018-0850-1

Cited by 10 publications

(4 citation statements)

References 47 publications

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“…The finding of the current study is of great support to our previous work in which vitamin D, CoQ10, vitamin B12, carnitine, and bioflavonoids (as major components of bee pollen) demonstrate significant potency in ameliorating PPA-induced persistent autistic features in the rodent model [ 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ].…”

Section: Discussionsupporting

confidence: 88%

Efficacy of Novavit in ameliorating the neurotoxicity of propionic acid

Bukhari

Alfawaz

Al-Dbass

et al. 2020

Translational Neuroscience

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AbstractOxidative stress, abnormal fatty acid metabolism, and impaired gut microbiota play a serious role in the pathology of autism. The use of dietary supplements to improve the core symptoms of autism is a common therapeutic strategy. The present study analyzed the effects of oral supplementation with Novavit, a multi-ingredient supplement, on ameliorating oxidative stress and impaired lipid metabolism in a propionic acid (PPA)-induced rodent model of autism. Male western albino rats were divided into three groups. The first group is the control, the second group was given an oral neurotoxic dose of PPA (250 mg/kg body weight/day) for 3 days and then received buffered saline until the end of the experiment. The third group received Novavit (70 mg/kg body weight/day for 30 days after the 3-day PPA treatment). Markers of oxidative stress and impaired fatty acid metabolism were measured in brain homogenates obtained from each group. Novavit modulation of the gut microbiota was also evaluated. While PPA induced significant increases in lipid peroxides and 5-lipoxygenase, together with significantly decreased glutathione, and cyclooxygenase 2, oral supplementation with Novavit ameliorated PPA-induced oxidative stress and impaired fatty acid metabolism. Our results showed that the presence of multivitamins, coenzyme Q10, minerals, and colostrum, the major components of Novavit, protects against PPA-induced neurotoxicity.

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Section: Discussionsupporting

confidence: 88%

Efficacy of Novavit in ameliorating the neurotoxicity of propionic acid

Bukhari

Alfawaz

Al-Dbass

et al. 2020

Translational Neuroscience

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“…Approximately 23% of the body’s cholesterol is found in the CNS, and it is an important raw material for the growth and differentiation of neurons. Moreover, metabolic dysregulation characterized by reduced phospholipid levels in the brain and plasma has been implicated in ASD pathogenesis ( Alfawaz et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Construction of an immune-related ceRNA network to screen for potential diagnostic markers for autism spectrum disorder

Sun

Chen²,

Chen³

2022

Front. Genet.

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Purpose: The diagnosis of autism spectrum disorder (ASD) is reliant on evaluation of patients’ behavior. We screened the potential diagnostic and therapeutic targets of ASD through bioinformatics analysis.Methods: Four ASD-related datasets were downloaded from the Gene Expression Omnibus database. The “limma” package was employed to analyze differentially expressed messenger (m)RNAs, long non-coding (lnc)RNAs, and micro (mi)RNAs between ASD patients and healthy volunteers (HVs). We constructed a competing endogenous-RNA (ceRNA) network. Enrichment analyses of key genes were undertaken using the Gene Ontology database and Kyoto Encyclopedia of Genes and Genomes database. The ImmucellAI database was used to analyze differences in immune-cell infiltration (ICI) in ASD and HV samples. Synthetic analyses of the ceRNA network and ICI was done to obtain a diagnostic model using LASSO regression analysis. Analyses of receiver operating characteristic (ROC) curves were done for model verification.Results: The ceRNA network comprised 49 lncRNAs, 30 miRNAs, and 236 mRNAs. mRNAs were associated with 41 cellular components, 208 biological processes, 39 molecular functions, and 35 regulatory signaling pathways. Significant differences in the abundance of 10 immune-cell species between ASD patients and HVs were noted. Using the ceRNA network and ICI results, we constructed a diagnostic model comprising five immune cell-associated genes: adenosine triphosphate-binding cassette transporter A1 (ABCA1), DiGeorge syndrome critical region 2 (DGCR2), glucose-fructose oxidoreductase structural domain gene 1 (GFOD1), glutaredoxin (GLRX), and SEC16 homolog A (SEC16A). The diagnostic performance of our model was revealed by an area under the ROC curve of 0.923. Model verification was done using the validation dataset and serum samples of patients.Conclusion:ABCA1, DGCR2, GFOD1, GLRX, and SEC16A could be diagnostic biomarkers and therapeutic targets for ASD.

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“…Omega -3 dose (200 mg/kg/day) for 30 days tested in Juvenile Rats (80-120g) after treated with propionic acid. Vit B12 (16.7 mg/kg/day) treated for 30 days after propionic acid and combination of Omega-3 and Vit B12 [97], [99]. They results showed that these supplementations reduce oxidative stress and combination inhibits clostridia growth they suggested taking this combination as therapeutic treatment.…”

Section: Supplements and Antioxidant Used To Ameliorate Or Prevent Th...mentioning

confidence: 95%

Is Propionic acid a suitable model for autism?

Elmalahy¹,

Ali

Almaaty

et al. 2021

Alfarama Journal of Basic & Applied Sciences

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Autism Spectrum Disorder (ASD) is defined as different degrees of disability in social interaction, stereotyped behavior, and altered motor and sensory perception. Many scientific research are concerned with making and developing animal models of autism to figure out the appropriate correlation between behavioural changes and the pathology of brain tissue to help in clinical trials of effective therapeutic formulations. Propionic acid can be present inside the cells as metabolite in a normal state and present in high amount in the gut due to some digestive disorders or alteration in gut microbiota. Moreover, it is considered an artificial food additive. Lately, propionic acid has become a controversy model. This review assessment demonstrates a complete picture and all the available information is needed to be known about the mechanism of action of the induced autistic model. It discusses the different methods explains the pathophysiology, and various treatment modalities were tried in the propionic induced animal model. It also enlists the developmental and behavioral aspects of the model animals

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Comparative study on the independent and combined effects of omega-3 and vitamin B12 on phospholipids and phospholipase A2 as phospholipid hydrolyzing enzymes in PPA-treated rats as a model for autistic traits

Cited by 10 publications

References 47 publications

Efficacy of Novavit in ameliorating the neurotoxicity of propionic acid

Efficacy of Novavit in ameliorating the neurotoxicity of propionic acid

Construction of an immune-related ceRNA network to screen for potential diagnostic markers for autism spectrum disorder

Is Propionic acid a suitable model for autism?

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