Heating of vegetable oils influences the activity of enzymes participating in arachidonic acid formation in Wistar rats

Stawarska, Agnieszka; Białek, Agnieszka; Tokarz, A.

doi:10.1016/j.nutres.2015.05.022

Cited by 9 publications

(6 citation statements)

References 28 publications

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“…Therefore, we expected the highest contents of arachidonic and linoleic acids in the groups supplemented with oils richest in linoleic acid-sunflower oil, followed by sesame and rice oils, i.e., the oils we have been particularly focused on. However, not entirely in line with our expectations and our previous studies [33,34], the highest arachidonic acid level was found in the rice oil-fed group. This value was significantly increased than in the carotino oil-supplemented group and was followed by the group fed with sesame oil.…”

Section: Discussionsupporting

confidence: 74%

“…The vegetable oils used in the study differ in the content of individual fatty acids as well as in the proportions of n-6 and n-3 PUFA. Some authors pointed out that this relationship may have a direct impact on the fatty acid profile in the blood serum [33,34,39].…”

Section: Discussionmentioning

confidence: 99%

“…The product to precursor FA ratios were calculated to estimate the activities of desaturases [32][33][34]. The index of ∆6-desaturase (D6D) activity was determined as the ratio of γ-linolenic acid concentration (GLA, C18:3, n-6) to the linoleic acid (LA, C18:2, n-6) concentration in serum.…”

Section: Estimation Of Desaturases Activitymentioning

confidence: 99%

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Oils’ Impact on Comprehensive Fatty Acid Analysis and Their Metabolites in Rats

et al. 2020

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Fatty acids, especially polyunsaturated, and their metabolites (eicosanoids) play many pivotal roles in human body, influencing various physiological and pathological processes. The aim of the study was to evaluate the effect of supplementation with edible oils diverse in terms of fatty acid composition on fatty acid contents, activities of converting their enzymes, and on lipoxygenase metabolites of arachidonic and linoleic acids (eicosanoids) in rat serum. Female Sprague-Dawley rats divided into seven groups were used in the study. Animals from six groups were fed one of oils daily (carotino oil, made up by combining of red palm oil and canola oil, linseed oil, olive oil, rice oil, sesame oil, or sunflower oil). One group received a standard diet only. Fatty acids were determined using gas chromatography with flame ionization detection. Eicosanoids—hydroxyeicosatetraenoic (HETE) and hydroxyoctadecadienoic acids (HODE) were extracted using a solid-phase extraction method and analyzed with HPLC. Vegetable oils given daily to rats caused significant changes in serum fatty acid profile and eicosanoid concentrations. Significant differences were also found in desaturases’ activity, with the linseed and olive oil supplemented groups characterized by the highest D6D and D5D activity. These findings may play a significant role in various pathological states.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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Oils’ Impact on Comprehensive Fatty Acid Analysis and Their Metabolites in Rats

et al. 2020

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“…However, with the 20% increase of this oil, a difference was evident. From obtained results, we are inclined to believe that Wistar rats have the ability to elongate and desaturate PUFA [ 47 , 48 , 49 ]. Soybean oil contains an ALA precursor without DHA and EPA derivatives.…”

Section: Discussionmentioning

confidence: 99%

Benefits of Fish Oil Consumption over Other Sources of Lipids on Metabolic Parameters in Obese Rats

et al. 2018

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This study evaluated the effect of the consumption of different levels and sources of lipids on metabolic parameters of Wistar rats. Animals were fed with high-fat diet (HFD) containing 20% of lard for 12 weeks to cause metabolic obesity. Subsequently, the animals were divided into six groups and were fed diets with lipid concentrations of 5% or 20% of lard (LD), soybean oil (SO) or fish oil (FO), for 4 weeks. Data were submitted to analysis of variance (two-way) followed by Tukey post hoc test (p < 0.05). The groups that consumed FO showed less weight gain and lower serum levels of triacylglycerol (TAG), total cholesterol and fractions, aspartate aminotransferase (AST) activity, atherogenic index, less amount of fat in the carcass, decreased Lee index and lower total leukocyte counting (p < 0.05). These same parameters were higher in LD treatment (p < 0.05). In the concentration of 20%, carcass fat content, blood glucose levels, as well as alanine aminotransferase (ALT) and gamma glutamyl transferase (GGT) decreased in FO groups (p < 0.05). The SO group had intermediate results regarding the other two treatments (FO and LD). We concluded that fish oil intake was able to modulate positively the metabolic changes resulting from HFD.

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“…Livers were excised during autopsy and hepatic microsomes were prepared according to the method of Kłyszejko-Stefanowicz with slight modifications, which was thoroughly described previously [ 42 ]. Three parallel samples were prepared from liver sample obtained from one animal and they were stored frozen at −80 °C prior to analyses.…”

Section: Methodsmentioning

confidence: 99%

Lipidomic Profile and Enzymes Activity in Hepatic Microsomes of Rats in Physiological and Pathological Conditions

Lepionka

Białek

Czauderna

et al. 2021

IJMS

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Among the risk factors affecting the development of cancer, nutritional factors occupy a significant place. Pomegranate seed oil (PSO) and bitter melon extract (BME), used for ages in folk medicine, are nowadays used in the prevention of many diseases and as ingredients of dietary supplements. Despite numerous publications on these raw materials or their active substances, their mechanism of action in various pathological states has not been recognized yet, nor has the safety of their simultaneous use been evaluated. The study aimed to assess how dietary supplementation with either PSO, with BME, or both, affects fatty acids’ profiles and their metabolism in hepatic microsomes, as well as the activity of selected microsomal enzymes (COX-2 and CYP1B1). Experimental animals (Sprague-Dawley rats) were divided into eight parallel experimental groups, differing in applied dietary modifications (control, PSO, BME and both PSO and BME) and introduction of chemical carcinogen—7,12-dimethylbenz[a]nthracene. Obtained results indicated the pronounced effect of the cancerous process on lipid metabolism and demonstrated the antagonistic effect of applied dietary supplements on the content of individual fatty acids and the activity of CYP1B1 and COX-2. The applied broad analytical approach and chemometric data analysis confirmed that raw materials, for which potential cancer prevention has been previously demonstrated, may differ in effects depending on the coexisting pathological state.

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Heating of vegetable oils influences the activity of enzymes participating in arachidonic acid formation in Wistar rats

Cited by 9 publications

References 28 publications

Oils’ Impact on Comprehensive Fatty Acid Analysis and Their Metabolites in Rats

Oils’ Impact on Comprehensive Fatty Acid Analysis and Their Metabolites in Rats

Benefits of Fish Oil Consumption over Other Sources of Lipids on Metabolic Parameters in Obese Rats

Lipidomic Profile and Enzymes Activity in Hepatic Microsomes of Rats in Physiological and Pathological Conditions

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