Effect of Magnesium, Strontium or Fluoride Ions on in vitro Activities of Odontoblast-like Cells (MDPC-23)

Abstract

Magnesium (Mg), strontium (Sr) or fluoride (F) ions have been shown to affect in vitro activities of bone cells and in vivo mineralization. The purpose of this study was to investigate the effect of Mg, Sr and F ions on the activities of odontoblast-like cells (MDPC-23) using an in vitro cell culture model. Mg, Sr or F ions were added to the medium in the following concentrations: Mg -1, 5, 10 mM; Sr -0.05, 0.1, 0.3, 1 mM; F-0.005, 0.01, 0.05, 0.1 mM. Results were observed after 1, 7 and 14 days using Von Kossa staining, scanning and transmission electron microscopy. Preliminary results showed the following: Mg ions demonstrated a positive effect at low concentrations and a negative effect at high concentrations; Sr ions demonstrated a positive dose dependent effect up to a concentration of 0.1 mM and a negative effect at higher concentrations; and F ions demonstrated a positive dose-dependent effect up to a concentration of 0.005 mM and inhibitory effects at higher concentrations. This preliminary study showed that the positive or negative effects of Mg, Sr or F ions on odontoblastic activities (proliferation and mineralization) depend on their concentration levels in the environment.

Human treated dentin matrices combined with Zn-doped, Mg-based bioceramic scaffolds and human dental pulp stem cells towards targeted dentin regeneration

“…Magnesium (Mg) is naturally present in mineralized tissues, with a content of approximately 1% (w/w) in dentin and 0.5% (w/w) in enamel, cementum and bone [23]. Mg 2+ ions have a positive dose-dependent effect on the mineralization processes of odontoblasts up to a concentration of 1 mM, while higher concentrations delay these processes [24]. Mg-containing ceramic scaffolds have been already used for bone regeneration favoring osteogenic differentiation of MSCs and leading to new bone formation [25].…”

The effect of Mg–Ca–Sr alloy degradation products on human mesenchymal stem cells

“…In addition to different alloy extracts, Sr‐only extracts were included, to determine potential differences between Sr‐only and Mg–Ca–Sr co‐administration. Two different concentrations of the Sr compound was selected for testing, one corresponding to expected Sr amounts eluded from the alloy, and one concentration in the order of magnitude shown in literature to affect bone cell expression . In the following sections, results are presented by Mg/Ca/Sr content (expressed in mM), as well as extract numbers, as denoted in Table , for ease of navigation.…”

“…Forward Reverse magnitude shown in literature to affect bone cell expression. 13,28 In the following sections, results are presented by Mg/Ca/Sr content (expressed in mM), as well as extract numbers, as denoted in Table II, for ease of navigation.…”

Effect of Fluoride-substituted Apatite on In Vivo Bone Formation

“…Our preliminary studies on the effects of F À ions on the in vitro activities of odontoblast-like and osteoblast-like cells showed that mineralization was promoted at low F concentration in a dose-dependent manner [12,13]. Moreover, our previous work demonstrated that FAp with low F concentration (0.…”