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Up to date, tissue regeneration of large bone defects is a clinical challenge under exhaustive study. Nowadays, the most common clinical solutions concerning bone regeneration involve systems based on human or bovine tissues, which suffer from drawbacks like antigenicity, complex processing, low osteoinductivity, rapid resorption and minimal acceleration of tissue regeneration. This work thus addresses the development of nanofibrous synthetic scaffolds of polycaprolactone (PCL)-a long-term degradation polyester-compounded with hydroxyapatite (HA) and variable concentrations of ZnO as alternative solutions for accelerated bone tissue regeneration in applications requiring mid-and long-term resorption. In vitro cell response of human fetal osteoblasts as well as antibacterial activity against Staphylococcus aureus of PCL:HA:ZnO and PCL:ZnO scaffolds were here evaluated. Furthermore, the effect of ZnO nanostructures at different concentrations on in vitro degradation of PCL electrospun scaffolds was analyzed. The results proved that higher concentrations ZnO may induce early mineralization, as indicated by high alkaline phosphatase activity levels, cell proliferation assays and positive Alizarin-RedS stained calcium deposits. Moreover, all PCL:ZnO scaffolds particularly showed antibacterial activity against S. aureus which may be attributed to release of Zn 2+ ions. Additionally, results here obtained showed a variable PCL degradation rate as a function of ZnO concentration. Therefore, this work suggests that our PCL:ZnO scaffolds may be promising and competitive short-, mid-and long-term resorption systems against current clinical solutions for bone tissue regeneration.

Materials and methods:·We performed LOR analysis in DNAs from 80 paired of normal and HNSCC tissues, by designing a microsatellit~marker on chromosome 4q35.l, which specifically detects allelic loss of ING2 locus. TP53 mutation analysis and its relationship with ING2 chromosomal deletion were also performed in available 68 of the samples. The correlation between LOR status and clinicopathological

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Crystallographic Data and X-ray Structure Analysis of 13a. Crystals of 13a are triclinic, space group PI: a -10.91 (1) b = 9.570 (3), c = 8.758 (8) A; a = 65.15 (1), ß = 66.94 (3), y = 71.24 (1)°; Z = 2; dc = 1.21 g cm"3. Intensities were measured by using Mo Ka radiation [Philips PW1100 diffractometer, graphite monochromator, 6-29 scan mode, 3 < 6 < 25°, scan speed 0.025°s "1, scan width deg 1.7 + 0.6 tan 9], The total number of independent reflexions measured was 2384. The structure was solved by direct methods31 and refined by full-matrix least-squares32 to a final R-factor of 4.8%. In the refinements 1934 reflections with intensities greater than the standard deviation from counting statistics were used. Hydrogen atoms were found from difference Fourier syntheses. In the last cycles 263 parameters were refined (scale factor, extinction parameter, positional

Ameloblastoma is the most frequently encountered odontogenic tumor, characterized by a locally invasive behavior, frequent recurrences, and, although rare, metastatic capacity. Loss or inactivation of tumor suppressor genes (TSGs) allows cells to acquire neoplastic growth. The ING family proteins are tumor suppressors that physically and functionally interact with p53 to perform important roles in apoptosis, DNA repair, cell cycle regulation, and senescence. TP53 genetic alterations were reported to infrequently occur in ameloblastoma. Considering that other TSGs related to TP53 could be altered in this tumor, we focused our study on the ING family genes. We analyzed the loss of heterozygosity (LOH) status of the ING family (ING1-ING5) chromosomal loci in a group of ameloblastomas by microsatellite analysis, and correlated the ING LOH status with clinicopathological characteristics. By using specific microsatellite markers, high frequency of LOH was found at the loci of each ING gene family member (33.3-72.2%). A significant relationship was shown between LOH of D2S 140 (ING5 locus) and solid tumor type (p = 0.02). LOH of ING3MS (ING3 locus) was also high in solid type tumors, showing a near significant association. In addition, a notable tendency toward higher LOH for half of the markers was observed in recurrent cases. LOH of ING family genes appears as a common genetic alteration in solid ameloblastoma. The current study provides interesting novel information regarding the potential prognostic significance of the allelic loss of the ING gene family loci in ameloblastoma tumorigenesis.

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