Effect of Levothyroxine on Total Lipid Profiles as Assessed by Analytical Capillary Isotachophoresis in a Patient with Hypothyroidism

Abstract

Abstract. The patient was a 51-year-old Japanese female who had been diagnosed with hyperlipidemia. At the first medical examination, her serum levels of total cholesterol (TC) and triglyceride (TG) were 482 and 205 mg/dl, respectively. Since hyperlipidemia was not improved by pravastatin, atorvastatin or niceritrol, and since the levels of thyroid-stimulating hormone (TSH) and free T4 were 730 IU/ml and 0.3 ng/dl, respectively, the patient was diagnosed as secondary hyperlipidemia with hypothyroidism. A method for the charge isolation of lipoproteins using capillary isotachophoresis (cITP) is proposed as a clinical application because it allows us to quantitatively measure electronegative low-density lipoprotein cholesterol (LDL-C), a potent marker of coronary heart disease. After 5 months of treatment with levothyroxine, Serum TC and LDL-C levels drastically decreased without statin treatment and high-density lipoprotein cholesterol (HDL-C) increased. In the lipoprotein profiles as assessed by cITP after treatment with levothyroxin, all HDL-C subfractions were increased and fast-migrating LDL/electronegative LDL appeared to be greatly reduced after treatment, while the area under the non-modified LDL peak was increased. The cITP analysis was able to obtain more information about coronary risk factors and may be clinically useful for evaluating the effect of treatment with levothyroxine in patients with hypothyroidism and secondary hyperlipidemia.

Supplementation of curcumin and vitamin E enhances oxidative stress, but restores hepatic histoarchitecture in hypothyroid rats

“…Various reports suggest that administration of T 3 or T 4 (levothyroxine) to hypothyroid rats restores the change in lipid profile (Ruggiero et al, 1987;Kiya et al, 2006). However, Mogulkoc et al (2005a,b) reported that thyroxine administration to hypothyroid rats resulted in oxidative stress in several tissues.…”

Myxedema Ascites with an Extremely Elevated CA125 Level: A Case Report

“…Because the screening test is simple, the disease can be detected in its early stage with dyslipidemia [1]. Canaris et al showed that only 50% of newly diagnosed hypothyroid patients had symptoms [2].…”

Recent progress in analytical capillary ITP

“…A study of the effects of atorvastatin and apoA‐I/phosphatidylcholine discs has shown a decrease in triglyceride‐rich lipoprotein subfractions, suggesting that their combination could be a promising therapeutic approach for hypertriglyceridemia 39. Other works examined the effects of apolipoprotein A‐I/phosphatidylcholine discs on charge‐based low‐density lipoprotein (LDL) subfractions 40, the relation between the remnant‐like particles‐cholesterol level and charge‐based apolipoprotein B‐containing lipoprotein subfractions in patients with hypercholesterolemia 41, extracellular‐signal‐regulated kinase 1/2 activity using LDL with various degrees of oxidation 42, the mechanism by which apolipoprotein A‐I/phosphatidylcholine discs increase pre‐β high‐density lipoproteins 43, the association between the level of fast‐migrating LDL subfraction and carotid‐artery intima‐media thickness 44, the effects of cholesterol feeding and simvastatin treatment on plasma platelet‐activating factor acetylhydrolyse activity 45, the association between atrial fibrillation and platelet‐activating factor acetylhydrolase 46 and the effect of levothyroxine on total lipid profiles in a patient with hypothyroidism 47. ITP was used for the determination of acetic acid (HAc) in the intestinal contents from jejunum, ileum and colon (LE: 10 mM HCl, 22 mM 6‐amino‐ n ‐hexanoic acid (EACA), 0.1% methylhydroxyethylcellulose (MHEC); TE: 5 mM caproic acid) 48.…”