Effect of lansoprazole on human sperm motility, sperm viability, seminal nitric oxide production, and seminal calcium chelation

Abstract

Lansoprazole is a proton-pump inhibitor that is commonly used to treat many gastric illnesses. However, little is known about its effect on sperm function. Here, we investigated the in vitro effect of LP on human sperm motility, viability, nitric oxide (NO) production, and the ability of LP to chelate seminal calcium. Seventy-two semen samples from normozoospermic men were tested in this study. The effects of LP at 0.375, 0.75, 1.5, and 3 μg/mL on sperm motility and viability as well as at 3 μg/mL on NO production and calcium chelation in semen were assessed. Lansoprazole at 3 μg/mL significantly decreased total and progressive sperm motility (P = 0.0021, P = 0.0256, respectively), but not sperm viability (P = 0.8763). In addition, semen samples supplemented with 3 μg/mL LP had insignificant changes (P = 0.9085) in nitrite concentrations. Moreover, LP exhibited a significant (P < 0.0001) calcium chelation effect in semen. In conclusion, LP reduced sperm motility, but not viability. The reduction in sperm motility may be due to the calcium chelating effect of LP and/or decreased Na+/K+-ATPase activity, but not an alteration in NO production. Besides, none of the tested parameters was found to be correlated with male age.

Effect of lansoprazole on DNA integrity of human spermatozoa and activity of seminal creatine kinase

“…In the present study, we asked whether lansoprazole affects the DNA integrity of human spermatozoa. As assessed by flow cytometry, we did not observe any significant influence of lansoprazole at 3 µg/ml on sperm DNA integrity, even though lansoprazole at this particular concentration has been documented to reduce sperm function (Banihani & Khasawneh, 2018). Accordingly, it can be suggested that the observed negative effect of lansoprazole on sperm function (Banihani & Khasawneh, 2018), particularly sperm motility, may not be modulated by increased level of DNA damage.…”

“…This evidence proposes that the use of lansoprazole to treat acid‐related gastrointestinal disorders may inadvertently increase production of reactive oxygen species and consequently formation of oxidative stress (an imbalance between oxidants and antioxidants (Alzoubi et al, 2018; Hani & Bayachou, 2014)) in the testicles, a reason may negatively affect semen quality. Accordingly, we have recently published an in vitro study on human semen ejaculates to assess sperm function in presence of lansoprazole at a range of concentrations considering the estimated average levels of lansoprazole in plasma when given at the therapeutic doses (Banihani & Khasawneh, 2018). In this study, we have shown that lansoprazole at 3 µg/ml (~8.122 µM ) concentration significantly reduced sperm motility (Banihani & Khasawneh, 2018).…”

“…Accordingly, we have recently published an in vitro study on human semen ejaculates to assess sperm function in presence of lansoprazole at a range of concentrations considering the estimated average levels of lansoprazole in plasma when given at the therapeutic doses (Banihani & Khasawneh, 2018). In this study, we have shown that lansoprazole at 3 µg/ml (~8.122 µM ) concentration significantly reduced sperm motility (Banihani & Khasawneh, 2018).…”

Seminal plasma vitamin B 6 levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry

“…To achieve a better accuracy, 200 spermatozoa were assessed per each replicate. Scanned sperm fields were randomly selected to minimise the bias in the measurement, and sperm counting was performed very quickly to avoid positive errors in the calculated percentages (Banihani & Khasawneh, 2018).…”

“…versus total sperm count in men with asthenozoospermia (b, n = 97) compared to men with normal sperm motility (a, n = 88) vitamins, including vitamin B 6 , were found to have lower levels of oxidative stress, an imbalance between oxidants and antioxidants to the favour of oxidants (Banihani, 2018b;Hani & Bayachou, 2014;Mhaidat et al, 2015), demonstrating a direct antioxidant activity of such group of vitamins (Ullegaddi, Powers, & Gariballa, 2004). Accordingly, given that accumulation of ROS in semen may lead to oxidative stress (Banihani, 2018a), and consequently oxidative damage to spermatozoa (Banihani, 2016a(Banihani, , 2016bMayorga-Torres, Camargo, Cadavid, Plessis, & Cardona Maya, 2016), then vitamin B 6 , once present at normal levels in semen, should enhance the chemical defence mechanism and delay the progression of such oxidative injury, thereby maintains/protects sperm function, particularly sperm motility.…”