Current Insights and Latest Updates in Sperm Motility and Associated Applications in Assisted Reproduction

Dcunha, Reyon; Hussein, Reda S.; Ananda, Hanumappa; Kumari, Sandhya; Adiga, Satish Kumar; Kannan, Nagarajan; Zhao, Yulian; Kalthur, Guruprasad

doi:10.1007/s43032-020-00408-y

Cited by 68 publications

(48 citation statements)

References 218 publications

(205 reference statements)

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“…IPM had a very robust positive correlation (r=0.95), and MDA levels had a very robust negative correlation (r=−0.98) with sperm motility (Figures- 3 and 5). This result conforms to those of an earlier study where motility was an important parameter of male fertility [65]. The quality standard for post-thawed goat semen is a minimum of 40% sperm motility in 50 million sperm per dose for AI, according to the Indonesian National Standard Agency [10].…”

Section: )supporting

confidence: 89%

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

et al. 2021

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Background and Aim: Kacang buck sperm is cryosensitive due to the seminal plasma of semen itself. Meanwhile, bull seminal plasma contains the insulin-like growth factor-1 (IGF-1) complex, which is cryoprotective. The addition of the crude protein of Simmental bull seminal plasma increased the quality of post-thawed semen of Kacang buck. The study was conducted to determine the effects of Simmental bull seminal plasma with IGF-1 on the fertility of post-thawed Kacang buck semen. Materials and Methods: Buck semen was diluted in the following skim milk-egg yolk extender preparations: Without the addition of Simmental bull seminal plasma IGF-1 complex protein (T0); with the addition of 12-μg Simmental bull seminal plasma IGF-1 complex protein (T1); and with the addition of 24-μg Simmental bull seminal plasma IGF-1 complex protein (T2). The extended semen was packed in 0.25-mL straws and frozen. Post-thawed semen fertility was evaluated based on the following variables: Sperm motility, viability, intact plasma membrane (IPM), malondialdehyde (MDA) levels, capacitation status, and acrosome reaction. The difference in each variable among the groups was evaluated using analysis of variance, followed by Tukey's honestly significant difference test, at a 95% level of significance. Meanwhile, principal component analysis (PCA) was used to identify the principal component of semen fertility among the seven parameters. Results: The T1 group showed the highest sperm motility, viability, IPM, and percentage of incapacitated sperm and the lowest MDA levels, percentage of capacitated sperm, and acrosome reaction. PCA revealed that sperm motility had a moderate to very robust correlation with other variables and is the most crucial parameter, accounting for 80.79% of all variables. Conclusion: The IGF-1 complex in Simmental bull seminal plasma was useful for increasing the fertility of post-thawed Kacang buck semen, and sperm motility was the principal component of semen fertility.

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Section: )supporting

confidence: 89%

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

et al. 2021

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“…Furthermore, both GIV transcripts and its phosphoactivation by TKs (pYGIV) was reduced in sperms with lower motility. Because the global trend of progressive reduction in the number of motile and viable sperm in the ejaculate has been associated with a concomitant increase in the rates of infertility 63 , our findings in the case of GIV add to the growing number of proteins that enrich the signal-ome of healthy sperm. For example, as an intrinsically disordered protein (IDP) and a multi-modular scaffold that generates crosstalk between diverse signaling pathways, GIV appears to be in a prominent position to orchestrate rapid cooperativity between these pathways and processes in the otherwise transcriptionally and translationally silent sperm cell.…”

Section: Discussionmentioning

confidence: 82%

GIV/Girdin, a Non-receptor Modulator for Gαi/s, Regulates Spatiotemporal Signaling during Sperm Capacitation and is Required for Male Fertility

Reynoso

Castillo

Katkar

et al. 2021

Preprint

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For a sperm to successfully fertilize an egg, it must first undergo capacitation in the female reproductive tract, and later undergo acrosomal reaction (AR) upon encountering an egg surrounded by its vestment. How premature AR is avoided despite rapid surges in signaling cascades during capacitation remains unknown. Using a combination of KO mice and cell-penetrating peptides, we show that GIV (CCDC88A), a guanine nucleotide-exchange modulator (GEM) for trimeric GTPases, is highly expressed in spermatocytes and is required for male fertility. GIV is rapidly phosphoregulated on key tyrosine and serine residues in human and murine spermatozoa. These phosphomodifications enable GIV-GEM to orchestrate two distinct compartmentalized signaling programs in the sperm tail and head; in the tail, GIV enhances PI3K→Akt signals, sperm motility and survival, whereas in the head it inhibits cAMP surge and premature AR. Furthermore, GIV transcripts are downregulated in the testis and semen of infertile men. These findings exemplify the spatiotemporally segregated signaling programs that support sperm capacitation and shed light on a hitherto unforeseen cause of infertility in men.

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“…The interview was conducted to fill out the questionnaire with the gym-goers in the laboratory, as well as blood sampling, each participant needed approximately (15)(16)(17)(18)(19)(20) minutes to complete the data collection and blood sampling.…”

Section: Data Collection Methodmentioning

confidence: 99%

“…Recent advancements around infertility have had a significant impact on our perception of the various causes of male factor infertility. Though environmental, physiological, and genetic factors have been identified, misuse of anabolic-androgenic steroids (AASs) tends to affect sperm function and cause pregnancy delays [16].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Anabolic-Androgenic Steroids on Males’ Hormones Among Gym-Goers in Mosul City

Hashim¹,

Almukhtar²

2021

Proceedings of the 1st International Ninevah Conference on Medical Sciences (INCMS 2021)

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Anabolic-Androgenic Steroids (AAS) misuse are growing worldwide and misused especially among male bodybuilders and gyms-goers for building muscle to improve their physical appearance. AAS misuse is considered a preventable factor of male fertility because it affects the hypothalamic-pituitary-gonadal axis resulting in secondary male hypogonadism, and then transient or persistent suppression of spermatogenesis. Study aimed to investigate the influence of anabolic steroids misuse on the serum concentrations of follicle stimulating hormone, luteinizing hormone, and free testosterone. A prospective cohort study was conducted on 80 healthy male gym-goers divided into the case and control group who attending gyms in Mosul city. A structured interviewing questionnaire was used to collect information related to AAS misuse, blood sampling collected for evaluation of males' hormones in a private analytics Lab, for a period 25th October 2020 until 15th March 2021. Study finding shows that males' hormones of AAS group exhibited highly significant (P.>0.001) in the overall mean of serum concentrations of follicle stimulating hormone, luteinizing hormone, and free testosterone. ROC curve analysis reveals a highly significant deference at (P.>0.001) of males' hormones, which area under the curve was for follicle stimulating hormone: 0.94, luteinizing hormone: 0.93, and free testosterone: 0.81. The misuse of AAS exhibited a severe decrease in concentrations of gonadotropins and free testosterone with may be considered an excellent predictor of secondary male hypogonadism. The study recommends the necessity to prevent abuse of anabolic steroids and instruct about the adverse effects that result from using it and especially on fertility.

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Current Insights and Latest Updates in Sperm Motility and Associated Applications in Assisted Reproduction

Cited by 68 publications

References 218 publications

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

GIV/Girdin, a Non-receptor Modulator for Gαi/s, Regulates Spatiotemporal Signaling during Sperm Capacitation and is Required for Male Fertility

The Influence of Anabolic-Androgenic Steroids on Males’ Hormones Among Gym-Goers in Mosul City

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