Effect of inoculation route on the production of antibodies and histological characteristics of the spleen in laying hens

Abstract

Recent studies have reported the use of IgY antibody in the prevention or treatment of diseases in animals. IgY can be obtained in large amounts from the yolk of chicken eggs through a low-cost process. This study evaluated the effect of different routes of inoculation on antibody production and spleen morphological characteristics of laying hens (White Leghorn) inoculated with sheep red blood cells. The analysis of the results showed that the intramuscular route is the most efficient for total antibody production in the primary immune response, while the intravenous route is the most efficient in producing IgY antibodies in the secondary immune response. No histological changes were observed in the spleen of laying hens. This study could be useful for developing protocols of antigen inoculation in laying hens for IgY antibody production

Ativação de células de memória na produção de anticorpos e na expressão de células IgM positivas no baço de tilápias-do-nilo

“…http://dx.doi.org/10.1590/1678-4162-9063 geradas na expansão clonal, migram para a medula óssea e residem no tecido como células de memória (Eto et al, 2012).…”

Effects of inoculation route and dose on production and avidity of IgY antibodies

“…Chang et al (1999), using S. mutans to compare the effects of IM and SC routes, showed that the production of IgY via IM was almost 10 times greater than through SC. Furthermore Eto et al (2012), using sheep red blood cells as the antigen observed that the intravenous route resulted in a better response when compared with IM and SC, although there were no significant differences in the levels of antibodies produced by these two routes.…”

“…The most common routes for antigen inoculation are IM, SC, and ID routes Eto et al, 2012;Murphy, Tavares, & Walport, 2010). In laying hens, the IM route is the most commonly used for production of antibodies (Schade et al, 2005).…”

“…These studies show that the levels of production of IgY can be influenced by the inoculation route chosen and antigen dose (Eto et al, 2012;Schade et al, 2005;Schwarzkopf, Staak, Behn, & Erhard, 2000). For example, studies show that the subcutaneous (SC) route results in higher levels of antibodies than other routes, such as the intramuscular (IM) route, but this effect may be dependent on the type of antigen used (Chang, Ou-Yang, Chen, & Chen, 1999;Erhard et al, 2000).…”

Immunomodulatory action of jacalin from Artocarpus integrifolia and mannoprotein from Saccharomyces uvarum on the humoral immunity of laying hens

“…There is a great interest in the production of specific antibodies in laying hens for immunotherapy and immunodiagnostics to replace antibodies produced in mammalians (MONTINI et al, 2018;MARCQ et al, 2015;MARCQ et al, 2013;ETO et al, 2012). Laying hens represent an alternative to mammalians due to the possibility of obtaining large quantities of IgY from egg yolk at a low cost (MUNHOZ et al, 2014).…”