Ativação de células de memória na produção de anticorpos e na expressão de células IgM positivas no baço de tilápias-do-nilo

Production and use of immunoglobulin Y in the diagnosis of haemorrhagic septicemia caused by Aeromonas hydrophila in Piaractus mesopotamicus

“…The purified Ig Y Anti‐ A . hydrophila from the yolk showed two bands to the heavy chain 84 kDa and the light chain 28 kDa of the IgY molecular structure (Figure 1), which was similar to other molecular structures of immunoglobulin Y (Chacana et al, 2004; Eto et al, 2018, 2019). The results showed an increase ( p < 0.05) in the levels of IgY anti‐ A .…”

“…According to our study, the humoral immune system of birds appears to have the potential to produce specific antibodies against exotoxins and proteins of A. hydrophila. Previous studies showed similar results in Oreochromis niloticus and Rachycentron canadum challenged with S. agalactiae and P. damselae respectively (Eto et al, 2018(Eto et al, , 2019. The SDS-PAGE analysis of A. hydrophila showed a reaction in 27 bands of total fraction, the most notorious bands were of 80,43,40,38,34,16,15 and 10 kDa (Figure 4a).…”

“…hydrophila . Previous studies showed similar results in Oreochromis niloticus and Rachycentron canadum challenged with S. agalactiae and P. damselae respectively (Eto et al, 2018, 2019). The SDS‐PAGE analysis of A .…”

“…Immunoglobulin Y (IgY) is the main antibody produced by chickens Gallus domesticus (Silva & Tambourgi, 2010) and is continuously synthesized and excreted into the blood and transferred to the egg yolk, where it is accumulated (Eto et al, 2019; Silva & Tambourgi, 2010). Some studies reported the use of IgY as prophylactic and treatment for some fish pathogens such as Photobacterium damselae (Eto et al, 2019) and Streptococcus agalactiae (Eto et al, 2018). In addition, the production of IgY is relatively inexpensive and can be produced in large quantities and it is not necessary to sacrifice the hen to obtain it (Andrade et al, 2013; Eto et al, 2018, 2019).…”

“…Some studies reported the use of IgY as prophylactic and treatment for some fish pathogens such as Photobacterium damselae (Eto et al, 2019) and Streptococcus agalactiae (Eto et al, 2018). In addition, the production of IgY is relatively inexpensive and can be produced in large quantities and it is not necessary to sacrifice the hen to obtain it (Andrade et al, 2013; Eto et al, 2018, 2019).…”