“…This does not meet the expectation for diminished biohydrogenation as a result of lower pH (Talpur, 2007) in response to silage feeding (Jalč et al, 2013), but in the buffaloes this is confirmed. In the present study the values of stearic acid in the buffalo milk are higher in comparison not only to the bovine milk but also to other results for the Bulgarian Murrah (Naydenova, 2005;Mihaylova and Peeva, 2007) and other breeds Varricchio et al, 2007).…”
“…This does not meet the expectation for diminished biohydrogenation as a result of lower pH (Talpur, 2007) in response to silage feeding (Jalč et al, 2013), but in the buffaloes this is confirmed. In the present study the values of stearic acid in the buffalo milk are higher in comparison not only to the bovine milk but also to other results for the Bulgarian Murrah (Naydenova, 2005;Mihaylova and Peeva, 2007) and other breeds Varricchio et al, 2007).…”
“…VFA production initially was lower with silages compared with wilted forages up until 8 h of incubation and until 2 h of incubation, proportions of acetate were higher and propionate was lower in incubations with wilted compared with ensiled mixtures. Lactic acid in silages, which is quickly fermented (,25 min), mainly results in the formation of propionate (Jalc et al, 2009;Lima et al, 2010). The latter is in line with the lower CH 4 production, as an increased propionate formation can lead to a lower CH 4 excretion (Demeyer and Fievez, 2000) and has been reported before for ensiled forages (Lima et al, 2011).…”
Most often, farmers consider red clover an unattractive forage because of its low ensilability. Nevertheless, several in vivo and in vitro experiments also showed advantages of red clover silages such as decreased rumen biohydrogenation of polyunsaturated fatty acids. This has been attributed to a possible protective role of protein-bound phenols, with polyphenol oxidase playing a key role in their formation. This enzyme is active in red clover, but not in other green forages, such as, for example, perennial ryegrass. Therefore, the aim was to study the lipid metabolism within red clover/ryegrass mixtures in lab scale silages and during in vitro rumen batch incubations. Ensilability of red clover increased with higher proportions of ryegrass in the silage mixture. However, the lipid-protecting mechanism of red clover does not seem to occur in the co-ensiled ryegrass as lipolysis of polar lipids linearly increased with increasing proportions of ryegrass (86.0%, 91.6%, 89.9%, 93.1% and 95.6% in 60-day-old silages with 100/0, 75/25, 50/50, 25/75 and 0/100 red clover/ryegrass, respectively). Rumen lipolysis and biohydrogenation of C18:3n-3 and C18:2n-6 were negatively related to red clover proportions in the silage mixtures. The lipid-protective mechanism in red clover silages is confirmed, but it seems not to be transferred to lipids in co-ensiled forages.Keywords: lipid metabolism, red clover, in vitro rumen, silage
ImplicationsLipids in red clover silages are less degraded as compared with lipids in ryegrass silages, which have been attributed to a lipid-protective mechanism in red clover. This implicates that less oilseeds would be required to reach milk with a healthier fatty acid profile when feeding red clover instead of ryegrass silages. However, the lipid protection is not transferred to grass lipids in mixed grass-red clover silages.
“…At present, there is little data in the literature on combinations of the two supplements -EFE and microbial inoculantsas food additives to improve animal performance (Jalč et al, 2009). Addressing this, Jalč et al (2009) and Reddy et al (2016) have indicated that, although the use of EFE originating from fungi has been demonstrated to increase the nutritional value of poorquality feed, the use of the fungi themselves, rather than their enzymes, would be easier and more economical. Moreover, fungi are increasingly often used to improve feed quality, and thus directly affect the quality of ruminant products (Varadyova et al, 2018).…”
The supplementation of ruminant diets with exogenous cellulolytic enzymes can improve their digestibility and feeding value. The objective of this study was to determine the effect of supplementing roughage (rice straw) and concentrate with inoculants containing four fungal strains (Pleurotus ostreatus, Phanerochaete chrysosporium, Trichoderma reesei and Trichoderma viride) and four bacterial strains (Paenibacillus polymyxa, Bacillus megaterium, Bacillus circulans and Bacillus subtilis), given separately or as a mixture, as a source of exogenous cellulolytic enzymes, on basic rumen parameters in vitro, including digestibility and methane production. A batch culture trial was used to select the best supplements, and a long-term rumen simulation technique (RUSITEC) was used to evaluate the effects of P. chrysosporium, B. subtilis, and a 1 : 1 mixture of these two on dietary component digestibility and fermentation parameters. In the batch culture evaluation, there were significant increases in the organic matter (OM) digestibility, the total gas production expressed as ml/g of dry matter, the OM, the neutral detergent fibre (NDF) and the acid detergent fibre (ADF) of the supplemented rations, as compared to the control, excluding the rations supplemented with T. viride and B. circulans. In the RUSITEC, the ration supplemented with mixed inoculants showed significantly higher digestibility of crude protein, ether extract, NDF and ADF than did the ration supplemented with the P. chrysosporium and B. subtilis inoculants. It can be concluded that the simultaneous use of fungal and bacterial exogenous cellulases on rice straw roughage improves its digestibility, without negative effects on other rumen parameters.
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