The experiment has been carried out with 10 lactating buffaloes (Bulgarian Murrah breed) in an interval of two successive days. They were kept in shade(first day) and exposed to direct solar radiation (second day) with ambient temperature not less than 30 0 C for 12 h. Rectal temperature(RT) and respiratory rate (RR) were measured at 8 a.m., 11 a.m., 3 p.m. and 8 p.m. Both RT and RR increased significantly at temperature – humidity index (THI)- 77.83, showing that the lactating buffaloes are sensitive to heat stress and are not able to maintain their core temperature within the thermoneutral zone. The same THI had no significant effect on rectal temperature elevation when the buffaloes were kept in the barn. These data demonstrate that lactating buffaloes need protection against direct solar radiation. Plasma cholesterol and total protein levels tended to be lower during the exposure to direct solar radiation compared to the morning levels. Plasma urea level increased and plasma indol level remained unchanged when the buffaloes were exposed to sun. It is suggested that the level of the first three parameters is closely related with the dynamic of cortisol and blood volume fluctuation in animals under heat

No abstract

The objective of this study were 2 fold: to determine whether the weaning (abrupt transition from liquid to solid diet) at the age of 90 days causes stress in buffalo calves, and if so, to alleviate the negative effect of stress by chromium picolinate supplementation (400 μg daily). All buffalo calves were separated from their mothers immediately after birth. Chromium supplementation started 3 days before the weaning and was terminated 5 days later. Plasma cholesterol and indol levels declined significantly after weaning in both groups. Both the weaning and supplemental chromium had no effect on plasma urea level. Our results indicate that when the negative emotional arousal, caused by the interruption of the physical contact mother – calf, is avoided, as it is in our case, then the abrupt transition from liquid to solid feed at 90 d of age has no stress-inducing effect. Supplemental chromium reduced plasma cortisol levels both before and following the weaning, but had no significant effect on the determined metabolites since its effect is more pronounced during extreme stress load

This is the first study to explore the genetic diversity and population structure of domestic water buffalo (Bubalus bubalis) in Germany and their potential relations to herds in other parts of Europe or worldwide. To this end, animals from different herds in Germany, Bulgaria, Romania, and Hungary were genotyped and compared to genotypes from other populations with worldwide distribution and open to the public. The pilot study analyzed population structure, phylogenetic tree, and inbreeding events in our samples. In buffalos from Germany, a mixed genetic make-up with contributions from Bulgaria (Murrah breed), Romania, and Italy was found. All in all, a high degree of genetic diversity was identified in European buffalos, and a novel genotype was described in Hungarian buffalos by this study. We demonstrate that European buffalos stand out from other buffalo populations worldwide, supporting the idea that buffalos have not completely disappeared from the European continent during the late Pleistocene. The high genetic diversity in European buffalos seems to be an excellent prerequisite for the establishment of local breeds characterized by unique traits and features. This study may also be considered as an initial step on the way to genome characterization for the sustainable development of the buffalo economy in Germany and other parts of Europe in the future.

No abstract