Effect of indole-3-butyric acid on root formation in Alnus glutinosa microcuttings

José, Mario; Romero, Lourdes; Janeiro, Laura V.

doi:10.14214/sf.916

Cited by 18 publications

(5 citation statements)

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“…All developed roots, regardless of the medium on which they were induced and the way of formation (with callus and without callus) originated from the division region within the phloem tissue. In in vitro cultivated shoots of Malus, Alnus, Castanea and Cedrela the initial meristemoids of adventitious roots develop either within the phloem adjacent to cambium, at ray parenchyma or cortical and phloem parenchyma (Zhou et al 1992;Goncalves et al 1998;Naija et al 2008;Millán-Orozco et al 2011;San José et al 2012). Mitotic activity is also observed within the core.…”

Section: Discussionmentioning

confidence: 99%

Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Wiszniewska

Nowak

Kołton

et al. 2016

Plant Cell Tiss Organ Cult

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The study aimed at evaluating the response of common plum (Prunus domestica L.) microshoots during in vitro rooting in the presence of two phytoactive medium supplements, i.e. a dialyzate of pineapple pulp and a conditioned medium containing green algae Desmodesmus subspicatus exudates. Rooting efficiency was evaluated after 4 weeks of culture. During the root induction phase the content of phenolic compounds in shoot bases was determined and anatomical studies were conducted. Medium supplements were analyzed for the content of carbohydrates and phenolic acids. Both supplements were efficient in rooting induction of shoots of a difficult-to-root cultivar 'Węgierka Zwykła'. Medium supplementation allowed a significant reduction in the exogenous auxin content required for rooting. The highest rooting efficiencies on supplemented media were 28.9 and 27.8 %, in comparison with 33.3 % obtained in the control medium with doubled concentration of exogenous auxins. In the easy-to-root plum cultivar 'Węgierka Dąbrowicka' the rooting rate was slightly reduced in the presence of pineapple dialyzate, while in the presence of algal conditioned medium the rooting rate decreased substantially compared with the nonsupplemented medium. Approximately 30 % of 'Węgierka Dąbrowicka' shoots rooted on supplemented auxin-free media. The content of phenolic compounds accumulated in shoot bases during the root induction stage reflected the differences in rooting ability between both plum cultivars, indicating potential stressful conditions of the culture generated by the presence of phytoactive natural supplements. Anatomical study allowed to recognize the mode of dedifferentiation leading to adventitious rhizogenesis in the common plum. The results are discussed in relation to the composition of medium supplements and their potential root-promoting activity.

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Section: Discussionmentioning

confidence: 99%

Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Wiszniewska

Nowak

Kołton

et al. 2016

Plant Cell Tiss Organ Cult

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“…On the hormone-free medium, IBL 91/78 clearly surpassed H275 in both rooting rate (Figure 1b) and the number of roots per explant (Figure 2a). Auxin is the most widely reported hormonal inducer of adventitious root formation both in Populus [43] and in other plant species [44,45]. Also, the positive role of auxin in the promotion of shoot elongation is well reported in Arabidopsis [46], and confirmed in Populus [47].…”

Section: Analysis Of Populus Genotype Responses To the Darkness And G...mentioning

confidence: 90%

Differences in Environmental and Hormonal Regulation of Growth Responses in Two Highly Productive Hybrid <em>Populus</em> Genotypes

Žiauka¹,

Striganavičiūtė²,

Szyp-Borowska³

et al. 2021

Preprint

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Phenotypic plasticity in response to adverse conditions determines plant productivity and survival. The aim of this study was to test if two highly productive Populus genotypes, characterized by different in vitro etiolation patterns, differ also in their responses to hormones gibberellin (GA) and abscisic acid (ABA), and to a GA biosynthesis inhibitor paclobutrazol (PBZ). The experiments on shoot cultures of ‘Hybrida 275’ (abbr. H275; Populus maximowiczii × P. trichocarpa) and IBL 91/78 (Populus tremula × P. alba) were conducted either by modulating the physical in vitro environment or by adding specific chemicals to the nutrient medium. Our results show that there are significant differences between the studied genotypes in environmental and hormonal regulation of growth responses. The genotype H275, which responded to darkness with PBZ-inhibitable shoot elongation, was unable to recover its growth after treatment with ABA. In contrast, the genotype IBL 91/78, whose shoot elongation was not affected either by darkness or PBZ treatment, recovered so well after the ABA treatment that, when rooted subsequently, it developed longer shoots and roots than without ABA treatment. Our results indicate that GA catabolism and repressive signaling provide an important pathway to control growth and physiological adaptation in response to immediate or impending adverse conditions. These observations can help breeders define robust criteria for identifying genotypes with high resistance and productivity and highlight where genotypes exhibit susceptibility to stress.

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“…On the hormone-free medium, IBL 91/78 clearly surpassed H275 in both rooting rate (Figures 1b and 2b) and the number of roots per explant (Figure 2c). Auxin is the most widely reported hormonal inducer of adventitious root formation both in Populus [43] and in other plant species [44,45]. Furthermore, the positive role of auxin in the promotion of shoot elongation is well reported in Arabidopsis [46], and confirmed in Populus [47].…”

Section: Analysis Of Populus Genotype Responses To the Darkness And G...mentioning

confidence: 90%

Differences in Environmental and Hormonal Regulation of Growth Responses in Two Highly Productive Hybrid Populus Genotypes

Žiauka

Striganavičiūtė

Szyp-Borowska

et al. 2022

Forests

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Phenotypic plasticity, in response to adverse conditions, determines plant productivity and survival. The aim of this study was to test if two highly productive Populus genotypes, characterised by different in vitro etiolation patterns, differ also in their responses to hormones gibberellin (GA) and abscisic acid (ABA), and to a GA biosynthesis inhibitor paclobutrazol (PBZ). The experiments on shoot cultures of ‘Hybrida 275′ (abbr. H275; Populus maximowiczii × P. trichocarpa) and IBL 91/78 (Populus tremula × P. alba) were conducted by either modulating the physical in vitro environment or by adding specific chemicals to the nutrient medium. Our results revealed two main sets of differences between the studied genotypes in environmental and hormonal regulation of growth responses. First, the genotype H275 responded to darkness with PBZ-inhibitable shoot elongation; in contrast, the elongation of IBL 91/78 shoots was not affected either by darkness or PBZ treatment. Secondly, the explants of H275 were unable to recover their growth if it was inhibited with ABA; in contrast, those of IBL 91/78 recovered so well after the temporal inhibition by ABA that, when rooted subsequently, they developed longer shoots and roots than without a previous ABA treatment. Our results indicate that GA catabolism and repressive signalling provide an important pathway to control growth and physiological adaptation in response to immediate or impending adverse conditions. These observations can help breeders define robust criteria for identifying genotypes with high resistance and productivity and highlight where genotypes exhibit susceptibility to stress.

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Effect of indole-3-butyric acid on root formation in Alnus glutinosa microcuttings

Cited by 18 publications

References 50 publications

Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Differences in Environmental and Hormonal Regulation of Growth Responses in Two Highly Productive Hybrid <em>Populus</em> Genotypes

Differences in Environmental and Hormonal Regulation of Growth Responses in Two Highly Productive Hybrid Populus Genotypes

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