Effect ofAntrodiacamphorataon Inflammatory Arterial Thrombosis-Mediated Platelet Activation: The Pivotal Role of Protein Kinase C

Abstract

Antrodia camphorata is a rare Taiwanese medicinal mushroom. Antrodia camphorata extract has been reported to exhibit antioxidant, anti-inflammation, antimetastasis, and anticancer activities and plays a role in liver fibrosis, vasorelaxation, and immunomodulation. Critical vascular inflammation leads to vascular dysfunction and cardiovascular diseases, including abdominal aortic aneurysms, hypertension, and atherosclerosis. Platelet activation plays a crucial role in intravascular thrombosis, which is involved in a wide variety of cardiovascular diseases. However, the effect of Antrodia camphorata on platelet activation remains unclear. We examined the effects of Antrodia camphorata on platelet activation. In the present study, Antrodia camphorata treatment (56–224 μg/mL) inhibited platelet aggregation induced by collagen, but not U46619, an analogue of thromboxane A2, thrombin, and arachidonic acid. Antrodia camphorata inhibited collagen-induced calcium (Ca2+) mobilization and phosphorylation of protein kinase C (PKC) and Akt. In addition, Antrodia camphorata significantly reduced the aggregation and phosphorylation of PKC in phorbol-12, 13-dibutyrate (PDBu) activated platelets. In conclusion, Antrodia camphorata may inhibit platelet activation by inhibiting of Ca2+ and PKC cascade and the Akt pathway. Our study suggests that Antrodia camphorata may be a potential therapeutic agent for preventing or treating thromboembolic disorders.

A preliminary randomised controlled study of short-term Antrodia cinnamomea treatment combined with chemotherapy for patients with advanced cancer

“…Though this observation spanned only a short period, the finding may imply an association between AC and its platelet-lowering effect in patients with lung or gastric cancer. Most research has focused on the antiplatelet activity of AC as a potential therapeutic agent for treating thromboembolic disorders [ 43 , 44 ]. However, no definitive proof demonstrates that AC interferes with the processes of platelet production, destruction, and pooling.…”

Edible mushrooms as potential functional foods in amelioration of hypertension

“…Reduces aggregation and phosphorylation of PKC in phorbol-12,13-dibutyrate-activated platelets (Lu et al, 2014) Cordyceps militaris Cordycepin Alleviates cardiac hypertrophy via AMPKα signaling and reduces oxidative stresses (Wang et al, 2019) S C H E M E 1 Selected bioactive constituents in edible mushrooms in ameliorating hypertension.…”

The Antioxidant Properties of Mushroom Polysaccharides can Potentially Mitigate Oxidative Stress, Beta-Cell Dysfunction and Insulin Resistance

“…Chang was found to significantly decrease platelet aggregation and PKC phosphorylation in platelets activated in phorbol-12, 13-dibutyrate (PDBu) at concentrations of 56—224 μg/ml. Furthermore, polysaccharides inhibited the PKC and Akt pathways during collagen-induced calcium (Ca 2+ ) mobilization ( Lu et al, 2014 ). The authors suggested that oral administration of these mushroom polysaccharides prevented several glucose-induced vascular dysfunctions and inhibited PKC activation, which suggests that mushroom polysaccharides might be an alternative source of antioxidants to treat hyperglycemia-induced oxidative stress.…”