Effect of Hyperthermia on Nonhistone Proteins Isolated with DNA

Tomasovic, Stephen P.; Turner, Gail N.; Dewey, William C.

doi:10.2307/3574956

Cited by 131 publications

(27 citation statements)

References 29 publications

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“…Heat treatment exerts numerous effects on mammalian cells, including chromosome aberrations (Dewey et al, 1971), alterations in chromosomal proteins (Tomasovic et al, 1978), inhibition of DNA repair (Jorritsma and Konings, 1983), damage to membranes (Yatvin, 1977) and the mitotic apparatus (Dewey et al, 1971), and MN induction (Komae et al, 1999). We found no reports, however, regarding the effects of hypothermic conditions on CA or MN induction in mammalian cells, although we previously reported that both elevated and depressed body temperature induced MN in mouse bone marrow cells (Asanami and Shimono, 1997a, 1997b.…”

Section: Introductionmentioning

confidence: 99%

Effect of temperature on the frequency of chromosome aberrations and micronuclei in cultured Chinese hamster cells.

2001

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We previously reported that both hyperthermia and hypothermia induced micronuclei in mouse bone marrow cells (Asanami and Shimono, 1997a, 1997b. To investigate the effects of temperature on chromosome aberration in vitro, we conducted chromosome aberration and micronucleus tests under hyper-and hypothermic conditions using Chinese hamster lung (CHL) cells. In the chromosome aberration test, we observed positive responses at 40 and 41 for 24 hr, and at 42 for 6 hr and over. In the micronucleus test, we observed positive responses at 31 , 33 , and 40 for 24 hr, and at 42 for 2 hr. The results suggest that in CHL cells, hypothermic conditions can induce micronuclei while hyperthermic conditions can induce both chromosome aberrations and micronuclei.

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Section: Introductionmentioning

confidence: 99%

Effect of temperature on the frequency of chromosome aberrations and micronuclei in cultured Chinese hamster cells.

2001

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“…This phenomenon was originally reported for isolated chromatin from CHO (Tomasovic et al, 1978) and HeLa (Roti Roti and Winward, 1978) cells. The excess proteins were found to contain non-histone proteins.…”

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confidence: 96%

Initial characterization of heat‐induced excess nuclear proteins in HeLa cells

Laszlo

Wright

Roti

1992

Journal Cellular Physiology

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Exposure of mammalian cells to hyperthermia is known to cause protein aggregation in the nucleus. The presence of such aggregates has been detected as the relative increase in the protein mass that is associated with nuclei isolated from heated cells. We have characterized these excess nuclear proteins from the nuclei of heated HeLa cells by two-dimensional gel electrophoresis. The abundance of cytoskeletal elements which co-purify with the nuclei did not increase with exposure to hyperthermia, indicating that these proteins are not part of the excess nuclear proteins. In contrast, several specific polypeptides become newly bound or increase in abundance in nuclei isolated from heated cells. Members of the hsp 70 family were identified as a major component of the excess nuclear proteins. Among the other excess nuclear proteins we identified ten that had apparent molecular weights of 130, 95, 75, 58, 53, 48, 46, 37, 28, and 26 kilodaltons. Since hsp 70 is mainly cytoplasmic in non-heated cells, its association with nuclei in heated cells indicates that one mechanism accounting for the heat-induced excess nuclear proteins is the movement of cytoplasmic proteins to the nucleus. We also obtained evidence that increased binding of nuclear proteins is another mechanism for this effect. No overall increase or decrease in the phosphorylation of nuclear proteins was found to be associated with such altered binding or movement from the cytoplasm to the nucleus.

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“…Hyperthermia is known to cause an increase in the nuclear protein mass of heated cells [Tomasovic et al, 1978;Chu et al, 19931. When nuclei are isolated from control and heated cells by using a mild detergent and low salt wash method, there is a greater retention of nuclear protein in the heated cells Warters et al, 19931 suggesting that precipitation or aggregation of nuclear proteins around the DNA and nuclear matrix occurred during heating [Warters et al, 19931.…”

Section: Mechanism For Heat Effects On Dna Replicationmentioning

confidence: 99%

Molecular mechanisms for the induction of chromosomal aberrations in CHO cells heated in S phase

Wong

Dewey

1993

Environ and Mol Mutagen

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Chromosomal aberrations are induced by heat only when Chinese hamster ovary cells are heated in S phase of the cell cycle. Studies on the hyperthermic inhibition of cellular DNA replication have indicated that four molecular aspects of DNA replication are affected after heating. New replicon initiation and DNA chain elongation are inhibited; the fork displacement rate is very sensitive to heat-inactivation; and finally, there is almost a two-fold increase in single-stranded regions in the replicating DNA after heating. From a comparison of these altered processes between S phase cells and heated G1 cells, which do not die from chromosomal aberrations, our current hypothesis involves 3 steps for the chromosomal aberration induction process. The first critical step is the persistent increase of single-stranded regions in the replicating DNA. Then, we hypothesize that the second step is the creation of transient double strand breaks (DSBs) induced at sites opposite these regions by endogenous endonucleases. Finally, the third step requires that improper repair of these DSBs occurs from either nonrepair or misrepair which then leads to the final chromosomal aberrations seen in the first mitosis after treatment. We believe that this 3 step induction process is common for any cytotoxic agent that induces chromosomal aberrations after DNA replication has been inhibited.

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Effect of Hyperthermia on Nonhistone Proteins Isolated with DNA

Cited by 131 publications

References 29 publications

Effect of temperature on the frequency of chromosome aberrations and micronuclei in cultured Chinese hamster cells.

Effect of temperature on the frequency of chromosome aberrations and micronuclei in cultured Chinese hamster cells.

Initial characterization of heat‐induced excess nuclear proteins in HeLa cells

Molecular mechanisms for the induction of chromosomal aberrations in CHO cells heated in S phase

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