Effect of temperature on the frequency of chromosome aberrations and micronuclei in cultured Chinese hamster cells.

Abstract

We previously reported that both hyperthermia and hypothermia induced micronuclei in mouse bone marrow cells (Asanami and Shimono, 1997a, 1997b. To investigate the effects of temperature on chromosome aberration in vitro, we conducted chromosome aberration and micronucleus tests under hyper-and hypothermic conditions using Chinese hamster lung (CHL) cells. In the chromosome aberration test, we observed positive responses at 40 and 41 for 24 hr, and at 42 for 6 hr and over. In the micronucleus test, we observed positive responses at 31 , 33 , and 40 for 24 hr, and at 42 for 2 hr. The results suggest that in CHL cells, hypothermic conditions can induce micronuclei while hyperthermic conditions can induce both chromosome aberrations and micronuclei.

Hyperthermia-induced micronucleus formation in a human keratinocyte cell line

“…The findings of the present study are also in line with several in vitro studies. It was demonstrated in rodent cells, that hyperthermic conditions lead to an increase of chromosome aberrations and micronuclei [15]. Micronucleus frequency was increased fourfold and twenty-fold after exposure to 40 • C and 42 • C respectively (24 h), but no other temperatures were investigated.…”

“…This also includes studies on cytogenetic effects caused by hyperthermia [12][13][14][15]. An interference with the interpretation of toxicological test data has been discovered [16,17], hindering risk assessment of chemicals which influence body temperature.…”

Micronuclei and Nuclear Buds Induced by Cyclophosphamide in Crocodylus moreletii as Useful Biomarkers in Aquatic Environments

“…Regarding the effect on genetic material, we know that exposure to high temperatures increases DNA damage since this macromolecule is denatured at 70 • C. For example, in mice exposed to temperatures above 39.5 • C, an increase in MN was observed in the bone marrow [40]. In this context, the literature describes that temperature exerts numerous effects on cells of living organisms [39], including MN [41]. One of the mechanisms of action proposed in which the increase in temperature affects DNA is that the temperature change disrupts the fibers of the mitotic spindle [40], which is classified as an aneuploidogenic agent.…”

Assessment of DNA damage in Ehlrich carcinoma after treatment with doxorubicin encapsulated in nanoscales thermosensitive liposomes in combination with localized hyperthermia

“…Moreover hyperthermia induces necrosis via mitochondrial swelling followed by rupture of the plasma membrane and release of cytoplasmic constituents, which include protealytic enzymes (Issels 2008). Moreover, Asanami et al (2001), Hintzsche et al (2012), Nadin et al (2012) and Speit and Schütz (2013) had results which indicate that apoptotic cell death is not the predominant biological effect of hyperthermia. Hyperthermia-induced DNA damage has the potential to induce mutations, at least at the chromosomal level.…”