Effect of hydroxyapatite nanoparticles on the growth and p53/c-Myc protein expression of implanted hepatic VX₂ tumor in rabbits by intravenous injection

Abstract

AIM:To evaluate the effect of hydroxyapatite nanoparticles (Nano HAP) by intravenous injection on the inhibition of implanted hepatic VX2 tumor growth in rabbits and cell p53/c-Myc protein expression. METHODS:60 hepatic VX2 tumor-bearing rabbits was randomly divided into five groups. Nano HAP collosol 20 mg/kg, 40 mg/kg, 5-FU solutions 20 mg/mL, mixed liquor of 5-FU solution 20 mg/mL and Nano HAP collosol 20 mg/kg were infused by vein, normal saline conducted as the control. The general state, weight, liver function and gross tumor volume were detected dynamically. The expression of p53 and c-Myc gene protein in tumor tissue was detected by immunohistochemistry methods. RESULTS:The growth of implanted hepatic VX2 tumors was significantly inhibited in all therapy groups, 3 wk after the injection, the tumor control rates in Nano HAP collosol groups were 25.5% and 32.5% respectively, and the gross tumor volumes were obviously less than that of control group. (24.81 ± 5.17 and 22.73 ± 4.23 vs 33.32 ± 5.26, P < 0.05). The tumor control rate of 5-FU group was 43.7% (18.74 ± 4.40 vs 33.32 ± 5.26, P < 0.05), but the general state of the animals after injection aggravated; and the adverse reaction in the drug combination group obviously decreased. Due to the effect of Nano HAP, the positive expression of tumor associated the mutated p53 and c-Myc in tumor tissue was decreased obviously compared with the control group.CONCLUSION: Nano HAP has evident inhibitory action on rabbit implanted hepatic VX2 tumor in vivo , which may be the result of decreasing the expression of the mutated p53 and c-myc, and drug combination can obviously decrease the adverse reaction of 5-FU.

Effect of dilute gelatine on the ultrasonic thermally assisted synthesis of nano hydroxyapatite

“…Whilst Jun et al showed that HAP nanoparticles inhibit the growth of hepatic tumour cells and have stronger anti-tumour effect, Li et al demonstrated a beneficial effect of a nanocomposite of HAP and chitosan on human gastric cancer cells [15][16]. Another advantage of HAP is that it is biocompatible as implants and provides a biological scaffold for bone formation.…”

Sublethal Doses of Inorganic Mercury Induce Dose-Depended Upregulation of RPA1 Content and Inhibit p53 Expression in the Brain of Rainbow Trout (Oncorhynchus mykiss)

“…According literature data, the increase of p53 expression occurs as result cellular response to various types of cell damage. However, several experimental results evidence that the suppression of p53 expression is the mechanism to regulate of transcriptional activity too, especially the inhibition with microRNA or hydroxyapatite nanoparticles (Hu et al, 2007;Cho et al, 2016).…”

Development of a VX2 Pancreatic Cancer Model in Rabbits: A Pilot Study

“…These tumors are well characterized and the method of their induction has been thoroughly studied. Finally, the VX2 molecular biology includes alterations within the function of p53 (31), which also occurs in up to 75% of pancreatic cancers (5). This suggests a degree of molecular homology between the VX2 tumors and human pancreatic tumors, further supporting this model’s utility.…”